The Architecture of Inactivated SARS-CoV-2 with Postfusion Spikes Revealed by Cryo-EM and Cryo-ET.
Chuang Liu,Luiza Mendonça,Yang Yang,Yuanzhu Gao,Chenguang Shen,Jiwei Liu,Tao Ni,Bin Ju,Congcong Liu,Xian Tang,Jinli Wei,Xiaomin Ma,Yanan Zhu,Weilong Liu,Shuman Xu,Yingxia Liu,Jing Yuan,Jing Wu,Zheng Liu,Zheng Zhang,Lei Liu,Peiyi Wang,Peijun Zhang +22 more
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TLDR
This work genetically and structurally characterized β-propiolactone-inactivated viruses from a propagated and purified clinical strain of SARS-CoV-2 and observed that the virus particles are roughly spherical or moderately pleiomorphic, thus resembling a postfusion state.About:
This article is published in Structure.The article was published on 2020-11-03 and is currently open access. It has received 119 citations till now.read more
Citations
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Journal ArticleDOI
Mechanisms of SARS-CoV-2 entry into cells.
TL;DR: In this article, structural and cellular foundations for understanding the multistep SARS-CoV-2 entry process, including S protein synthesis, S protein structure, conformational transitions necessary for association of the spike (S) protein with ACE2, engagement of the receptor-binding domain of the S protein with ACS, proteolytic activation of S protein, endocytosis and membrane fusion are provided.
Journal ArticleDOI
Structural impact on SARS-CoV-2 spike protein by D614G substitution.
Jun Zhang,Jun Zhang,Yongfei Cai,Yongfei Cai,Tianshu Xiao,Tianshu Xiao,Jianming Lu,Hanqin Peng,Sarah M. Sterling,Richard M. Walsh,Sophia Rits-Volloch,Haisun Zhu,Alec N. Woosley,Wei Yang,Piotr Sliz,Piotr Sliz,Bing Chen,Bing Chen +17 more
TL;DR: In this paper, the authors reported the structure of a full-length G614 S trimer, which adopts three distinct prefusion conformations that differ primarily by the position of one receptor-binding domain.
Journal ArticleDOI
Structure-guided multivalent nanobodies block SARS-CoV-2 infection and suppress mutational escape.
Paul-Albert Koenig,Hrishikesh Das,Hejun Liu,Beate M. Kümmerer,Florian N. Gohr,Lea-Marie Jenster,Lisa D. J. Schiffelers,Yonas M. Tesfamariam,Miki Uchima,Jennifer Deborah Wuerth,Karl Gatterdam,Natalia Ruetalo,Maria H Christensen,Caroline I. Fandrey,Sabine Normann,Jan M. P. Tödtmann,Steffen Pritzl,Leo Hanke,Jannik Boos,Meng Yuan,Xueyong Zhu,Jonathan L. Schmid-Burgk,Hiroki Kato,Michael Schindler,Ian A. Wilson,Matthias Geyer,Kerstin U. Ludwig,B. Martin Hallberg,Nicholas C. Wu,Florian I. Schmidt +29 more
TL;DR: In this paper, the authors used x-ray crystallography and cryo-electron microscopy to define two distinct binding epitopes of the SARS-CoV-2 spike protein.
Journal ArticleDOI
Distinguishing features of current COVID-19 vaccines: knowns and unknowns of antigen presentation and modes of action
Franz X. Heinz,Karin Stiasny +1 more
TL;DR: In this paper, the relevance of structural modifications of S in different vaccines and the different modes of antigen expression after vaccination with genetic adenovirus-vector and mRNA vaccines are reviewed.
Journal ArticleDOI
Structural basis for enhanced infectivity and immune evasion of SARS-CoV-2 variants.
Yongfei Cai,Yongfei Cai,Jun Zhang,Jun Zhang,Tianshu Xiao,Tianshu Xiao,Christy L. Lavine,Shaun Rawson,Hanqin Peng,Haisun Zhu,Krishna Anand,Pei Tong,Avneesh Gautam,Shen Lu,Sarah M. Sterling,Richard M. Walsh,Sophia Rits-Volloch,Jianming Lu,Duane R. Wesemann,Wei Yang,Michael S. Seaman,Bing Chen,Bing Chen +22 more
TL;DR: In this article, the full-length spike (S) trimers of the B.1.7 and B.351 variants of SARS-CoV-2 have been analyzed.
References
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Journal ArticleDOI
UCSF Chimera--a visualization system for exploratory research and analysis.
Eric F. Pettersen,Thomas D. Goddard,Conrad C. Huang,Gregory S. Couch,Daniel M. Greenblatt,Elaine C. Meng,Thomas E. Ferrin +6 more
TL;DR: Two unusual extensions are presented: Multiscale, which adds the ability to visualize large‐scale molecular assemblies such as viral coats, and Collaboratory, which allows researchers to share a Chimera session interactively despite being at separate locales.
Journal ArticleDOI
A Novel Coronavirus from Patients with Pneumonia in China, 2019.
Na Zhu,Dingyu Zhang,Wenling Wang,Xingwang Li,Bo Yang,Jingdong Song,Xiang Zhao,Baoying Huang,Weifeng Shi,Roujian Lu,Peihua Niu,Faxian Zhan,Xuejun Ma,Dayan Wang,Wenbo Xu,Wenbo Xu,Guizhen Wu,George F. Gao,Wenjie Tan +18 more
TL;DR: Human airway epithelial cells were used to isolate a novel coronavirus, named 2019-nCoV, which formed a clade within the subgenus sarbecovirus, Orthocoronavirinae subfamily, which is the seventh member of the family of coronaviruses that infect humans.
Journal ArticleDOI
SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor
Markus Hoffmann,Hannah Kleine-Weber,Simon Schroeder,Nadine Krüger,Tanja Herrler,Sandra Erichsen,Tobias S. Schiergens,Georg Herrler,Nai Huei Wu,Andreas Nitsche,Marcel A. Müller,Christian Drosten,Christian Drosten,Stefan Pöhlmann +13 more
TL;DR: It is demonstrated that SARS-CoV-2 uses the SARS -CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming, and it is shown that the sera from convalescent SARS patients cross-neutralized Sars-2-S-driven entry.
Journal ArticleDOI
Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation.
Daniel Wrapp,Nianshuang Wang,Kizzmekia S. Corbett,Jory A. Goldsmith,Ching-Lin Hsieh,Olubukola M. Abiona,Barney S. Graham,Jason S. McLellan +7 more
TL;DR: The authors show that this protein binds at least 10 times more tightly than the corresponding spike protein of severe acute respiratory syndrome (SARS)–CoV to their common host cell receptor, and test several published SARS-CoV RBD-specific monoclonal antibodies found that they do not have appreciable binding to 2019-nCoV S, suggesting that antibody cross-reactivity may be limited between the two RBDs.
Journal ArticleDOI
The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2
Alexander E. Gorbalenya,Susan C. Baker,Ralph S. Baric,Raoul J. de Groot,Christian Drosten,Anastasia A. Gulyaeva,Bart L. Haagmans,Chris Lauber,Andrey M. Leontovich,Benjamin W. Neuman,Dmitry Penzar,Stanley Perlman,Leo L.M. Poon,Dmitry V. Samborskiy,Igor A. Sidorov,Isabel Sola,John Ziebuhr +16 more
TL;DR: The independent zoonotic transmission of SARS-CoV and SARS -CoV-2 highlights the need for studying viruses at the species level to complement research focused on individual pathogenic viruses of immediate significance.
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