The eIF3 interactome reveals the translasome, a supercomplex linking protein synthesis and degradation machineries.
Zhe Sha,Laurence M. Brill,Rodrigo Cabrera,Oded Kleifeld,Judith S. Scheliga,Michael H. Glickman,Eric C. Chang,Dieter A. Wolf +7 more
TLDR
The data reveal the breadth of the eIF3 interactome and suggest that factors involved in translation initiation, ribosome biogenesis, translation elongation, quality control, and transport are physically linked to facilitate efficient protein synthesis.About:
This article is published in Molecular Cell.The article was published on 2009-10-09 and is currently open access. It has received 138 citations till now. The article focuses on the topics: Initiation factor & Interactome.read more
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Journal ArticleDOI
Proteasomal and Autophagic Degradation Systems.
TL;DR: This review summarizes molecular details of how proteasome and autophagy pathways are functionally interconnected in cells and indicates common principles and nodes of communication that can be therapeutically exploited.
Journal ArticleDOI
Nuclear translation visualized by ribosome-bound nascent chain puromycylation
Alexandre David,Brian P. Dolan,Heather D. Hickman,Jonathan J. Knowlton,Giovanna Clavarino,Giovanna Clavarino,Giovanna Clavarino,Philippe Pierre,Philippe Pierre,Philippe Pierre,Jack R. Bennink,Jonathan W. Yewdell +11 more
TL;DR: A new method for visualizing translation in cells via standard immunofluorescence microscopy provides evidence for translation in the nucleoplasm and nucleolus.
Journal ArticleDOI
Assembly and Function of Heterotypic Ubiquitin Chains in Cell-Cycle and Protein Quality Control.
Richard G. Yau,Richard G. Yau,Kerstin Doerner,Erick R. Castellanos,Diane L. Haakonsen,Diane L. Haakonsen,Achim Werner,Nan Wang,Nan Wang,X. William Yang,X. William Yang,Nadia Martinez-Martin,Marissa L. Matsumoto,Vishva M. Dixit,Michael Rape,Michael Rape +15 more
TL;DR: It is shown that K11/K48-linked chains are synthesized and processed by essential ubiquitin ligases and effectors that are mutated across neurodegenerative diseases; accordingly, these conjugates promote rapid proteasomal clearance of aggregation-prone proteins.
Journal ArticleDOI
Cdc48/p97 promotes degradation of aberrant nascent polypeptides bound to the ribosome
TL;DR: It is concluded that budding yeast Cdc48 functions downstream of the Ub ligases Ltn1 and Ubr1 to release nascent proteins from the ribosome so that they can be degraded by the proteasome.
Journal ArticleDOI
Principles of cotranslational ubiquitination and quality control at the ribosome.
TL;DR: It is found that quality control at the ribosome is achieved through a tiered system wherein nascent polypeptides have a chance to fold before becoming accessible to ubiquitination.
References
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Journal ArticleDOI
Global landscape of protein complexes in the yeast Saccharomyces cerevisiae
Nevan J. Krogan,Gerard Cagney,Gerard Cagney,Haiyuan Yu,Gouqing Zhong,Xinghua Guo,Alexandr Ignatchenko,Joyce Li,Shuye Pu,Nira Datta,Aaron Tikuisis,Thanuja Punna,José M. Peregrín-Alvarez,Michael Shales,Xin Zhang,Michael Davey,Mark D. Robinson,Alberto Paccanaro,James E. Bray,Anthony Sheung,Bryan Beattie,Dawn Richards,Veronica Canadien,Atanas Iliev Lalev,Frank Mena,Peter D Wong,Andrei Starostine,Myra M. Canete,James Vlasblom,Samuel Wu,Chris Orsi,Sean R. Collins,Shamanta Chandran,Robin Haw,Jennifer J. Rilstone,Kiran Gandi,Natalie J. Thompson,Gabe Musso,Peter St Onge,Shaun Ghanny,Mandy H. Y. Lam,Gareth Butland,Amin M. Altaf-Ul,Shigehiko Kanaya,Ali Shilatifard,Erin K. O'Shea,Jonathan S. Weissman,C. James Ingles,Timothy P. Hughes,John Parkinson,Mark Gerstein,Shoshana J. Wodak,Andrew Emili,Jack Greenblatt +53 more
TL;DR: T tandem affinity purification was used to process 4,562 different tagged proteins of the yeast Saccharomyces cerevisiae to identify protein–protein interactions, which will help future studies on individual proteins as well as functional genomics and systems biology.
Journal ArticleDOI
Proteome survey reveals modularity of the yeast cell machinery
Anne-Claude Gavin,Patrick Aloy,Paola Grandi,Roland Krause,Markus Boesche,Martina Marzioch,Christina Rau,Lars Juhl Jensen,Sonja Bastuck,Birgit Dümpelfeld,Angela Edelmann,Marie-Anne Heurtier,Verena Hoffman,Christian Hoefert,Karin Klein,Manuela Hudak,Anne-Marie Michon,Malgorzata Schelder,Markus Schirle,Marita Remor,Tatjana Rudi,Sean D. Hooper,Andreas Bauer,Tewis Bouwmeester,Georg Casari,Gerard Drewes,Gitte Neubauer,Jens Rick,Bernhard Kuster,Peer Bork,Robert B. Russell,Giulio Superti-Furga +31 more
TL;DR: This study reports the first genome-wide screen for complexes in an organism, budding yeast, using affinity purification and mass spectrometry and provides the largest collection of physically determined eukaryotic cellular machines so far and a platform for biological data integration and modelling.
Journal ArticleDOI
A Model for Random Sampling and Estimation of Relative Protein Abundance in Shotgun Proteomics
TL;DR: A linear dynamic range over 2 orders of magnitude is demonstrated by using the number of spectra (spectral sampling) acquired for each protein by the data-dependent acquisition of peptides eluting into the mass spectrometer.
Journal ArticleDOI
Transport between the cell nucleus and the cytoplasm.
Dirk Görlich,Ulrike Kutay +1 more
TL;DR: A focus of this review is nuclear export of messenger RNA, which apparently largely relies on export mediators distinct from importin beta-related factors.
Journal ArticleDOI
Rapid degradation of a large fraction of newly synthesized proteins by proteasomes
Ulrich S. Schubert,Luis C. Antón,James P. Gibbs,Christopher C. Norbury,Jonathan W. Yewdell,Jack R. Bennink +5 more
TL;DR: It is shown that ubiquitinated DRiPs are formed from human immunodeficiency virus Gag polyprotein, a long-lived viral protein that serves as a source of antigenic peptides.