The roles of the human SETMAR (Metnase) protein in illegitimate DNA recombination and non-homologous end joining repair.
Michael Tellier,Ronald Chalmers +1 more
TLDR
Full length SETMAR expression has no effect on DNA repair and integration in vivo and SETMAR putative nuclease activity is not required in vivo.About:
This article is published in DNA Repair.The article was published on 2019-08-01 and is currently open access. It has received 16 citations till now. The article focuses on the topics: DNA repair & Non-homologous end joining.read more
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The Role of Histone Lysine Methylation in the Response of Mammalian Cells to Ionizing Radiation.
TL;DR: In this paper, the role of histone lysine methylation changes in regulating the response to radiation-induced genotoxic damage in mammalian cells is reviewed and the effects of the modulation of their expression and/or the pharmacological inhibition of their activity on the radio-sensitivity of different cell lines.
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Distinct roles of structure-specific endonucleases EEPD1 and Metnase in replication stress responses
Neelam Sharma,Michael Clayton Speed,Christopher P. Allen,David G. Maranon,Elizabeth A. Williamson,Sudha Singh,Robert Hromas,Jac A. Nickoloff +7 more
TL;DR: It is proposed that EEPD1 initiates fork restart by cleaving stalled forks, and that Metnase may promote forks restart by processing homologous recombination intermediates and/or inducing H3K36me2 to recruit DDR factors.
Journal ArticleDOI
Compensating for over-production inhibition of the Hsmar1 transposon in Escherichia coli using a series of constitutive promoters
TL;DR: A set of vectors was constructed based on constitutive promoters of different strengths to provide a wide range of transposase expression which will be useful for screening libraries ofTransposase mutants and found that mutations in the Hsmar1 dimer interface provide resistance to OPI in bacteria, which could be valuable for improving bacterial transposon mutagenesis techniques.
Posted ContentDOI
Development of a papillation assay using constitutive promoters to find hyperactive transposases
Michael Tellier,Ronald Chalmers +1 more
TL;DR: An improvement of the well-known papillation assay is presented where in place of an inducible promoter, a set of constitutive promoters cloned into a one or five copies vector in presence or absence of a ribosome binding site is designed.
Journal ArticleDOI
Two repeated motifs enriched within some enhancers and origins of replication are bound by SETMAR isoforms in human colon cells
Aymeric Antoine-Lorquin,Peter Arensburger,Ahmed Arnaoty,Sassan Asgari,Martine Batailler,Linda Beauclair,Catherine Belleannée,Nicolas Buisine,Vincent Coustham,Serge Guyétant,Laura Helou,Thierry Lecomte,Bruno Pitard,Isabelle Stévant,Yves Bigot +14 more
TL;DR: The role of SETMAR in the expression of genes, DNA replication and in DNA repair is discussed in this article, where the authors profiled SETMAR expression and its genome-wide binding landscape in colon tissue.
References
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Tissue-based map of the human proteome
Mathias Uhlén,Mathias Uhlén,Linn Fagerberg,Björn M. Hallström,Cecilia Lindskog,Per Oksvold,Adil Mardinoglu,Åsa Sivertsson,Caroline Kampf,Evelina Sjöstedt,Evelina Sjöstedt,Anna Asplund,IngMarie Olsson,Karolina Edlund,Emma Lundberg,Sanjay Navani,Cristina Al-Khalili Szigyarto,Jacob Odeberg,Dijana Djureinovic,Jenny Ottosson Takanen,Sophia Hober,Tove Alm,Per-Henrik Edqvist,Holger Berling,Hanna Tegel,Jan Mulder,Johan Rockberg,Peter Nilsson,Jochen M. Schwenk,Marica Hamsten,Kalle von Feilitzen,Mattias Forsberg,Lukas Persson,Fredric Johansson,Martin Zwahlen,Gunnar von Heijne,Jens Nielsen,Jens Nielsen,Fredrik Pontén +38 more
TL;DR: In this paper, a map of the human tissue proteome based on an integrated omics approach that involves quantitative transcriptomics at the tissue and organ level, combined with tissue microarray-based immunohistochemistry, to achieve spatial localization of proteins down to the single-cell level.
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Histone methyltransferase activity associated with a human multiprotein complex containing the Enhancer of Zeste protein
TL;DR: The isolation of a multiprotein E(z) complex that contains extra sex combs, suppressor of zeste-12, and the histone binding proteins RbAp46/RbAp48 is reported, which possesses HMT activity with specificity for Lys 9 (K9) and Lys 27 (K27) of histone H3.
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Non-homologous DNA end joining and alternative pathways to double-strand break repair.
TL;DR: This Review discusses the most recent findings regarding the relative involvement of the different NHEJ proteins in the repair of various DNA-end configurations and the relevance of these different pathways to human disease.
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The quantitative proteome of a human cell line
Martin Beck,Alexander Schmidt,Johan Malmstroem,Manfred Claassen,Alessandro Ori,Anna Szymborska,Franz Herzog,Oliver Rinner,Jan Ellenberg,Ruedi Aebersold,Ruedi Aebersold +10 more
TL;DR: This work provides a quantitative description of the proteome of a commonly used human cell line in two functional states, interphase and mitosis, and shows that these human cultured cells express at least ∼10 000 proteins and that the quantified proteins span a concentration range of seven orders of magnitude up to 20 000 000 copies per cell.
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Regulation of MLL1 H3K4 methyltransferase activity by its core components
Yali Dou,Thomas A. Milne,Alexander J. Ruthenburg,Alexander J. Ruthenburg,Seunghee Lee,Jae Woon Lee,Gregory L. Verdine,C. David Allis,Robert G. Roeder +8 more
TL;DR: This study reports the first biochemical reconstitution of a functional four-component mixed-lineage leukemia protein-1 (MLL1) core complex and demonstrates that WDR5 mediates interactions of the MLL1 catalytic unit both with the common structural platform and with the histone substrate.