Journal ArticleDOI
Visualizing dendritic cell networks in vivo.
Randall L Lindquist,Guy Shakhar,Diana Dudziak,Hedda Wardemann,Thomas Eisenreich,Thomas Eisenreich,Michael L. Dustin,Michel C. Nussenzweig,Michel C. Nussenzweig +8 more
TLDR
Two-photon microscopy of lymph nodes in live mice showed that most of the steady-state DCs were enmeshed in an extensive network and remained in place while actively probing adjacent T cells with their processes.Abstract:
In the steady state, dendritic cells (DCs) in the lymph node induce T cell tolerance to self antigens. Innate signals trigger the maturation of tissue DCs, which migrate into lymph nodes and activate T cells. To examine DCs in vivo, we produced transgenic mice whose DCs expressed enhanced yellow fluorescent protein. Two-photon microscopy of lymph nodes in live mice showed that most of the steady-state DCs were enmeshed in an extensive network and remained in place while actively probing adjacent T cells with their processes. Mature DCs were more motile than steady-state DCs and were rapidly dispersed and integrated into the sessile network, facilitating their interaction with migrating T cells.read more
Citations
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Journal ArticleDOI
Taking dendritic cells into medicine
TL;DR: Some medical implications of DC biology that account for illness and provide opportunities for prevention and therapy are presented.
Journal ArticleDOI
From Monocytes to M1/M2 Macrophages: Phenotypical vs. Functional Differentiation
Paola Italiani,Diana Boraschi +1 more
TL;DR: This review will address some of the important questions under the general framework of the role of monocytes and macrophages in the initiation, development, resolution, and chronicization of inflammation.
Journal ArticleDOI
Rapid leukocyte migration by integrin-independent flowing and squeezing
Tim Lämmermann,Bernhard L. Bader,Susan J. Monkley,Tim Worbs,Roland Wedlich-Söldner,Karin Hirsch,Markus Keller,Reinhold Förster,David R. Critchley,Reinhard Fässler,Michael Sixt +10 more
TL;DR: It is shown here that functional integrins do not contribute to migration in three-dimensional environments, and these cells migrate by the sole force of actin-network expansion, which promotes protrusive flowing of the leading edge.
Journal ArticleDOI
Monocyte subsets differentially employ CCR2, CCR5, and CX3CR1 to accumulate within atherosclerotic plaques
Frank Tacke,David Alvarez,Theodore J. Kaplan,Claudia Jakubzick,Rainer Spanbroek,Jaime Llodra,Alexandre Garin,Jianhua Liu,Matthias Mack,Nico van Rooijen,Sergio A. Lira,Andreas J. R. Habenicht,Gwendalyn J. Randolph +12 more
TL;DR: Analyzing mouse monocyte subsets in apoE-deficient mice and tracing their differentiation and chemokine receptor usage as they accumulated within atherosclerotic plaques suggests antagonizing CX3CR1 may be effective therapeutically in ameliorating CCR2(+) monocyte recruitment to plaques without impairing their C CR2-dependent responses to inflammation overall.
Journal ArticleDOI
Dendritic-cell trafficking to lymph nodes through lymphatic vessels
TL;DR: Gaining a better understanding of the crosstalk between dendritic cells and lymphatic vessels during the migration of d endritic cells to lymph nodes is essential for future advances in manipulating dendrite-cell migration as a means to fine-tune immune responses in clinical settings.
References
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Journal ArticleDOI
Tolerogenic dendritic cells.
TL;DR: It is suggested that several clinical situations, including autoimmunity and certain infectious diseases, can be influenced by the antigen-specific tolerogenic role of DCs.
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A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications
TL;DR: The development of an improved version of YFP named Venus, which contains a novel mutation, F46L, which at 37°C greatly accelerates oxidation of the chromophore, the rate-limiting step of maturation and will enable fluorescent labelings that were not possible before.
Journal ArticleDOI
Green mice' as a source of ubiquitous green cells
TL;DR: Transgenic mouse lines with an ‘enhanced’ GFP (EGFP) cDNA under the control of a chicken beta‐actin promoter and cytomegalovirus enhancer were produced and all of the tissues from these transgenic lines, with the exception of erythrocytes and hair, were green under excitation light.
Journal ArticleDOI
Mouse and human dendritic cell subtypes
Ken Shortman,Yong-Jun Liu +1 more
TL;DR: The dynamics of the DC network in response to microbial invasion is studied, because many DC subtypes arise from separate developmental pathways, and their development and function are modulated by exogenous factors.