Journal ArticleDOI
Wiring specificity in the direction-selectivity circuit of the retina
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It is shown, using serial block-face electron microscopy and two-photon calcium imaging, that the dendrites of mouse starburst amacrine cells make highly specific synapses with direction-selective ganglion cells depending on the ganglION cell’s preferred direction.Abstract:
The proper connectivity between neurons is essential for the implementation of the algorithms used in neural computations, such as the detection of directed motion by the retina. The analysis of neuronal connectivity is possible with electron microscopy, but technological limitations have impeded the acquisition of high-resolution data on a large enough scale. Here we show, using serial block-face electron microscopy and two-photon calcium imaging, that the dendrites of mouse starburst amacrine cells make highly specific synapses with direction-selective ganglion cells depending on the ganglion cell's preferred direction. Our findings indicate that a structural (wiring) asymmetry contributes to the computation of direction selectivity. The nature of this asymmetry supports some models of direction selectivity and rules out others. It also puts constraints on the developmental mechanisms behind the formation of synaptic connections. Our study demonstrates how otherwise intractable neurobiological questions can be addressed by combining functional imaging with the analysis of neuronal connectivity using large-scale electron microscopy.read more
Citations
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Journal ArticleDOI
Direction selectivity of the retinotectal system of fish: Findings based on microelectrode extracellular recordings of the tectum opticum
Ilija Damjanović,Alexey T. Aliper,Paul Maximov,Alisa A. Zaichikova,Zoran Gačić,Elena Maximova +5 more
TL;DR: In this article , the authors compared the results of other authors examining direction selectivity in the fish retinotectal system and found that the preferred directions of three different types of direction-selective tectal neurons (DS TNs) match the preferred direction of three types of DS GCs.
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Connecting Connectomes to Physiology
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Machine Learning and Optimization for Neural Circuit Reconstruction
TL;DR: An iterative coarse-to-fine procedure that optimizes the parameters of spatially vary linear transformations of the intensity data in order to minimize discontinuities along the section axis, subject to detail-preserving regularization is proposed.
Posted ContentDOI
Visual experience instructs dendrite orientation but is not required for asymmetric wiring of the retinal direction selective circuit
TL;DR: Dendritic form follows proper circuit function, where dendritic orientation is refined over the course of development and is dependent on structured visual experience following eye opening, as observed in vDSGCs of normally reared animals.
Posted ContentDOI
Special Nuclear Layer Contacts Among Starburst Amacrine Cells in the Mouse Retina
Shang Mu,Nicholas L. Turner,William Silversmith,Chris S. Jordan,Nico Kemnitz,Marissa Sorek,Celia David,Devon Jones,Doug Bland,Merlin Moore,Amy L. R. Sterling,H. Sebastian Seung +11 more
TL;DR: Novel classes of cell-cell contacts between retinal starburst amacrine neurons are observed, from finely detailed morphological reconstructions of cells from an electron microscopic image volume of a mouse retina.
References
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Journal ArticleDOI
Two-Photon Laser Scanning Fluorescence Microscopy
TL;DR: The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
Journal ArticleDOI
User-guided 3D active contour segmentation of anatomical structures: Significantly improved efficiency and reliability
Paul A. Yushkevich,Joseph Piven,Heather C. Hazlett,Rachel Gimpel Smith,Sean Ho,James C. Gee,Guido Gerig +6 more
TL;DR: The methods and software engineering philosophy behind this new tool, ITK-SNAP, are described and the results of validation experiments performed in the context of an ongoing child autism neuroimaging study are provided, finding that SNAP is a highly reliable and efficient alternative to manual tracing.
Journal ArticleDOI
The structure of the nervous system of the nematode Caenorhabditis elegans
TL;DR: The structure and connectivity of the nervous system of the nematode Caenorhabditis elegans has been deduced from reconstructions of electron micrographs of serial sections as discussed by the authors.
Journal ArticleDOI
The mechanism of directionally selective units in rabbit's retina.
Horace Barlow,W. R. Levick +1 more
TL;DR: Experiments are described which show, first, that directional selectivity is not due to optical aberrations of some kind and, secondly, that it is not a simple matter of the latency of response varying systematically across the receptive field.
Journal ArticleDOI
Serial block−face scanning electron microscopy to reconstruct three−dimensional tissue nanostructure
Winfried Denk,Heinz Horstmann +1 more
TL;DR: It is demonstrated that datasets meeting these requirements can be obtained by automated block-face imaging combined with serial sectioning inside the chamber of a scanning electron microscope, opening the possibility of automatically obtaining the electron-microscope-level 3D datasets needed to completely reconstruct the connectivity of neuronal circuits.
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