Journal ArticleDOI
Wiring specificity in the direction-selectivity circuit of the retina
TLDR
It is shown, using serial block-face electron microscopy and two-photon calcium imaging, that the dendrites of mouse starburst amacrine cells make highly specific synapses with direction-selective ganglion cells depending on the ganglION cell’s preferred direction.Abstract:
The proper connectivity between neurons is essential for the implementation of the algorithms used in neural computations, such as the detection of directed motion by the retina. The analysis of neuronal connectivity is possible with electron microscopy, but technological limitations have impeded the acquisition of high-resolution data on a large enough scale. Here we show, using serial block-face electron microscopy and two-photon calcium imaging, that the dendrites of mouse starburst amacrine cells make highly specific synapses with direction-selective ganglion cells depending on the ganglion cell's preferred direction. Our findings indicate that a structural (wiring) asymmetry contributes to the computation of direction selectivity. The nature of this asymmetry supports some models of direction selectivity and rules out others. It also puts constraints on the developmental mechanisms behind the formation of synaptic connections. Our study demonstrates how otherwise intractable neurobiological questions can be addressed by combining functional imaging with the analysis of neuronal connectivity using large-scale electron microscopy.read more
Citations
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Consistency in predicting functions from anatomical and functional connectivity profiles across the cortical cortex
TL;DR: The current results provided the first comprehensive picture of the relationships between functions and connectivity in the whole human cortex at a fine-grained brain atlas.
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Sample Preparation and Warping Accuracy for Correlative Multimodal Imaging in the Mouse Olfactory Bulb Using 2-Photon, Synchrotron X-Ray and Volume Electron Microscopy
Yu-Xing Zhang,Tobias Ackels,Alexandra Pacureanu,Marie-Christine Zdora,Anne Bonnin,Andreas T. Schaefer,Carles Bosch +6 more
TL;DR: It is demonstrated that direct, single-cell precise correlation between in vivo 2P and SXRT tissue volumes is possible and as reliable as correlating between 2p and SBEM and pave the way for experiments that require retrieving physiology, circuit structure and synaptic signatures in targeted regions.
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Improving the Usability of Virtual Reality Neuron Tracing with Topological Elements.
Torin McDonald,Will Usher,Nate Morrical,Attila Gyulassy,Steve Petruzza,Frederick Federer,Alessandra Angelucci,Valerio Pascucci +7 more
TL;DR: A new semi-automatic method that uses topological features to guide users in tracing neurons and integrate this method within a virtual reality (VR) framework previously used for manual tracing is proposed.
Journal ArticleDOI
Dendrite Morphology Minimally Influences the Synaptic Distribution of Excitation and Inhibition in Retinal Direction-Selective Ganglion Cells.
TL;DR: In this paper, the authors compare two morphologically distinct retinal direction-selective ganglion cells (DSGCs): directionally tuned inhibition and spatially offset inhibition.
Journal ArticleDOI
An Artificial Visual System for Three Dimensional Motion Direction Detection
Mianzhe Han,Yuki Todo,Zheng Tang +2 more
TL;DR: Wang et al. as mentioned in this paper proposed a new 3D artificial visual system to extend motion direction detection mechanism into 3D space, where a neuron can detect the local motion of a single voxel object within 3D spaces.
References
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Journal ArticleDOI
Two-Photon Laser Scanning Fluorescence Microscopy
TL;DR: The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
Journal ArticleDOI
User-guided 3D active contour segmentation of anatomical structures: Significantly improved efficiency and reliability
Paul A. Yushkevich,Joseph Piven,Heather C. Hazlett,Rachel Gimpel Smith,Sean Ho,James C. Gee,Guido Gerig +6 more
TL;DR: The methods and software engineering philosophy behind this new tool, ITK-SNAP, are described and the results of validation experiments performed in the context of an ongoing child autism neuroimaging study are provided, finding that SNAP is a highly reliable and efficient alternative to manual tracing.
Journal ArticleDOI
The structure of the nervous system of the nematode Caenorhabditis elegans
TL;DR: The structure and connectivity of the nervous system of the nematode Caenorhabditis elegans has been deduced from reconstructions of electron micrographs of serial sections as discussed by the authors.
Journal ArticleDOI
The mechanism of directionally selective units in rabbit's retina.
Horace Barlow,W. R. Levick +1 more
TL;DR: Experiments are described which show, first, that directional selectivity is not due to optical aberrations of some kind and, secondly, that it is not a simple matter of the latency of response varying systematically across the receptive field.
Journal ArticleDOI
Serial block−face scanning electron microscopy to reconstruct three−dimensional tissue nanostructure
Winfried Denk,Heinz Horstmann +1 more
TL;DR: It is demonstrated that datasets meeting these requirements can be obtained by automated block-face imaging combined with serial sectioning inside the chamber of a scanning electron microscope, opening the possibility of automatically obtaining the electron-microscope-level 3D datasets needed to completely reconstruct the connectivity of neuronal circuits.
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