Journal ArticleDOI
Wiring specificity in the direction-selectivity circuit of the retina
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TLDR
It is shown, using serial block-face electron microscopy and two-photon calcium imaging, that the dendrites of mouse starburst amacrine cells make highly specific synapses with direction-selective ganglion cells depending on the ganglION cell’s preferred direction.Abstract:
The proper connectivity between neurons is essential for the implementation of the algorithms used in neural computations, such as the detection of directed motion by the retina. The analysis of neuronal connectivity is possible with electron microscopy, but technological limitations have impeded the acquisition of high-resolution data on a large enough scale. Here we show, using serial block-face electron microscopy and two-photon calcium imaging, that the dendrites of mouse starburst amacrine cells make highly specific synapses with direction-selective ganglion cells depending on the ganglion cell's preferred direction. Our findings indicate that a structural (wiring) asymmetry contributes to the computation of direction selectivity. The nature of this asymmetry supports some models of direction selectivity and rules out others. It also puts constraints on the developmental mechanisms behind the formation of synaptic connections. Our study demonstrates how otherwise intractable neurobiological questions can be addressed by combining functional imaging with the analysis of neuronal connectivity using large-scale electron microscopy.read more
Citations
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Analogous Convergence of Sustained and Transient Inputs in Parallel On and Off Pathways for Retinal Motion Computation
TL;DR: Evidence is found that sustained and transient On BC types are wired to On SAC dendrites at different distances from the SAC soma, mirroring the previous wiring diagram for the Off BC-SAC circuit, consistent with the hypothesis that On and Off pathways contain parallel correlation-type motion detectors.
Journal ArticleDOI
Motion-Detecting Circuits in Flies: Coming into View
TL;DR: An outline of the neural pathways that compute visual motion that are functional similarities between motion-processing pathways in different animals, despite profound differences in circuit anatomy and structure is described.
Journal ArticleDOI
A comparison of neuronal population dynamics measured with calcium imaging and electrophysiology.
Ziqiang Wei,Ziqiang Wei,Bei Jung Lin,Bei Jung Lin,Tsai Wen Chen,Tsai Wen Chen,Kayvon Daie,Karel Svoboda,Shaul Druckmann,Shaul Druckmann +9 more
TL;DR: This work compared neuronal spikes and fluorescence in matched neural populations in behaving mice and developed a model transforming spike trains to synthetic-imaging data, which highlights challenges in relating electrophysiology and imaging data and suggests forward modeling as an effective way to understand differences between these data.
Journal ArticleDOI
Correlative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical neurons.
Bohumil Maco,Anthony Holtmaat,Marco Cantoni,Anna Kreshuk,Christoph N. Straehle,Fred A. Hamprecht,Graham Knott +6 more
TL;DR: This work describes a semi-automated technique whereby volumes of brain tissue containing axons and dendrites are subsequently imaged in three dimensions with focused ion beam scanning electron microcopy and neurites are identified and reconstructed automatically from the image series using the latest segmentation algorithms.
Journal ArticleDOI
Extracellular space preservation aids the connectomic analysis of neural circuits
Marta Pallotto,Paul V. Watkins,Boma Fubara,Joshua H. Singer,Kevin L. Briggman,Kevin L. Briggman +5 more
TL;DR: This work explored preserving extracellular space (ECS) during chemical tissue fixation to improve the ability to segment neurites and to identify synaptic contacts and determined that antibodies penetrate deep into ECS preserved tissue with only minimal permeabilization, thereby enabling correlated light microscopy (LM) and EM studies.
References
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Journal ArticleDOI
Two-Photon Laser Scanning Fluorescence Microscopy
TL;DR: The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
Journal ArticleDOI
User-guided 3D active contour segmentation of anatomical structures: Significantly improved efficiency and reliability
Paul A. Yushkevich,Joseph Piven,Heather C. Hazlett,Rachel Gimpel Smith,Sean Ho,James C. Gee,Guido Gerig +6 more
TL;DR: The methods and software engineering philosophy behind this new tool, ITK-SNAP, are described and the results of validation experiments performed in the context of an ongoing child autism neuroimaging study are provided, finding that SNAP is a highly reliable and efficient alternative to manual tracing.
Journal ArticleDOI
The structure of the nervous system of the nematode Caenorhabditis elegans
TL;DR: The structure and connectivity of the nervous system of the nematode Caenorhabditis elegans has been deduced from reconstructions of electron micrographs of serial sections as discussed by the authors.
Journal ArticleDOI
The mechanism of directionally selective units in rabbit's retina.
Horace Barlow,W. R. Levick +1 more
TL;DR: Experiments are described which show, first, that directional selectivity is not due to optical aberrations of some kind and, secondly, that it is not a simple matter of the latency of response varying systematically across the receptive field.
Journal ArticleDOI
Serial block−face scanning electron microscopy to reconstruct three−dimensional tissue nanostructure
Winfried Denk,Heinz Horstmann +1 more
TL;DR: It is demonstrated that datasets meeting these requirements can be obtained by automated block-face imaging combined with serial sectioning inside the chamber of a scanning electron microscope, opening the possibility of automatically obtaining the electron-microscope-level 3D datasets needed to completely reconstruct the connectivity of neuronal circuits.
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