Journal ArticleDOI
Wiring specificity in the direction-selectivity circuit of the retina
TLDR
It is shown, using serial block-face electron microscopy and two-photon calcium imaging, that the dendrites of mouse starburst amacrine cells make highly specific synapses with direction-selective ganglion cells depending on the ganglION cell’s preferred direction.Abstract:
The proper connectivity between neurons is essential for the implementation of the algorithms used in neural computations, such as the detection of directed motion by the retina. The analysis of neuronal connectivity is possible with electron microscopy, but technological limitations have impeded the acquisition of high-resolution data on a large enough scale. Here we show, using serial block-face electron microscopy and two-photon calcium imaging, that the dendrites of mouse starburst amacrine cells make highly specific synapses with direction-selective ganglion cells depending on the ganglion cell's preferred direction. Our findings indicate that a structural (wiring) asymmetry contributes to the computation of direction selectivity. The nature of this asymmetry supports some models of direction selectivity and rules out others. It also puts constraints on the developmental mechanisms behind the formation of synaptic connections. Our study demonstrates how otherwise intractable neurobiological questions can be addressed by combining functional imaging with the analysis of neuronal connectivity using large-scale electron microscopy.read more
Citations
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Posted ContentDOI
Dimensionality in recurrent spiking networks: global trends in activity and local origins in connectivity
Stefano Recanatesi,Gabriel Koch Ocker,Michael A. Buice,Michael A. Buice,Eric Shea-Brown,Eric Shea-Brown +5 more
TL;DR: Here, a set of principles are derived that inform how the connectivity of a spiking neural network determines the dimensionality of the activity that it produces, and how stimuli and intrinsic connectivity interact in shaping the overall dimension of a network’s response.
Posted ContentDOI
Feedforward inhibition allows input summation to vary in recurrent cortical networks
TL;DR: Optogenetic manipulations in awake mice expose how a transformation in a cortical network depends on internal network activity, and that transformation depend critically on feedforward inhibition – the fact that inputs to the cortex often make strong connections on both excitatory and inhibitory neurons.
Journal ArticleDOI
Visual Experience Influences Dendritic Orientation but Is Not Required for Asymmetric Wiring of the Retinal Direction Selective Circuit.
TL;DR: Interestingly, vDSGCs in dark-reared mice lose their inhibition-independent dendritic contribution to direction-selective tuning while maintaining asymmetric inhibitory input, which indicates that different mechanisms of a cell's computational abilities can be constructed over development through divergent mechanisms.
Journal ArticleDOI
Glutamate Activity Regulates and Dendritic Development of J-RGCs.
TL;DR: It is shown that the dendrites of J-RGCs are strictly ramified in the outer portion of the inner plexiform layer (IPL) of the retina at the age of postnatal day 8 (P8), mimicking the ramification pattern of adults.
Journal ArticleDOI
Combining secondary ion mass spectrometry image depth profiling and single particle inductively coupled plasma mass spectrometry to investigate the uptake and biodistribution of gold nanoparticles in Caenorhabditis elegans
Monique E. Johnson,Joe Bennett,Antonio R. Montoro Bustos,Shannon K. Hanna,Andrei Kolmakov,Nicholas E. Sharp,Elijah J. Petersen,Patricia E. Lapasset,Christopher M. Sims,Karen E. Murphy,Bryant C. Nelson +10 more
TL;DR: The complementary data from both SIMS image depth profiling and spICP-MS provides a complete view of the uptake, translocation, and size distribution of ingested NPs within Caenorhabditis elegans.
References
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Journal ArticleDOI
Two-Photon Laser Scanning Fluorescence Microscopy
TL;DR: The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
Journal ArticleDOI
User-guided 3D active contour segmentation of anatomical structures: Significantly improved efficiency and reliability
Paul A. Yushkevich,Joseph Piven,Heather C. Hazlett,Rachel Gimpel Smith,Sean Ho,James C. Gee,Guido Gerig +6 more
TL;DR: The methods and software engineering philosophy behind this new tool, ITK-SNAP, are described and the results of validation experiments performed in the context of an ongoing child autism neuroimaging study are provided, finding that SNAP is a highly reliable and efficient alternative to manual tracing.
Journal ArticleDOI
The structure of the nervous system of the nematode Caenorhabditis elegans
TL;DR: The structure and connectivity of the nervous system of the nematode Caenorhabditis elegans has been deduced from reconstructions of electron micrographs of serial sections as discussed by the authors.
Journal ArticleDOI
The mechanism of directionally selective units in rabbit's retina.
Horace Barlow,W. R. Levick +1 more
TL;DR: Experiments are described which show, first, that directional selectivity is not due to optical aberrations of some kind and, secondly, that it is not a simple matter of the latency of response varying systematically across the receptive field.
Journal ArticleDOI
Serial block−face scanning electron microscopy to reconstruct three−dimensional tissue nanostructure
Winfried Denk,Heinz Horstmann +1 more
TL;DR: It is demonstrated that datasets meeting these requirements can be obtained by automated block-face imaging combined with serial sectioning inside the chamber of a scanning electron microscope, opening the possibility of automatically obtaining the electron-microscope-level 3D datasets needed to completely reconstruct the connectivity of neuronal circuits.
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