Journal ArticleDOI
Wiring specificity in the direction-selectivity circuit of the retina
TLDR
It is shown, using serial block-face electron microscopy and two-photon calcium imaging, that the dendrites of mouse starburst amacrine cells make highly specific synapses with direction-selective ganglion cells depending on the ganglION cell’s preferred direction.Abstract:
The proper connectivity between neurons is essential for the implementation of the algorithms used in neural computations, such as the detection of directed motion by the retina. The analysis of neuronal connectivity is possible with electron microscopy, but technological limitations have impeded the acquisition of high-resolution data on a large enough scale. Here we show, using serial block-face electron microscopy and two-photon calcium imaging, that the dendrites of mouse starburst amacrine cells make highly specific synapses with direction-selective ganglion cells depending on the ganglion cell's preferred direction. Our findings indicate that a structural (wiring) asymmetry contributes to the computation of direction selectivity. The nature of this asymmetry supports some models of direction selectivity and rules out others. It also puts constraints on the developmental mechanisms behind the formation of synaptic connections. Our study demonstrates how otherwise intractable neurobiological questions can be addressed by combining functional imaging with the analysis of neuronal connectivity using large-scale electron microscopy.read more
Citations
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Journal ArticleDOI
Dendro-dendritic cholinergic excitation controls dendritic spike initiation in retinal ganglion cells
TL;DR: Dendro-dendritic release of neurotransmitters from SACs bi-directionally gate dendritic spike initiation to control the directionally selective action potential output of retinal ganglion cells.
Journal ArticleDOI
Automated image computing reshapes computational neuroscience
TL;DR: Several critical issues in current computational neuroscience are identified, and opinions on potential solutions based on bioimage informatics, especially automated image computing are presented.
Journal ArticleDOI
Cross-synaptic synchrony and transmission of signal and noise across the mouse retina
TL;DR: This approach reveals that synchronization of rod bipolar cell synapses is near perfect in the dark and decreases with increasing light level, revealing how cross-synaptic synchrony shapes retinal responses to physiological light inputs and, more generally, signaling in complex neural networks.
Journal ArticleDOI
A segregated cortical stream for retinal direction selectivity.
TL;DR: The authors show that specific connectivity of cortical neurons routes retina-originated direction-selective signaling into distinct HVAs, which identifies a cortical processing stream for motion computed in the retina.
References
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Journal ArticleDOI
Two-Photon Laser Scanning Fluorescence Microscopy
TL;DR: The fluorescence emission increased quadratically with the excitation intensity so that fluorescence and photo-bleaching were confined to the vicinity of the focal plane as expected for cooperative two-photon excitation.
Journal ArticleDOI
User-guided 3D active contour segmentation of anatomical structures: Significantly improved efficiency and reliability
Paul A. Yushkevich,Joseph Piven,Heather C. Hazlett,Rachel Gimpel Smith,Sean Ho,James C. Gee,Guido Gerig +6 more
TL;DR: The methods and software engineering philosophy behind this new tool, ITK-SNAP, are described and the results of validation experiments performed in the context of an ongoing child autism neuroimaging study are provided, finding that SNAP is a highly reliable and efficient alternative to manual tracing.
Journal ArticleDOI
The structure of the nervous system of the nematode Caenorhabditis elegans
TL;DR: The structure and connectivity of the nervous system of the nematode Caenorhabditis elegans has been deduced from reconstructions of electron micrographs of serial sections as discussed by the authors.
Journal ArticleDOI
The mechanism of directionally selective units in rabbit's retina.
Horace Barlow,W. R. Levick +1 more
TL;DR: Experiments are described which show, first, that directional selectivity is not due to optical aberrations of some kind and, secondly, that it is not a simple matter of the latency of response varying systematically across the receptive field.
Journal ArticleDOI
Serial block−face scanning electron microscopy to reconstruct three−dimensional tissue nanostructure
Winfried Denk,Heinz Horstmann +1 more
TL;DR: It is demonstrated that datasets meeting these requirements can be obtained by automated block-face imaging combined with serial sectioning inside the chamber of a scanning electron microscope, opening the possibility of automatically obtaining the electron-microscope-level 3D datasets needed to completely reconstruct the connectivity of neuronal circuits.
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