Yeast-expressed recombinant protein of the receptor-binding domain in SARS-CoV spike protein with deglycosylated forms as a SARS vaccine candidate
Wen-Hsiang Chen,Lanying Du,Shivali M. Chag,Cuiqing Ma,Nancy Tricoche,Xinrong Tao,Christopher A. Seid,Elissa M. Hudspeth,Sara Lustigman,Chien Te K. Tseng,Maria Elena Bottazzi,Peter J. Hotez,Bin Zhan,Shibo Jiang +13 more
Reads0
Chats0
TLDR
It is found that RBD219-N1 exhibited high expression yield, and maintained its antigenicity and functionality, and more importantly, induced significantly stronger RBD-specific antibody responses and a higher level of neutralizing antibodies in immunized mice than RBD193-WT, RBD 193-N 1, R BD193-N3, or RBD 219-WT.Abstract:
Development of vaccines for preventing a future pandemic of severe acute respiratory syndrome (SARS) caused by SARS coronavirus (SARS-CoV) and for biodefense preparedness is urgently needed. Our pr...read more
Citations
More filters
Journal ArticleDOI
Deep Mutational Scanning of SARS-CoV-2 Receptor Binding Domain Reveals Constraints on Folding and ACE2 Binding.
Tyler N. Starr,Allison J. Greaney,Allison J. Greaney,Sarah K Hilton,Sarah K Hilton,Daniel Ellis,Katharine H.D. Crawford,Katharine H.D. Crawford,Adam S. Dingens,Mary Jane Navarro,John E. Bowen,M. Alejandra Tortorici,Alexandra C. Walls,Neil P. King,David Veesler,Jesse D. Bloom,Jesse D. Bloom,Jesse D. Bloom +17 more
TL;DR: It is found that a substantial number of mutations to the RBD are well tolerated or even enhance ACE2 binding, including at ACE2 interface residues that vary across SARS-related coronaviruses.
Journal ArticleDOI
Characterization of the receptor-binding domain (RBD) of 2019 novel coronavirus: implication for development of RBD protein as a viral attachment inhibitor and vaccine.
Wanbo Tai,Lei He,Xiujuan Zhang,Jing Pu,Jing Pu,Denis Voronin,Shibo Jiang,Shibo Jiang,Yusen Zhou,Lanying Du +9 more
TL;DR: The receptor-binding domain (RBD) in SARS-CoV-2 S protein was identified and it was found that the RBD protein bound strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors and could block the binding and, hence, attachment of SARs-Cov-2 RBD and SARS -CoV RBD to ACE2-expressing cells, thus inhibiting their infection to host cells.
Journal ArticleDOI
Unique epidemiological and clinical features of the emerging 2019 novel coronavirus pneumonia (COVID-19) implicate special control measures.
TL;DR: The latest literatures on genetic, epidemiological, and clinical features of COVID‐19 in comparison to SARS and MERS are summarized and special measures on diagnosis and potential interventions are emphasized to improve understanding of the unique features ofCOVID‐ 19 and enhance control measures in the future.
Journal ArticleDOI
Complete Mapping of Mutations to the SARS-CoV-2 Spike Receptor-Binding Domain that Escape Antibody Recognition.
Allison J. Greaney,Allison J. Greaney,Tyler N. Starr,Pavlo Gilchuk,Seth J. Zost,Elad Binshtein,Andrea N. Loes,Andrea N. Loes,Sarah K Hilton,John Huddleston,Rachel Eguia,Katharine H.D. Crawford,Katharine H.D. Crawford,Adam S. Dingens,Rachel S. Nargi,Rachel E. Sutton,Naveenchandra Suryadevara,Paul W. Rothlauf,Paul W. Rothlauf,Zhuoming Liu,Sean P. J. Whelan,Robert H. Carnahan,Robert H. Carnahan,James E. Crowe,James E. Crowe,Jesse D. Bloom,Jesse D. Bloom,Jesse D. Bloom +27 more
TL;DR: A deep mutational scanning method is described to map how all amino-acid mutations in the RBD affect antibody binding, and this method is applied to 10 human monoclonal antibodies to enable rational design of antibody therapeutics and assessment of the antigenic consequences of viral evolution.
Journal ArticleDOI
Coronavirus Infections in Children Including COVID-19: An Overview of the Epidemiology, Clinical Features, Diagnosis, Treatment and Prevention Options in Children.
Petra Zimmermann,Nigel Curtis +1 more
TL;DR: Preliminary evidence suggests children are just as likely as adults to become infected with SARS-CoV-2 but are less likely to be symptomatic or develop severe symptoms, but the importance of children in transmitting the virus remains uncertain.
References
More filters
Journal ArticleDOI
Coronavirus as a possible cause of severe acute respiratory syndrome
Jsm Peiris,Sik To Lai,Llm Poon,Yi Guan,Lyc Yam,Wilina Lim,John M. Nicholls,Wks Yee,WW Yan,MT Cheung,V. C. C. Cheng,KH Chan,Dnc Tsang,R Yung,Ting Kin Ng,KY Yuen +15 more
TL;DR: Serological and molecular tests specific for the virus permitted a definitive laboratory diagnosis to be made and allowed further investigation to define whether other cofactors play a part in disease progression.
Journal ArticleDOI
Structure of SARS Coronavirus Spike Receptor-Binding Domain Complexed with Receptor
Fang Li,Wenhui Li,Wenhui Li,Michael Farzan,Michael Farzan,Stephen C. Harrison,Stephen C. Harrison +6 more
TL;DR: The structure of the RBD suggests ways to make truncated disulfide-stabilized RBD variants for use in the design of coronavirus vaccines.
Journal ArticleDOI
The spike protein of SARS-CoV — a target for vaccine and therapeutic development
TL;DR: Recent advances in the development of vaccines and therapeutics based on the S protein are highlighted, which plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV.
Journal ArticleDOI
Epidemiology and cause of severe acute respiratory syndrome (SARS) in Guangdong, People's Republic of China, in February, 2003
Nanshan Zhong,Bo-Jian Zheng,Yimin Li,Leo L.M. Poon,Z. H. Xie,KH Chan,Philip H. Li,S. Y. Tan,Qingsong Chang,J. P. Xie,X. Q. Liu,J. Xu,D. X. Li,Kwok-Yung Yuen,Joseph S. M. Peiris,Yi Guan +15 more
TL;DR: Genetic analysis showed that the SARS CoV isolates from Guangzhou shared the same origin with those in other countries, and had a phylogenetic pathway that matched the spread of SARS to the other parts of the world.
Journal ArticleDOI
A 193-Amino Acid Fragment of the SARS Coronavirus S Protein Efficiently Binds Angiotensin-converting Enzyme 2
TL;DR: It is demonstrated that a 193-amino acid fragment of the S protein bound ACE2 more efficiently than did the full S1 domain (residues 12–672) and a point mutation at aspartic acid 454 abolished association of the fullS1 domain and of the 193-residue fragment with ACE2.
Related Papers (5)
A pneumonia outbreak associated with a new coronavirus of probable bat origin
Peng Zhou,Xing-Lou Yang,Xian Guang Wang,Ben Hu,Lei Zhang,Wei Zhang,Hao Rui Si,Yan Zhu,Bei Li,Chao Lin Huang,Hui-Dong Chen,Jing Chen,Yun Luo,Hua Guo,Ren Di Jiang,Meiqin Liu,Ying Chen,Xu Rui Shen,Xi Wang,Xiao Shuang Zheng,Kai Zhao,Quanjiao Chen,Fei Deng,Lin Lin Liu,Bing Yan,Fa Xian Zhan,Yan-Yi Wang,Gengfu Xiao,Zhengli Shi +28 more