Applied Biosystems

About: Applied Biosystems is a based out in . It is known for research contribution in the topics: Mass spectrometry & Nucleic acid. The organization has 1521 authors who have published 1579 publications receiving 285423 citations.

Nanocapillary liquid chromatography interfaced to tandem matrix-assisted laser desorption/ionization and electrospray ionization-mass spectrometry: Mapping the nuclear proteome of human fibroblasts.

Abstract: Miniaturized liquid chromatography nanoseparation in combination with minigel fractionation of human primary cell nuclei is presented. We obtained high-sensitivity and high-throughput identification of expressed proteins by subcellular fractionation and nanocapillary liquid chromatography interfaced to both electrospray ionization (ESI)- and matrix-assisted laser desorption/ionisation (MALDI) tandem mass spectrometry. The reversed-phase nanocapillary eluents were applied directly onto the MALDI target plate as discrete crystal spots using in-line matrix infusion. When working with primary cells, only a limited amount of sample is available. To maximize the number of identified proteins from a restricted amount of sample, miniaturized sample preparation protocols and nanoflow separation is a necessity, especially when working with low-abundant proteins. From the same isolated nuclear sample, complementary separation of intact proteins by two-dimensional (2-D) gel electrophoresis was made. In total 594 gene products from the nuclear preparations were identified out of which 261 were unique. Several proteins involved in transcriptional events were identified such as TATA-binding protein, EBNA-co-activator, and interleukin enhancer binding proteins, indicating that sufficient proteomic depth is obtained to study transcriptional controlling events. Our results suggest that by sample prefractionation and downscaled nanoflow separation along with a combined mass spectrometry strategy, it is possible to identify a large number of nuclear proteins from human primary cells. These findings are of particular importance due to the disease link of these targets cells.

PNA probes, probe sets, methods and kits pertaining to the detection of microorganisms

Abstract: This invention is related to novel PNA probes, probe sets, methods and kits pertaining to the detection of microorganisms. The probes, probe sets, methods and kits of this invention can be used to detect, identify or quantitate one or more organisms in a sample wherein the organisms are selected from the group consisting of E. coli, Staphylococcus aureus, Pseudomonas aeruginosa, Pseudomonas cepatia, Pseudomonas fluorescens or organisms of a bacterial genus including the Salmonella genus, Bacillus genus or Pseudomonas genus. The preferred probing nucleobase sequence of the PNA probes used to detect the bacteria listed above are TCA-ATG-AGC-AAA-GGT ( E. coli ); GCT-TCT-CGT-CCG-TTC ( Staphylococcus aureus ); CTG-AAT-CCA-GGA-GCA and AAC-TTG-CTG-AAC-CAC ( Pseudomonas aeruginosa ); CCA-TCG-CAT-CTA-ACA ( Pseudomonas cepatia ); TCT-AGT-CAG-TCA-GTT ( Pseudomonas fluorescens ); CCG-ACT-TGA-CAG-ACC and CCT-GCC-AGT-TTC-GAA (Salmonella genus); CTT-TGT-TCT-GTC-CAT (Bacillus genus); GCT-GGC-CTA-GCC-TTC, GTC-CTC-CTT-GCG-GTT and TTC-TCA-TCC-GCT-CGA (Pseudomonas genus). The PNA probes, probe sets, methods and kits of this invention are particularly well suited for use in multiplex PNA-FISH assays.

PTH Amino Acid Analysis

Abstract: The chemical process, employed by automated protein/peptide sequencers is derived from the technique originated by Pehr Edman in the 1950s for the sequential degradation of peptide chains.1, 2. The first step in this degradation is selective coupling of a peptide’s amino-terminal amino acid with the Edman reagent, phenylisothiocyanate (PITC), a reaction catalyzed by an organic base delivered with the coupling reagent. The second step is cleavage of this derivatized amino acid from the remainder of the peptide, a reaction effected by treating the peptide with a strong organic acid. Each repeated coupling/cleavage cycle occurs at the newly-formed amino-terminal amino acid left by the previous cycle. Thus, repetitive cycles provide sequential separation of the amino acids which form the primary structure of the peptide.