Applied Biosystems

About: Applied Biosystems is a based out in . It is known for research contribution in the topics: Mass spectrometry & Nucleic acid. The organization has 1521 authors who have published 1579 publications receiving 285423 citations.

Crystal and solution structures of the oligonucleotide d(ATGCGCAT)2: a combined X-ray and NMR study

Abstract: A combined crystal-structure determination and NMR analysis of the octanucleotide d(ATGCGCAT)2 is reported The X-ray analysis shows that the structure is A-form duplex in crystal state The NMR study shows that in solution this sequence is B-type The conformational results from each technique are presented in detail The implications of these findings in terms of conformational flexibility and ligand binding are discussed

Extended rhodamine compounds useful as fluorescent labels

Abstract: Extended rhodamine compounds exhibiting favorable fluorescence characteristics having structure (I) or (II) are disclosed. In addition, novel intermediates for synthesis of these dyes are disclosed, such intermediates having structure (III). In addition, methods of making and using the dyes as fluorescent labels are disclosed.

Phenyl xanthene dyes

Abstract: Fluorescent phenyl xanthene dyes are described that comprise any fluorescein, rhodamine or rhodol comprising a particular C9 phenyl ring. One or both of the ortho groups on the lower C9 phenyl ring is ortho substituted with a group selected from alkyl, heteroalkyl, alkoxy, halo, haloalkyl, amino, mercapto, alkylthio, cyano, isocyano, cyanato, mercaptocyanato, nitroso, nitro, azido, sulfeno, sulfinyl, and sulfino. In one embodiment, halo and/or hydroxy groups are used. Optimal dyes contain a lower C9 phenyl ring in which both ortho groups are the same and the lower ring exhibits some form a symmetry relative to an imaginary axis running from the phenyl rings point of attachment to the remainder of the xanthene dye through a point para to the point of attachment. The phenyl xanthene dyes may be activated. Furthermore, the phenyl xanthene dyes may be conjugated to one or more substances including other dyes. The phenyl xanthene dyes are useful for a number of purposes, including labels for use in automated DNA sequencing as well the formation of fluorescent “bar codes” for polymeric particles used in the multiplexed analysis of analytes.

Antifouling coatings for optoelectronic tweezers.

Abstract: Optoelectronic tweezers enables parallel manipulation of individual single cells using optical addressing and optically induced dielectrophoretic force. This provides a useful platform for performing a variety of biological functions, such as cell manipulation, cell sorting, and cell electroporation. However, in order to obtain more reliable cellular manipulation, especially of adherent mammalian cells, antifouling coatings need to be used to avoid non-specific cell adherence. Two antifouling coatings are discussed here, which can reduce the amount of non-specific adherence by as much as a factor of 30.