Applied Biosystems

About: Applied Biosystems is a based out in . It is known for research contribution in the topics: Mass spectrometry & Nucleic acid. The organization has 1521 authors who have published 1579 publications receiving 285423 citations.

The Sequence of Acheta Adipokinetic Hormone and the Variation in Corpus cardiacum Content and Hyperlipaemic Response with Age

Abstract: The principle neuropeptide separated by reversed-phase liquid chromatography RP -HPLC from extracts of the corpora cardica of Acheta domesticus showed strong adipokinetic activity when injected into Acheta. The N -terminal pyroglutamate of the peptide was removed by enzymatic digestion, and the remaining peptide sequenced. The structure is identical to the peptide Grb -AKH previously described from the corpus cardiacum (CC) of Gryllus bimaculatus (pGlu - Val - Asn - Phe - Ser - Thr - Gly - Trp - NH₂). The ED₅₀ was (0.8 pmol) and saturation was achieved with injection of 2 pmol of synthetic Grb-AKH . The time to maximum hyperlipaemic response was 90 -120 min. The response of the fat body to injected synthetic Grb- AKH doubled in 4 days during the last stadium , but was never greater than half the maximum response of the adult stage. The adult adipokinetic response doubled from the first to the fourth day then gradually declined through day 16. The increased AKH response was timecorrelated to fat storage in the larvae and to lipid diposits in the oocytes in the adult. Synthetic Grb -AKH activated glycogen phosphorylase in the fat body of Acheta. The amount of Grb- AKH present in the CC changed very little throughout the last larval stadium and through the first 9 days of the adult stage, averaging about 15 pmol/gland pair. A second peptide (a hexadecapeptide) was isolated from the CC of Acheta and sequenced. Its structure is identical to a putative diuretic hormone previously described in Acheta.

Closing blade for deformable valve in a microfluidic device and method

Abstract: A microfluidic manipulation system is provided that includes a blade for manipulating deformable material and at least one movable support that is capable of moving the blade into contact with a microfluidic device including a deformable feature. When the microfluidic device is operatively held by a holder, a movable support can position the distal end of the blade relative to the microfluidic device and move the contact tip surface of the blade such that it deforms the deformable feature.

Dispenser array spotting

Abstract: A method of dispensing a fluid comprising (a) aspirating a first fluid volume into a dispenser adapted to dispense fluid volumes of one microliter or less; (b) dispensing a desired volume of the fluid volume to form a dispensed portion; (c) calculating the volume of the dispensed portion; and (d) calculating an adjusted desired volume that compensates for a difference between the desired volume and the volume of the dispensed portion.

Ectopic expression of reelin alters migration of sympathetic preganglionic neurons in the spinal cord.

Abstract: Reelin, an extracellular matrix molecule, regulates neuronal positioning in the brain, brainstem, and spinal cord. Although Reelin was identified more than a decade ago, its function on neuronal migration is still poorly understood. Using a transgenic mouse that expressed reelin under the nestin promoter, we examined here the function of Reelin in control of sympathetic preganglionic neurons (SPN) migration in the spinal cord. SPN undergo primary and secondary migration to arrive at their final locations. In wildtype mice, postmitotic SPN undergo primary migration from the neuroepithelium to the ventrolateral spinal cord, and then undergo a secondary dorsal migration to their final location to form the intermediolateral column (IML). In reeler, which lacks Reelin, SPN also undergo primary migration to the ventrolateral spinal cord as in wildtype. However, during secondary migration, SPN migrate medially to cluster adjacent to the central canal. Our present study on transgenic rl/rl mutants (rl/rl ne-reelin) shows that the initial migration of SPN (embryonic day [E]9.5-E12.5) was similar to reeler. SPN migrated from the neuroepithelium to the ventrolateral spinal cord and then back toward the central canal, despite strong reelin expression in the ventricular zone. However, SPN did not aggregate near the central canal when ectopic reelin was expressed. Only when the expression level of ectopic reelin in the ventricular zone became very weak (E18.5) were SPN found to cluster near the central canal. Postnatally, SPN in rl/rl ne-reelin transgenic mice were located in both the IML and near the central canal. These results show that SPN position can change with location and level of reelin expression. Possible functions of Reelin on SPN migration are discussed.