Dr. Hari Singh Gour University

About: Dr. Hari Singh Gour University is a education organization based out in Saugor, Madhya Pradesh, India. It is known for research contribution in the topics: Drug delivery & Computer science. The organization has 1120 authors who have published 1315 publications receiving 29511 citations. The organization is also known as: Dr Harisingh Gour Vishwavidyalaya & Sagar University.

CeO2 nanoparticle-modified electrode as a novel electrochemical interface in the quantification of Zn2+ ions at trace level: application to real sample analysis

Abstract: A simple strategy has been proposed to quantify Zn2+ ions using CeO2 nanoparticle-modified glassy carbon electrode. The CeO2 nanoparticles were prepared by sucrose-nitrate decomposition method, and it was characterized by X-ray diffraction (XRD), FT-IR, TEM, and surface area analyzer. The synthesized CeO2 nanoparticles were used as modifier molecules as a thin film on glassy carbon electrode (GCE) in the trace level quantification of Zn2+ by using cyclic voltammetry (CV) and differential pulse anodic stripping voltammetry (DPASV) techniques. The fabricated sensor exhibited a good analytical response towards Zn2+ ions. The modified electrode showed a wide linearity in the concentration range 20–380 μg L−1 with a limit of detection 0.36 μg L−1. The proposed electrochemical sensor was successfully applied to trace level Zn2+ quantification from real sample matrices.

A simple, rapid and sensitive spectrofluorimetric method for the determination of camptothecin

Abstract: Camptothecin (CPT) represents a clinically useful class of anticancer agent. Proper identification and quantitation of the CPT in the plant extracts and in-vitro cell culture extracts is fundamental to assess the CPT content and its biosynthetic potential in plants. A simple, sensitive and rapid, spectrofluorimetric method has been developed and validated for the quantitative estimation of camptothecin. The method was validated in terms of linearity (2–20 ng/ml), precision (intra-day variation below 0.15, inter-day variation below 1.2), and accuracy (98.0 to 100.2%). The limit of detection and limit of quantification for CPT were found to be 0.10 ng/ml and 0.36 ng/ml, respectively. The developed spectrofluorimetric method provides a rapid and cost effective method for the routine analysis of CPT in plant extracts and tissue culture samples. The developed method was successfully used for the estimation of CPT in natural plant extracts and cell culture extracts. The Nothapodytes nimmoniana callus cells having nearly 3-fold higher CPT content over the leaf (0.005%) explant of the plant. The highest CPT content was found in the stem part (0.092%) followed by the fruit (0.088%). The method is simple, sensitive and precise; it can be used for the routine quality control testing of formulations containing CPT.

Salbutamol Delivering Transdermal Dosage Form Based on Osmo-Regulatory Principle

Abstract: A transdermal drug delivery system of Salbutamol was developed which released the drug following zero order kinetics. The designed system essentially based on trilaminated matrix concept. The delivery of drug from the system affected by osmotic phenomenon where sodium chloride was used as an osmogent. To establish the desired release rate polyethylene glycol 4000 (PEG 4000) was used as channelising agent in rate controlling membrance of cellulose acetate. The designed systems exhibiting zero-order release rate, were studied for the in-vitro skin permeation. The product which was having skin permeability rate 115 mcg/hr/cm2 was selected for the in-vivo evaluation. The forced expiratory volume (FEV1) and drug plasma concentration were monitored periodically. The study revealed that designed osmoregulatory transdermal drug delivery system of Salbutamol could be used successfully with improved therapeutic response and holds promise for the clinical studies.

Pharmacological evaluation of calendula officinalis L. on bronchial asthma in various experimental animals

Abstract: Background: Calendula officinalis Linn. (Asteraceae) is an aromatic herb growing in the forests of India, China, Central Europe, and some tropical areas. The study was designed to investigate the scientific basis for the traditional claim of C. Officinalis on asthma. In the present study, methanol extract of whole plants was evaluated for preliminary phytochemical screening and antiasthmatic activity on different animals. Subjects and Methods: The asthmatic activity was evaluated by histamine- or acetylcholine-induced bronchospasm in guinea pigs, compound 48/80-induced mast cell degranulation in wistar rats, histamine-induced constriction on isolated guinea pig trachea, and ovalbumin-induced sensitization in mice at different dose levels of C. Officinalis. The preconvulsion dyspnoea time at 0 th and 7 th days at the dose of 250 and 500 mg/kg in guinea pig, the percentage of granulated and degranulated mast cell of at the dose of 600, 800, 1000 μg/mL in rats, muscular contraction at the dose of 600, 800, and 1000 μg/mL on isolated guinea pig trachea and the inflammatory cell count, that is, eosinophils, neutrophills, lymphocytes, macrophages, interleukin (IL)-4, IL-5, and immunoglobulin-E (IgE) from bronchoalveolar lavage fluid at the dose levels of 50, 100, and 250 mg/kg in mice were evaluated and compared with respective control groups. Results: Phytochemical studies revealed the presence of flavonoids, steroids, saponin, terpenoids, lignins, and phenolic compounds in the extract. In addition, the treatment of methanolic extract of C. officinalis (MECO) significantly (P < 0.001) decreased the bronchospasm induced by histamine or acetylcholine in guinea pigs, degranulation mast cell in rats, histamine-induced constriction on isolated guinea pig trachea, and the level of inflammatory cells as compared with inducer groups. The antiasthmatic activity was potentiated in all the doses in dose-dependent manner. Conclusion: The present study concludes that the antiasthmatic activity may be due to the presence of above phytoconstituents by antihistaminic, anticholinergic, antispasmodic, and mast cell stabilizing property.

Journey of Hydrocotyle sibthorpioides Lam.: From traditional utilization to modern therapeutics-A review.

Abstract: Hydrocotyle sibthorpioides Lam. (Araliaceae) is a short and edible medicinal herb, which is used in the traditional system of medicines. The review aims to report the current information of H. sibthorpioides on the basis of its botanical and taxonomical description, traditional use, active phytoconstituents, pharmacological use and toxicity. The information on H. sibthorpioides with respect to its journey from traditional uses to scientific validation was gathered based on the online survey. The results from the review signify that the plant is used by the different tribes of the world for the treatment of patients suffering from fever, edema, dysentery, rheumatalgia, whooping cough, jaundice, throat pain, psoriasis, herpes zoster infection, hepatitis-B infection, soothing pain, dysmenorrhoea and carbunculosis. It is also used as a brain tonic, detoxifying agent and hepatoprotective agent. Scientific investigation reported that the plant has a significant pharmacological activity, viz. cognitive agent, anti-cancer, antiviral, antibacterial, anti-fungal and hepatoprotective. Moreover, based on the phytochemical aspects, a total of 50 phytoconstituents was identified and isolated from the plant. In conclusion, the outcome of this review will be useful for (a) developing a comprehensive plant profile; (b) assist investigators for exploring further research; and (c) to fulfil the gaps lacking in terms of clinical studies.