Dr. Hari Singh Gour University

About: Dr. Hari Singh Gour University is a education organization based out in Saugor, Madhya Pradesh, India. It is known for research contribution in the topics: Drug delivery & Computer science. The organization has 1120 authors who have published 1315 publications receiving 29511 citations. The organization is also known as: Dr Harisingh Gour Vishwavidyalaya & Sagar University.

Inhibitory potency of withania somnifera extracts against dpp-4: an in vitro evaluation

Abstract: Background: Pharmacologic treatments for type 2 diabetes are based upon increasing insulin availability and improving sensitivity to insulin. Nowadays, glucagon like peptide-1 (GLP-1) based therapies aims at glucose control through DPP-4 inhibitors. DPP-4 is a transmembrane glycoprotein belongs to prolyl oligopeptidase family, with the specificity of removing X-Pro or X-Ala dipeptides from the N-terminus of polypeptides. GLP-1 effect by stimulating glucose-dependent insulin release from the pancreatic islets, inhibit inappropriate post-meal glucagon release and slow gastric emptying promoting leaky gut. The current study investigated DPP-4 inhibitory activity of catechin, isolated from Withania somnifera (WS), for ethnopharmacological treatment of type 2 diabetes and aimed to increase availability of GLP-1and sensitivity to insulin. Materials and Methods: Young and matured fresh roots, leaves, and fruits of WS plant extract were considered and were systematically evaluated for DPP-4 inhibitory activity using in vitro method, enzyme kinetics, phytochemical analysis, RP-HPLC, LCMS and 1H and 13C NMR method and structure-activity relationship (SAR) studies. Results: In this study, methanol (100% and 80%) extracts of WS matured root exhibited maximum DPP-4 inhibitory activity when compared to other extracts. The maximum DPP-4 inhibitory activity was found in 100% methanol extract of matured root. Phytobioactive was purified by RP-HPLC. The compound purified was found to be flavonoid and was characterized (LCMS, 1H and 13C NMR studies), identified as catechin. Auxiliary, molecular docking was performed using Ligand Fit method using PatchDock package. The study revealed the binding affinity of catechin with DPP-4 to be -6.601 kcal/mol with 13 hydrogen interactions with the receptor and was very similar to the standard potent blockers withaferin A and others (cuscohygrine, scopoletin, sitoindoside IV, tropine), further confirming its hyperglycemic potency. Conclusion: The study reveals that, 100% methanol extract of WS matured roots contains the compound- catechin, which exhibits DPP-4 inhibitory activity resulting in increased level of bioactive GLP-1 and GIP. In this background, we concluded that the WS will be a better source for further development as new antidiabetic drugs.

Prediction of anti-HIV activity and cytotoxicity of pyrimidinyl and triazinyl amines: A QSAR study

Abstract: A QSAR study on a series of pyrimidinyl and triazinyl amines was performed to explore the physico-chemical parameters responsible for their anti-HIV activity and cytotoxicity. Physico-chemical parameters were calculated using WIN CAChe 6.1. Stepwise multiple linear regression analysis was carried out to derive QSAR models which were further evaluated for statistical significance and predictive power by internal and external validation. The selected best QSAR models showed correlation coefficient R of 0.914 and 0.901, and cross-validated squared correlation coefficient Q2 of 0.685 and 0.691 for anti-HIV activity and cytotoxicity, respectively. The developed significant QSAR model indicates that hydrophobicity of the whole molecule plays an important role in the anti-HIV activity and cytotoxicity of pyrimidinyl and triazinyl amine derivatives. When hydrophobicity is increased, anti-HIV activity of the present series of compounds is decreased leading to high cytotoxicity.

Characterization of Banana and Bagasse Fiber-Reinforced Hybrid Epoxy Composites

Abstract: In this work, banana and bagasse fibers have been taken as reinforcement material because of its ease of availability and low cost. Initially, the density of the fibers are found using water displacement method. The fibers were treated by NaOH and NaCl solution of 5% concentration for good adhesion property. These are reinforced with the epoxy with Hardener—HY 951. The 20 and 30% of volume fraction of banana and bagasse fibers of equal proportions are taken for fabrication using hand lay-up method for the dimension 300 * 300 mm2. Change in the volume fraction of FRC’s changes the value of young’s modulus by making the material more stiffer. The testing was carried out by the computer-integrated universal testing machine (UTM), which has the capacity of 100KN Kalpak software is used for the data acquisition the testing. The specimen is cut into as per ASTM D-3039 and ASTM D-790 standards for tensile test and flexural bending test, respectively. Tensile test and flexural bending tests are conducted on 20 and 30% of volume fraction of fibers (banana and bagasse). Results of tensile test and flexural bending test are obtained experimentally and compared each other, results reveal that fibers of 30% volume fraction make material stiffer and in turn increases the elasticity and UTS.

Secure Internet-of- Things: Assessing Challenges and Scopes for NextGen Communication

Abstract: The exponential rises in wireless communication technologies and allied application demands have revitalized academia-industries to achieve more efficient Quality of Service (QoS) centric communication solutions. To achieve it, in the last few years Internet-of-Things (IoT) technology has emerged as a potential solution. However, ensuring QoS provision and reliable communication across the network has always been a challenge for research community. Being a highly interfaced and complex paradigm IoT requires assuring secure communication across the network to achieve QoS and reliable data transmission. Assuring end-to-end security in IoT has revitalized academia-industries to develop more effective solution by developing advanced cryptosystem methods; however maintaining lightweight computation, seamless communication and QoS delivery simultaneously has been a driving force behind numerous ongoing researches. With this motive, in this paper emphasis is made on studying and assessing different security challenges in IoT ecosystem, solutions proposed so far and scopes for further optimization so as to develop a more effective IoT security system. The survey paper revealed that though numerous efforts have been made to augment IoT communication security, majority of the classical approaches either undergoes high computational overheads or impacts QoS delivery with real-time network solution. Additionally, it reveals that most of the existing systems focus on using single cryptosystem for communication security; however it fails alleviating different attack scenarios with different breaching actions. Majority of the existing approaches are found confined to address the security-vulnerabilities such asSmart Card Loss Attack (SCLA), offline password guessing using Brute Force attack, sensor node spoofing, privileged-insider and Session specific temporary information attack (SSTIA), etc. It suggests designing a robust security model with lightweight cryptosystem armored with multilevel security and authentication system.

"Prevention of structural perturbation and aggregation of hepatitis B surface antigen: screening of various additives".

Abstract: The instability of protein and antigen(s) during encapsulation in biodegradable polymers by water-in-oil-in-water (w/o/w) encapsulation is well established. The aim of present study is to screen various additives to prevent the inactivation and loss of immunogenicity of HBsAg upon its exposure to the water/CH(2)Cl(2) (methylene chloride) interface by simulating the formulation steps involved in the preparation of microspheres. The secondary structure of HBsAg, recovered under different conditions after primary emulsification, was investigated by FTIR spectroscopy and Circular Dichorism. Subsequently, PLGA microspheres were formulated and characterized for their size, shape, incorporation efficiency, antigen integrity, and immunogenicity. The immunogenicity and the HBsAg recovery under different conditions were tested in BALB/c mice. Inulin and trehalose were found to be better stabilizing agents to prevent the aggregation, the structural perturbations and immunogenicity of HBsAg. This study substantiated that inulin could overcome the aggregation and denaturing effects of the water/CH(2)Cl(2) interface upon HBsAg during emulsification step and upon encapsulation.