Dr. Hari Singh Gour University

About: Dr. Hari Singh Gour University is a education organization based out in Saugor, Madhya Pradesh, India. It is known for research contribution in the topics: Drug delivery & Computer science. The organization has 1120 authors who have published 1315 publications receiving 29511 citations. The organization is also known as: Dr Harisingh Gour Vishwavidyalaya & Sagar University.

Antioxidant, antiproliferative and antihemolytic properties of phytofabricated silver nanoparticles using Simarouba glauca and Celastrus paniculatus extracts

Abstract: The present research work depicts a cinch and economical synthesis of silver nanoparticles (AgNPs) phytofabricated utilizing leaf extract of Simarouba glauca and aerial extract of Celastrus paniculatus. Fourier transform infrared spectroscopy (FTIR) analysis displayed the synthesized AgNPs capped with bio-constituents existent in these medicinal plants marked by their functional groups. The morphological features of prepared NPs were characterized by employing X-ray Diffraction (XRD), Scanning Electron Microscope with Energy Dispersive spectra (SEM–EDX) and Transmission Electron Microscope (TEM) analysis illustrated their spherical shape and ~ 40 nm particle size. Green synthesized AgNPs were assessed for their antioxidant potential by free radical scavenging assays; and antibacterial efficacy. The phytofabricated AgNPs were found to induce cell growth arrest in MCF-7 and HT-29 cell lines with an IC50 value of 70.84 ± 0.67 and 158.24 ± 0.89 µg/mL for AgNPs prepared using S. glauca (Ag-SG) while 207.19 ± 0.64 and 221.22 ± 0.57 µg/mL for AgNPs synthesized using C. paniculatus (Ag-CP) in dose-proportional relation. The same samples could not effectively prohibit the growth of immortalized normal human breast epithelial cell lines (MCF-10A). The cytotoxicity induced by apoptosis was further confirmed by fluorescent images. The synthesized nanoparticles also demonstrated less hemolysis efficiency and are evidenced by SEM images. The analyses indicate the potent antioxidant and antiproliferative capacity of stable green synthesized AgNPs derived from the mentioned exorbitant curative herbs.

New Constituent from the Roots of Terminalia arjuna: Antifungal Agent.

Abstract: Ev id ences are presented for th e stru cture of a new compound , 2a., 19a.-di hydroxy-3-oxo-olean-12-en-28-oic acid- 28-0-~-D-glucopyranoside which occurs together with known substances ~-amyrin and friedelin in the roots of Termillalia (lrjl/ ll a. Biological screening of the compoll nd reveal antifungal ac ti vity .

Growth Inhibitory Effects of Adhatoda vasica and Its Potential at Reducing Listeria monocytogenes in Chicken Meat.

Abstract: The inhibitory effects of Adhatoda vasica ethanolic leaf extract (AVELE) were examined against Listeria monocytogenes, in order to assess its potential to preserve minimally processed meat products safely. Total phenolic, flavonoid, and alkaloid levels in AVELE were 10.09 ± 4.52 mg of gallic acid equivalents (GAE)/g, 22.43 ± 1.62 mg of quercetin equivalents/g, and 19.43 ± 3.90 mg/g, respectively. AVELE (at 1, 5, 10 or 20%) exhibited considerable antibacterial effects against L. monocytogenes; inhibitory zones of 7.4 to 13.6 mm, MIC of 100 mg/mL (10% formulated solution), reduced cell viability, potassium ion efflux, excess release of 260-nm absorbing materials and extracellular ATP. AVELE was used as a rinse solution (at 5, 10 and 20%) for raw chicken breast meat, and a 20% rinsing solution applied for 60 min significantly inhibited L. monocytogenes on raw chicken breast meat, and when the rinse time was increased to 90 min at the same concentration, L. monocytogenes did not grow in meat samples. L. monocytogenes was reduced to ~3 CFU/g after rinsing with 20% or 10% AVELE solution for 30 min. This work demonstrates the effectiveness of AVELE against L. monocytogenes, and suggests AVELE could be used as an effective antimicrobial marinade and rinse on meat products.

Synthesis and Biological Significance of 2‐Mercaptobenzoxazole Derivatives.

Abstract: Several 2-aryl-3-[(2-mercaptobenzoxazolo)acetamidyl]-4-oxothiazolidines 4, and 2-aryl-3-[(2-mercaptobenzoxazolo)acetamidyl]-5-arylidene-4-oxothiazolidines 5 have been synthesized by the appropriate methods and evaluated for their antibacterial activity against Escherichia coli, Shigella dysenteriae, Streptococcus aureus and Salmonella typhimurium; antifungal activity against Aspergillus niger, Candida albicans, Fusarium heterosporium and Rizopus oryzae and antiinflammatory activity against the carrageenan induced rat paw oedema method in albino rats. In the primary screening some of the products displayed acceptable biological activity. The structure of the synthesized compounds has been established on the basis of their spectral and microanalytical data.