Dr. Hari Singh Gour University

About: Dr. Hari Singh Gour University is a education organization based out in Saugor, Madhya Pradesh, India. It is known for research contribution in the topics: Drug delivery & Computer science. The organization has 1120 authors who have published 1315 publications receiving 29511 citations. The organization is also known as: Dr Harisingh Gour Vishwavidyalaya & Sagar University.

Inhibition of Group IIA Secretory Phospholipase A2 and its Inflammatory Reactions in Mice by Ethanolic Extract of Andrographis paniculata, a Well-known Medicinal Food.

Abstract: Andrographis paniculata Nees is an important medicinal plant found in the tropical regions of the world, which has been traditionally used in Indian and Chinese medicinal systems. It is also used as medicinal food. A. paniculata is found to exhibit anti-inflammatory activities; however, its inhibitory potential on inflammatory Group IIA phospholipases A 2 (PLA 2 ) and its associated inflammatory reactions are not clearly understood. The aim of the present study is to evaluate the inhibitory/neutralizing potential of ethanolic extract of A. paniculata on the isolated inflammatory PLA 2 (VRV-PL-VIIIa) from Daboii rusellii pulchella (belonging to Group IIA inflammatory secretory PLA 2 [sPLA 2 ]) and its associated edema-induced activities in Swiss albino mice. A. paniculata extract dose dependently inhibited the Group IIA sPLA 2 enzymatic activity with an IC 50 value of 10.3 ± 0.5 μg/ml. Further, the extract dose dependently inhibited the edema formation, when co-injected with enzyme indicating that a strong correlation exists between lipolytic and pro-inflammatory activities of the enzyme. In conclusion, results of this study shows that the ethanolic extract of A. paniculata effectively inhibits Group IIA sPLA 2 and its associated inflammatory activities, which substantiate its anti-inflammatory properties. The results of the present study warranted further studies to develop bioactive compound (s) in ethanolic extract of A. paniculata as potent therapeutic agent (s) for inflammatory diseases. SUMMARY This study emphasis the anti-inflammatory effect of A. paniculata by inhibiting the inflammatory Group IIA sPLA2 and its associated inflammatory activities such as edema. It was found that there is a strong correlation between lipolytic activity and pro-inflammatory activity inhibition. Therefore, the study suggests that the extract processes potent anti-inflammatory agents, which could be developed as a potential therapeutic agent against inflammatory and related diseases.

Poloxamer-coated three-ply-walled microcapsules for controlled delivery of diclofenac sodium.

Abstract: The three-ply-walled-based system for controlled delivery of diclofenac was developed. The preparation of three-ply-walled microcapsules is essentially based on the technique of multiple-emulsion formation polymer at the interface followed by rigidization of the wall on evaporation of solvent. The protective colloids with surface-active properties were selected for the present study, viz. acacia gelatin, polyvinyl alcohol and sodium alginate. Ethyl cellulose was taken as hydrophobic polymer. The acacia/ethylcellulose/acacia-based three-ply-walled microcapsule system was selected for further studies. The three-ply-walled microcapsule were subsequently coated with poloxamer 188. The non-ionic hydrophilic surfactant coating retards uptake into the reticuloendothelial system. The coated and uncoated microcapsules were characterized for in vitro and in vivo performance. The microcapsules were noted to provide sustained release of the contained diclofenac. The plasma level observed indicated that poloxa...

Chemosterilant activity of naturally occurring quinones and their analogues in the red cotton bug, Dysdercus koenigii (Het., Pyrrhocoridae)

Abstract: The chemosterilant activity of two naturally occurring napthaquinones, plumbagin from Plumbago sp. and juglone from Juglans regia has been evaluated using the red cotton bug Dysdercus koenigii. Their activity were compared with synthetic napthaquinones, menadione, two benzoquinones, 2,6-dimethyl and 2,3,6-trimethylbenzoquinone and a hydroquinone 2,6-dimethylhydroquinone. As far as the authors are aware, the present investigation is the first systematic attempt to investigate the effects of quinones on different aspects of reproduction, namely mating behaviour, fecundity and fertility. All the above types of quinones have revealed adverse effects on the reproduction of D. koenigii, which were topically treated as 1-day-old adults with different doses of the compounds depending on their 50% lethality (LD 50 ) values. Analysis of the data revealed a highly statistically significant reduction in the number of eggs, their hatching and further development up to the final instar stage. The sterility index increased with the increase in the dose of any of the above compounds and was highest when both sexes were treated. Among the natural products, juglone induced more sterilizing effects than plumbagin at 5-10 μg/insect. Menadione caused sterility at a very low dose such as 0.5 μg. Of the two benzoquinones, the dimethylbenzoquinone acted at a much lower dose.