Dr. Hari Singh Gour University

About: Dr. Hari Singh Gour University is a education organization based out in Saugor, Madhya Pradesh, India. It is known for research contribution in the topics: Drug delivery & Computer science. The organization has 1120 authors who have published 1315 publications receiving 29511 citations. The organization is also known as: Dr Harisingh Gour Vishwavidyalaya & Sagar University.

“Immunoporosis”: Immunology of Osteoporosis

Abstract: Bone is a dynamic organ that maintains its proper architecture and function by undergoing continuous cycles of modelling and remodelling during normal host physiology. Any dysregulation in the bone remodelling process leads to the development of bone-related disorders including osteoporosis. Osteoporosis is considered as one of the most commonly occurring but often neglected bone diseases leading to enhanced fragility and morbidity in large number of populations. Presently, available drugs (strontium, bisphosphonates, oestrogen replacement therapy, etc.) that are being used as a therapy for treating osteoporosis come with higher side effects and complications later in life. Thus, newer therapies are need of the hour for better management and treatment of osteoporosis-related bone loss. Various studies indicate that immune responses (both innate and adaptive) play crucial role in the development and maintenance of inflammatory conditions including osteoporosis. Thus, in present review we specifically discuss about the role of innate (DCs, NK cells, macrophages and ILCs) and adaptive (Th1, Th2, Th17, Tregs and B cells) immune cells in progression of osteoporosis. This novel field recently proposed and coined as “Immunoporosis” (Immunology of Osteoporosis) by our group, deals with the cross talk between immune and bone cells providing excellent dedicated interface for understanding the complexity of these two unique systems in osteoporosis with potential for providing novel molecular insights in the development of effective therapeutics for osteoporosis.

In Silico Computational Screening of Kabasura Kudineer - Official Siddha Formulation and JACOM - Novel Herbal Coded Formulation Against SARS-CoV-2 Spike protein

Abstract: Siddha Medicine is a valuable therapeutic choice which is classically used for treating viral respiratory infections, this principle of medicine is proven to contain antiviral compounds. In this study, we executed the molecular docking studies for Phytoconstituents of Siddha official formulation Kabasura Kudineer which is used in treating viral fever and respiratory diseases and a Novel Herbal Preparation - JACOM against the ongoing pandemic of novel coronavirus disease causative agent SARS-CoV-2 Spike protein. Further, we also conducted prediction studies on the pharmacokinetics (ADME) properties and the safety profile in order to identify the best drug candidates using pkCSM and SwissADME web servers. Totally 37 compounds were screened, of these 9 compounds showed high binding efficiency. Based on these, we proposed the new formulation called as “SNACK –V”.

Phytogenic synthesis of silver nanobactericides for anti-biofilm activity against human pathogen H. pylori

Abstract: The present study reports the phytogenic synthesis of silver nanobactericides using Acorus calamus L. and their anti-biofilm activity against clinically isolated H. pylori. The synthesis was confirmed with change in the color of the reaction mixture to brown. The increased in the color intensity was periodically monitored with UV–visible spectroscopy which displayed maximum absorption at 410 nm. The biomolecular interaction was studied with FTIR spectral measurements of silver nanobactericides which revealed the presence of broad absorbance band appearing at 3361 is due to OH group and the prominent peak at 1634 correspond to an amide group. X-ray diffraction (XRD) displayed Bragg’s intensities at 2θ angle reflecting (111), (200), (220) and (311) of the face centered cubic (fcc) structure of silver which was compared with standard XRD pattern. The morphological characteristics of nanobactericides were studied using Transmission electron microscopy (TEM) analysis which revealed the polydispersity of nanoparticles with size ranging from 5 to 60 nm. The anti-biofilm activity of silver nanobactericides against H. pylori was measured using crystal violet and ruthenium red assays which revealed 350 µg/mL to be more effective. The obtained activity was validated with standard antibiotics amoxicillin. Overall, the results obtained in the present investigation are promising enough to reveal the efficacy of silver nanoparticles to inhibit the biofilm production.

Predicting anti-HIV activity of 1,1,3-trioxo[1,2,4]-thiadiazine (TTD) derivatives: 3D QSAR approach

Abstract: Human immunodeficiency virus type 1 (HIV-1) is the pathogenic retrovirus and causative agent of acquired immunodeficiency syndrome (AIDS) HIV-1 reverse transcriptase (RT) is one of the key enzymes in the duplication of HIV-1 Inhibitors of HIV-1 RT are classified as nonnucleoside reverse-transcriptase inhibitors (NNRTIs) and nucleoside reverse-transcriptase inhibitors (NRTIs) NNRTIs bind in a region not associated with the active site of the enzyme Within the NNRTIs category, there is a set of inhibitors commonly referred to as 1,1,3-trioxo-thiadiazine (TTD) derivatives Based on the structures and biodata of previous TDS analogs, three-dimensional (3D) quantitative structure–activity relationship (QSAR) studies have been performed which resulted in two reliable computational models, comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA) with r 2 values of 0883 and 0852 and q 2 values of 0639 and 0608, respectively The predictive ability of the developed CoMFA and CoMSIA models was also confirmed by using a test (external validation) set comprised of eight molecules, with predicted r 2 value of 0832 and 0760, respectively It is shown that the steric and electrostatic properties predicted by CoMFA contours and hydrophobic properties predicted by CoMSIA contours can be related to anti-HIV activity These models are a significant guide to trace the features that really matter, especially with respect to design of novel compounds Comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA) of 1,1,3-trioxo-thiadiazine (TTDs) derivatives The parameters indicated are responsible for increasing anti-HIV activity