Institution
Environmental Molecular Sciences Laboratory
Facility•Richland, Washington, United States•
About: Environmental Molecular Sciences Laboratory is a facility organization based out in Richland, Washington, United States. It is known for research contribution in the topics: Mass spectrometry & Ion. The organization has 1471 authors who have published 3010 publications receiving 169961 citations.
Topics: Mass spectrometry, Ion, X-ray photoelectron spectroscopy, Catalysis, Fourier transform ion cyclotron resonance
Papers published on a yearly basis
Papers
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TL;DR: Development of chassis-independent recombinase-assisted genome engineering (CRAGE) enables the integration of plasmids encoding biosynthetic gene clusters into the chromosomes of diverse bacteria to optimize production of natural products in non-native strains.
Abstract: It is generally believed that exchange of secondary metabolite biosynthetic gene clusters (BGCs) among closely related bacteria is an important driver of BGC evolution and diversification. Applying this idea may help researchers efficiently connect many BGCs to their products and characterize the products' roles in various environments. However, existing genetic tools support only a small fraction of these efforts. Here, we present the development of chassis-independent recombinase-assisted genome engineering (CRAGE), which enables single-step integration of large, complex BGC constructs directly into the chromosomes of diverse bacteria with high accuracy and efficiency. To demonstrate the efficacy of CRAGE, we expressed three known and six previously identified but experimentally elusive non-ribosomal peptide synthetase (NRPS) and NRPS-polyketide synthase (PKS) hybrid BGCs from Photorhabdus luminescens in 25 diverse γ-Proteobacteria species. Successful activation of six BGCs identified 22 products for which diversity and yield were greater when the BGCs were expressed in strains closely related to the native strain than when they were expressed in either native or more distantly related strains. Activation of these BGCs demonstrates the feasibility of exploiting their underlying catalytic activity and plasticity, and provides evidence that systematic approaches based on CRAGE will be useful for discovering and identifying previously uncharacterized metabolites.
71 citations
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TL;DR: A global strategy that aims to extend the sensitivity, dynamic range, comprehensiveness, and throughput of proteomic measurements for microbial systems based upon the use of polypeptide accurate mass tags produced by global protein enzymatic digestions by exploiting high accuracy mass measurements using FTICR.
Abstract: We describe and review progress towards a global strategy that aims to extend the sensitivity, dynamic range, comprehensiveness, and throughput of proteomic measurements for microbial systems based upon the use of polypeptide accurate mass tags (AMTs) produced by global protein enzymatic digestions. The two-stage strategy exploits high accuracy mass measurements using Fourier transform ion cyclotron resonance mass spectrometry (FTICR) to validate polypeptide AMTs for a specific organism, from potential mass tags tentatively identified using tandem mass spectrometry (MS/MS), providing the basis for subsequent measurements without the need for routine MS/MS. A high-resolution capillary liquid chromatography separation combined with high sensitivity, and high-resolution accurate FTICR measurements is shown to be capable of characterizing polypeptide mixtures of more than 105 components, sufficient for broad protein identification using AMTs. Advantages of the approach include the high confidence of protein i...
71 citations
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TL;DR: In this paper, the effect of thermal treatment on the structure and chemical properties of Ba-oxide-based NO x storage/reduction catalysts with different Ba loadings was investigated using BET, TEM, EDS, TPD and FT-IR techniques.
71 citations
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TL;DR: In this article, the fate of carbon stored in permafrost-zone peatlands represents a significant uncertainty in global climate modeling, given that the breakdown of dissolved organic matter (DOM) is often a major p...
71 citations
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TL;DR: In this article, a cost-effective hydrothermal carbonization approach was used to prepare a hard carbon coated nano-Si/graphite (HC-nSi/G) composite as a high performance anode for Li-ion batteries.
71 citations
Authors
Showing all 1477 results
Name | H-index | Papers | Citations |
---|---|---|---|
George M. Whitesides | 240 | 1739 | 269833 |
Yi Cui | 220 | 1015 | 199725 |
Donald G. Truhlar | 165 | 1518 | 157965 |
Ronald W. Davis | 155 | 644 | 151276 |
Richard D. Smith | 140 | 1180 | 79758 |
Yuehe Lin | 118 | 641 | 55399 |
Robert C. Haddon | 112 | 577 | 52712 |
Lai-Sheng Wang | 103 | 576 | 36212 |
Mark H. Engelhard | 103 | 545 | 39864 |
Alex Guenther | 100 | 447 | 45476 |
Gordon E. Brown | 100 | 454 | 32152 |
X. Sunney Xie | 98 | 225 | 44104 |
Jun Li | 98 | 631 | 40958 |
Richard A. Friesner | 97 | 367 | 52729 |
Chongmin Wang | 95 | 451 | 33983 |