Journal ArticleDOI
A clonogenic common myeloid progenitor that gives rise to all myeloid lineages
TLDR
The prospective identification, purification and characterization, using cell-surface markers and flow cytometry, of a complementary clonogenic common myeloid progenitor that gives rise to all myeloids lineages is reported.Abstract:
Haematopoietic stem cells give rise to progeny that progressively lose self-renewal capacity and become restricted to one lineage. The points at which haematopoietic stem cell-derived progenitors commit to each of the various lineages remain mostly unknown. We have identified a clonogenic common lymphoid progenitor that can differentiate into T, B and natural killer cells but not myeloid cells. Here we report the prospective identification, purification and characterization, using cell-surface markers and flow cytometry, of a complementary clonogenic common myeloid progenitor that gives rise to all myeloid lineages. Common myeloid progenitors give rise to either megakaryocyte/erythrocyte or granulocyte/macrophage progenitors. Purified progenitors were used to provide a first-pass expression profile of various haematopoiesis-related genes. We propose that the common lymphoid progenitor and common myeloid progenitor populations reflect the earliest branch points between the lymphoid and myeloid lineages, and that the commitment of common myeloid progenitors to either the megakaryocyte/erythrocyte or the granulocyte/macrophage lineages are mutually exclusive events.read more
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Journal ArticleDOI
Identification of the haematopoietic stem cell niche and control of the niche size
Jiwang Zhang,Chao Niu,Ling Ye,Haiyang Huang,Xi C. He,Wei Gang Tong,Jason Ross,Jeffrey S. Haug,Teri Johnson,Jian Q. Feng,Stephen E. Harris,Leanne M. Wiedemann,Leanne M. Wiedemann,Yuji Mishina,Linheng Li,Linheng Li +15 more
TL;DR: It is concluded that SNO cells lining the bone surface function as a key component of the niche to support HSCs, and that BMP signalling through BMPRIA controls the number of H SCs by regulating niche size.
Journal ArticleDOI
Direct isolation of human central nervous system stem cells
Nobuko Uchida,David W. Buck,Dongping He,Michael J. Reitsma,Marilyn Masek,Thinh V. Phan,Ann Tsukamoto,Fred H. Gage,Irving L. Weissman +8 more
TL;DR: Upon transplantation into brains of immunodeficient neonatal mice, the sorted/expanded hCNS-SC showed potent engraftment, proliferation, migration, and neural differentiation.
Journal ArticleDOI
Blood monocytes: development, heterogeneity, and relationship with dendritic cells.
TL;DR: Functional characterization of monocytes is in progress in humans and rodents and will provide a better understanding of the pathophysiology of inflammation.
Journal ArticleDOI
Granulocyte-Macrophage Progenitors as Candidate Leukemic Stem Cells in Blast-Crisis CML
Catriona Jamieson,Laurie Ailles,Scott J. Dylla,Manja Muijtjens,Carol D. Jones,James L. Zehnder,Jason Gotlib,Kevin Li,Markus G. Manz,Armand Keating,Charles L. Sawyers,Irving L. Weissman +11 more
TL;DR: Activation of beta-catenin in CML granulocyte-macrophage progenitors appears to enhance the self-renewal activity and leukemic potential of these cells.
Journal ArticleDOI
FoxOs are critical mediators of hematopoietic stem cell resistance to physiologic oxidative stress.
Zuzana Tothova,Zuzana Tothova,Ramya Kollipara,Brian J. P. Huntly,Benjamin H. Lee,Benjamin H. Lee,Diego H. Castrillon,Dana E. Cullen,Dana E. Cullen,Elizabeth P. McDowell,Elizabeth P. McDowell,Suzan Lazo-Kallanian,Ifor R. Williams,Christopher Sears,Scott A. Armstrong,Emmanuelle Passegué,Ronald A. DePinho,D. Gary Gilliland,D. Gary Gilliland +18 more
TL;DR: FoxO proteins play essential roles in the response to physiologic oxidative stress and thereby mediate quiescence and enhanced survival in the HSC compartment, a function that is required for its long-term regenerative potential.
References
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Journal ArticleDOI
Purification and Characterization of Mouse Hematopoietic Stem Cells
TL;DR: Mouse bone marrow hematopoietic stem cells were isolated with the use of a variety of phenotypic markers and thirty of these cells are sufficient to save 50 percent of lethally irradiated mice, and to reconstitute all blood cell types in the survivors.
Journal ArticleDOI
Identification of Clonogenic Common Lymphoid Progenitors in Mouse Bone Marrow
TL;DR: The Lin(-)IL-7R(+)Thy-1(-)Sca-1loc-Kit(lo) population from adult mouse bone marrow possessed a rapid lymphoid-restricted (T, B, and NK) reconstitution capacity in vivo but completely lacked myeloid differentiation potential either in vivo or in vitro.
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Long-Term Lymphohematopoietic Reconstitution by a Single CD34-Low/Negative Hematopoietic Stem Cell
TL;DR: A monoclonal antibody raised to the mouse homolog of CD34 (mCD34) was used to purify mouse HSCs to near homogeneity to enable analysis of the self-renewal and multilineage differentiation of individual HSCS.
Journal ArticleDOI
Differentiation and Proliferation of Hematopoietic Stem Cells
TL;DR: This simple model fits the understanding of the interactions of growth factors with hematopoietic progenitors and risks oversimplification of a very complex process.
Journal ArticleDOI
Erythroid differentiation in chimaeric mice blocked by a targeted mutation in the gene for transcription factor GATA-1
Larysa Pevny,M. Celeste Simon,Elizabeth J. Robertson,William H. Klein,Shih-Feng Tsai,Vivette D. D'Agati,Stuart H. Orkin,Frank Costantini +7 more
TL;DR: The disruption of the X-linked GATA-1 gene by homologous recombination in a male (XY) murine embryonic stem cell line and testing the Gata-1-deficient cells for their ability to contribute to different tissues in chimaeric mice demonstrates that GATA, the zinc-finger transcription factor, is required for the normal differentiation of erythroid cells, and that other GATAS cannot compensate for its absence.