A diet high in resistant starch modulates microbiota composition, SCFA concentrations, and gene expression in pig intestine
Daniëlle Haenen,Jing Zhang,Carol Souza da Silva,Guido Bosch,Ingrid M. van der Meer,Jeroen van Arkel,Joost J. G. C. van den Borne,Odette N. Pérez Gutiérrez,Hauke Smidt,Bas Kemp,Michael Müller,Guido J. E. J. Hooiveld +11 more
Reads0
Chats0
TLDR
Investigation of the effects of a diet high in RS on luminal microbiota composition, luminal SCFA concentrations, and the expression of host genes involved in SCFA uptake, SCFA signaling, and satiety regulation in mucosal tissue obtained from small intestine, cecum, and colon shows that RS modulates microbiota compositionAbstract:
Resistant starch (RS) is highly fermentable by microbiota in the colon, resulting in the production of SCFAs. RS is thought to mediate a large proportion of its health benefits, including increased satiety, through the actions of SCFAs. The aim of this study was to investigate the effects of a diet high in RS on luminal microbiota composition, luminal SCFA concentrations, and the expression of host genes involved in SCFA uptake, SCFA signaling, and satiety regulation in mucosal tissue obtained from small intestine, cecum, and colon. Twenty adult female pigs were either assigned to a digestible starch (DS) diet or a diet high in RS (34%) for a period of 2 wk. After the intervention, luminal content and mucosal scrapings were obtained for detailed molecular analysis. RS was completely degraded in the cecum. In both the cecum and colon, differences in microbiota composition were observed between DS- and RS-fed pigs. In the colon these included the stimulation of the healthy gut-associated butyrate-producing Faecalibacterium prausnitzii, whereas potentially pathogenic members of the Gammaproteobacteria, including Escherichia coli and Pseudomonas spp., were reduced in relative abundance. Cecal and colonic SCFA concentrations were significantly greater in RS-fed pigs, and cecal gene expression of monocarboxylate transporter 1 (SLC16A1) and glucagon (GCG) was induced by RS. In conclusion, our data show that RS modulates microbiota composition, SCFA concentrations, and host gene expression in pig intestine. Combined, our data provide an enhanced understanding of the interaction between diet, microbiota, and host.read more
Citations
More filters
Journal ArticleDOI
The role of short-chain fatty acids in the interplay between diet, gut microbiota, and host energy metabolism
Gijs den Besten,Karen van Eunen,Albert K. Groen,Koen Venema,Dirk-Jan Reijngoud,Barbara M. Bakker +5 more
TL;DR: This review summarizes the role of SCFAs in host energy metabolism, starting from the production by the gut microbiota to the uptake by the host and ending with the effects on host metabolism.
Journal ArticleDOI
Short Chain Fatty Acids (SCFAs)-Mediated Gut Epithelial and Immune Regulation and Its Relevance for Inflammatory Bowel Diseases.
Daniela Parada Venegas,Marjorie De la Fuente,Glauben Landskron,María-Julieta González,Rodrigo Quera,Gerard Dijkstra,Hermie J. M. Harmsen,Klaas Nico Faber,Marcela A. Hermoso +8 more
TL;DR: An overview of microbial SCFAs production and their effects on the intestinal mucosa with specific emphasis on their relevance for Inflammatory Bowel Diseases is presented and the therapeutic potential ofSCFAs for IBD is discussed.
Journal ArticleDOI
Butyrate: A Double-Edged Sword for Health?
TL;DR: The present knowledge on the properties of butyrate, especially its potential effects and mechanisms involved in intestinal health and obesity, are summarized.
Journal ArticleDOI
A Perspective on the Complexity of Dietary Fiber Structures and Their Potential Effect on the Gut Microbiota
Bruce R. Hamaker,Yunus E. Tuncil +1 more
TL;DR: A global framework needs to be developed to better understand how dietary fibers can be used to obtain predicted changes in microbiota composition for improved health and this will require a multi-disciplinary effort that includes biological scientists, clinicians, and carbohydrate specialists.
Journal ArticleDOI
Gut microbiome and aging: Physiological and mechanistic insights
Ravinder Nagpal,Rabina Mainali,Shokouh Ahmadi,Shokouh Ahmadi,Shaohua Wang,Ria Singh,Kylie Kavanagh,Dalane W. Kitzman,Almagul Kushugulova,Francesco Marotta,Hariom Yadav +10 more
TL;DR: The present review summarizes the evidences related to the age-associated changes in intestinal microbiota and vice-versa, mechanisms involved in this bi-directional relationship, and the prospective for development of microbiota-based interventions such as probiotics for healthy aging.
References
More filters
Journal ArticleDOI
Regulation of monocarboxylate transporter 1 (MCT1) promoter by butyrate in human intestinal epithelial cells: Involvement of NF‐κB pathway
Alip Borthakur,Seema Saksena,Ravinder K. Gill,Waddah A. Alrefai,Krishnamurthy Ramaswamy,Pradeep K. Dudeja +5 more
TL;DR: It is demonstrated that butyrate, a substrate for MCT1, stimulates M CT1 promoter activity in Caco‐2 cells and the involvement of NF‐κB pathway in the regulation of MCT 1 promoter activity bybutyrate is demonstrated.
Journal ArticleDOI
Absorption of carbohydrate-derived nutrients in sows as influenced by types and contents of dietary fiber.
TL;DR: The study showed that feeding the high dietary fiber diets resulted in a increased and more uniform uptake of SCFA than when feeding the LF control, and the HF-S diet reduced diurnal variation in glucose and insulin concentrations.
Journal ArticleDOI
Supplemental Dietary Inulin of Variable Chain Lengths Alters Intestinal Bacterial Populations in Young Pigs
TL;DR: The ability of dietary inulin to alter intestinal bacterial populations may partially account for its iron bioavailability-promoting effect and possibly other health benefits.
Journal ArticleDOI
New frontiers in gut nutrient sensor research: free fatty acid sensing in the gastrointestinal tract.
TL;DR: The physiological and pharmacological function of the receptors in the gastrointestinal tract ofFFAR1,FFAR2, FFAR3, and GPR120 are summarized.
Related Papers (5)
Short-Chain Fatty Acids and Human Colonic Function: Roles of Resistant Starch and Nonstarch Polysaccharides
QIIME allows analysis of high-throughput community sequencing data.
J. Gregory Caporaso,Justin Kuczynski,Jesse Stombaugh,Kyle Bittinger,Frederic D. Bushman,Elizabeth K. Costello,Noah Fierer,Antonio Gonzalez Peña,Julia K. Goodrich,Jeffrey I. Gordon,Gavin A. Huttley,Scott T. Kelley,Dan Knights,Jeremy E. Koenig,Ruth E. Ley,Catherine A. Lozupone,Daniel McDonald,Brian D. Muegge,Meg Pirrung,Jens Reeder,Joel Sevinsky,Peter J. Turnbaugh,William A. Walters,Jeremy Widmann,Tanya Yatsunenko,Jesse R. Zaneveld,Rob Knight,Rob Knight +27 more