A Population of Multipotent CD34-Positive Adipose Stromal Cells Share Pericyte and Mesenchymal Surface Markers, Reside in a Periendothelial Location, and Stabilize Endothelial Networks
Dmitry O. Traktuev,Stephanie Merfeld-Clauss,Jingling Li,Mikhail G. Kolonin,Wadih Arap,Renata Pasqualini,Brian H. Johnstone,Keith L. March,Keith L. March +8 more
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TLDR
The results demonstrate for the first time that the majority of adipose-derived adherent CD34+ cells are resident pericytes that play a role in vascular stabilization by mutual structural and functional interaction with endothelial cells.Abstract:
It has been shown that stromal–vascular fraction isolated from adipose tissues contains an abundance of CD34+ cells. Histological analysis of adipose tissue revealed that CD34+ cells are widely distributed among adipocytes and are predominantly associated with vascular structures. The majority of CD34+ cells from freshly isolated stromal–vascular fraction were CD31−/CD144− and could be separated from a distinct population of CD34+/CD31+/CD144+ (endothelial) cells by differential attachment on uncoated plastic. The localization of CD34+ cells within adipose tissue suggested that the nonendothelial population of these cells occupied a pericytic position. Analysis of surface and intracellular markers of the freshly isolated CD34+/CD31−/CD144− adipose-derived stromal cells (ASCs) showed that >90% coexpress mesenchymal (CD10, CD13, and CD90), pericytic (chondroitin sulfate proteoglycan, CD140a, and CD140b), and smooth muscle (α-actin, caldesmon, and calponin) markers. ASCs demonstrated polygonal self-assembly ...read more
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A perivascular origin for mesenchymal stem cells in multiple human organs
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CD8 + effector T cells contribute to macrophage recruitment and adipose tissue inflammation in obesity
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The MSC: An Injury Drugstore
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References
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Journal ArticleDOI
Human Adipose Tissue Is a Source of Multipotent Stem Cells
Patricia A. Zuk,Min Zhu,Peter Ashjian,Daniel A. De Ugarte,Jerry I. Huang,Hiroshi Mizuno,Zeni Alfonso,John K. Fraser,Prosper Benhaim,Marc H. Hedrick +9 more
TL;DR: To confirm whether adipose tissue contains stem cells, the PLA population and multiple clonal isolates were analyzed using several molecular and biochemical approaches and PLA cells exhibited unique characteristics distinct from those seen in MSCs, including differences in CD marker profile and gene expression.
Journal ArticleDOI
Mesenchymal stem cells reside in virtually all post-natal organs and tissues
TL;DR: The results suggest that the distribution of MSCs throughout the post-natal organism is related to their existence in a perivascular niche, which has implications for understanding MSC biology, and for clinical and pharmacological purposes.
Journal ArticleDOI
Transplantation of Progenitor Cells and Regeneration Enhancement in Acute Myocardial Infarction (TOPCARE-AMI)
Birgit Assmus,Volker Schächinger,Claudius Teupe,Martina B. Britten,Ralf Lehmann,N. Döbert,Frank Grünwald,Alexandra Aicher,Carmen Urbich,Hans Martin,Dieter Hoelzer,Stefanie Dimmeler,Andreas M. Zeiher +12 more
TL;DR: In patients with AMI, intracoronary infusion of autologous progenitor cells appears to be feasible and safe and may beneficially affect postinfarction remodeling processes.
Journal ArticleDOI
Secretion of Angiogenic and Antiapoptotic Factors by Human Adipose Stromal Cells
Jalees Rehman,Dmitry O. Traktuev,Jingling Li,Stephanie Merfeld-Clauss,Constance J. Temm-Grove,Jason E. Bovenkerk,Carrie L. Pell,Brian H. Johnstone,Robert V. Considine,Keith L. March +9 more
TL;DR: The findings suggest that autologous delivery of either native or transduced subcutaneous ASCs, which are regulated by hypoxia, may be a novel therapeutic option to enhance angiogenesis or achieve cardiovascular protection.
Journal ArticleDOI
Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood.
David A. Ingram,Laura E. Mead,Hiromi Tanaka,Virginia M. Meade,Amy Fenoglio,Kelly E. Mortell,Karen E. Pollok,Michael J. Ferkowicz,David Gilley,Mervin C. Yoder +9 more
TL;DR: These studies describe a clonogenic method to define a hierarchy of EPCs based on their proliferative potential, and they identify a unique population of high proliferation potential-endothelial colony-forming cells (HPP-ECFCs) in human umbilical cord blood.
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