Aldehyde dehydrogenase 1 is a tumor stem cell-associated marker in lung cancer.
Feng Jiang,Qi Qiu,Abha Khanna,Nevins W. Todd,Janaki Deepak,Lingxiao Xing,Huijun Wang,Zhenqiu Liu,Yun Su,Sanford A. Stass,Ruth L. Katz +10 more
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TLDR
Immunohistochemical analysis of 303 clinical specimens from three independent cohorts of lung cancer patients and controls show that expression of ALDH1 is positively correlated with the stage and grade of lung tumors and related to a poor prognosis for the patients with early-stage lung cancer.Abstract:
Tumor contains small population of cancer stem cells (CSC) that are responsible for its maintenance and relapse. Analysis of these CSCs may lead to effective prognostic and therapeutic strategies for the treatment of cancer patients. We report here the identification of CSCs from human lung cancer cells using Aldefluor assay followed by fluorescence-activated cell sorting analysis. Isolated cancer cells with relatively high aldehyde dehydrogenase 1 (ALDH1) activity display in vitro features of CSCs, including capacities for proliferation, self-renewal, and differentiation, resistance to chemotherapy, and expressing CSC surface marker CD133. In vivo experiments show that the ALDH1-positive cells could generate tumors that recapitulate the heterogeneity of the parental cancer cells. Immunohistochemical analysis of 303 clinical specimens from three independent cohorts of lung cancer patients and controls show that expression of ALDH1 is positively correlated with the stage and grade of lung tumors and related to a poor prognosis for the patients with early-stage lung cancer. ALDH1 is therefore a lung tumor stem cell-associated marker. These findings offer an important new tool for the study of lung CSCs and provide a potential prognostic factor and therapeutic target for treatment of the patients with lung cancer. (Mol Cancer Res 2009;7(3):330–8)read more
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Journal ArticleDOI
The Multifaceted Role of Aldehyde Dehydrogenases in Prostate Cancer Stem Cells.
TL;DR: In this article, the authors discuss the role of individual ALDH isoforms in prostate cancer development and progression, their possible therapeutic targeting, and provide an outlook for the future advances in this field.
Journal ArticleDOI
High PKCλ expression is required for ALDH1-positive cancer stem cell function and indicates a poor clinical outcome in late-stage breast cancer patients.
Yuka Nozaki,Hitomi Motomura,Shoma Tamori,Yumiko Kimura,Chotaro Onaga,Shotaro Kanai,Yuka Ishihara,Ayaka Ozaki,Yasushi Hara,Yohsuke Harada,Yasunari Mano,Tsugumichi Sato,Keiko Sato,Kazunori Sasaki,Hitoshi Ishiguro,Shigeo Ohno,Kazunori Akimoto +16 more
TL;DR: It is suggested that PKCλ is essential for cancer cell survival and migration, tumorigenesis, the asymmetric distribution of ALDH1A3 protein among cancer cells, and the maintenance of low ROS levels in ALDH2-positive breast cancer stem cells, which makes it a key contributor to the poorer prognosis seen in late stage breast cancer patients.
Journal ArticleDOI
Immunohistochemical and genetic characteristics of lung cancer mimicking organizing pneumonia.
Tomohiro Ichikawa,Koichi Saruwatari,Sachiyo Mimaki,Masato Sugano,Keiju Aokage,Motohiro Kojima,Tomoyuki Hishida,Satoshi Fujii,Junji Yoshida,Takeshi Kuwata,Atsushi Ochiai,Kenji Suzuki,Masahiro Tsuboi,Koichi Goto,Katsuya Tsuchihara,Genichiro Ishii +15 more
TL;DR: The results indicate that LCOP is characterized by fewer tumor-promoting stromal cells, which may contribute to the better prognosis of LCOP patients.
Journal ArticleDOI
Detection of ALDH1 activity in rabbit hepatic VX2 tumors and isolation of ALDH1 positive cancer stem cells
TL;DR: The overall results of this study indicate that ALDH1 is a valid CSC marker for VX2 cancer, which suggests that the rabbit V X2 liver cancer model is useful in studying drug resistance in hepatocellular carcinoma and may be useful for basic and preclinical studies of other types of human cancer.
Book ChapterDOI
The Role of ALDH in the Metastatic Potential of Osteosarcoma Cells and Potential ALDH Targets.
Rebekah Belayneh,Kurt R. Weiss +1 more
TL;DR: A more complete understanding of aldehyde dehydrogenase in osteosarcoma will give a deeper knowledge of its impact on osteosArcoma metastatic potential, and it is hoped that this knowledge can be translated into novel antimetastatic therapeutic strategies and thus improve osteosARcoma prognoses.
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Journal ArticleDOI
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