Aldehyde dehydrogenase 1 is a tumor stem cell-associated marker in lung cancer.
Feng Jiang,Qi Qiu,Abha Khanna,Nevins W. Todd,Janaki Deepak,Lingxiao Xing,Huijun Wang,Zhenqiu Liu,Yun Su,Sanford A. Stass,Ruth L. Katz +10 more
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TLDR
Immunohistochemical analysis of 303 clinical specimens from three independent cohorts of lung cancer patients and controls show that expression of ALDH1 is positively correlated with the stage and grade of lung tumors and related to a poor prognosis for the patients with early-stage lung cancer.Abstract:
Tumor contains small population of cancer stem cells (CSC) that are responsible for its maintenance and relapse. Analysis of these CSCs may lead to effective prognostic and therapeutic strategies for the treatment of cancer patients. We report here the identification of CSCs from human lung cancer cells using Aldefluor assay followed by fluorescence-activated cell sorting analysis. Isolated cancer cells with relatively high aldehyde dehydrogenase 1 (ALDH1) activity display in vitro features of CSCs, including capacities for proliferation, self-renewal, and differentiation, resistance to chemotherapy, and expressing CSC surface marker CD133. In vivo experiments show that the ALDH1-positive cells could generate tumors that recapitulate the heterogeneity of the parental cancer cells. Immunohistochemical analysis of 303 clinical specimens from three independent cohorts of lung cancer patients and controls show that expression of ALDH1 is positively correlated with the stage and grade of lung tumors and related to a poor prognosis for the patients with early-stage lung cancer. ALDH1 is therefore a lung tumor stem cell-associated marker. These findings offer an important new tool for the study of lung CSCs and provide a potential prognostic factor and therapeutic target for treatment of the patients with lung cancer. (Mol Cancer Res 2009;7(3):330–8)read more
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Tobacco nitrosamine NNK increases ALDH-positive cells via ROS-Wnt signaling pathway in A549 human lung cancer cells.
TL;DR: The effects of NNK on the CSCs in A549 human lung cancer cells using flow cytometry with aldehyde dehydrogenase (ALDH), a functional marker of C SCs, are investigated and it is found that NNK increased the proportion of ALDH-positive cells in a dose-dependent manner.
Journal ArticleDOI
microRNA-520a-3p inhibits proliferation and cancer stem cell phenotype by targeting HOXD8 in non-small cell lung cancer
TL;DR: It is concluded that microRNA-520a-3p inhibits proliferation and cancer stem cell phenotype by targeting HOXD8 in NSCLC cells and restoration of microRNAs might be a therapeutic strategy to reverse gefitinib resistance.
Journal ArticleDOI
Glyoxalase 1 gene is highly expressed in basal-like human breast cancers and contributes to survival of ALDH1-positive breast cancer stem cells.
Shoma Tamori,Yuka Nozaki,Hitomi Motomura,Hiromi Nakane,Reika Katayama,Chotaro Onaga,Eriko Kikuchi,Nami Shimada,Yuhei Suzuki,Mei Noike,Yasushi Hara,Keiko Sato,Tsugumichi Sato,Kouji Yamamoto,Kouji Yamamoto,Takehisa Hanawa,Misa Imai,Ryo Abe,Ryo Abe,Atsushi Yoshimori,Ryoko Takasawa,Sei-ichi Tanuma,Kazunori Akimoto +22 more
TL;DR: It is concluded that GLO1 is a potential therapeutic target for treatment of basal-like breast cancers and essential for the survival of ALDH1-positive breast cancer stem cells.
Journal ArticleDOI
Cancer stem cells are enriched in Fanconi anemia head and neck squamous cell carcinomas
TL;DR: FA-HNSCC are highly enriched for CSC and may serve as a model to develop CSC-targeted therapies for HNSCC, and enhanced in vitro tumorsphere formation compared to sporadic H NSCC cells.
Journal ArticleDOI
Mining distinct aldehyde dehydrogenase 1 (ALDH1) isoenzymes in gastric cancer
TL;DR: It was found that when compared with normal tissues, ALDH1A1 mRNA expression was downregulated, whereas AL DH1A3 andALDH1B1 were upregulated in GC patients, and high transcription activity of ALDH 1A1 predicted better OS in gastric intestinal type adenocarcinoma, but not in diffuse gastric adenOCarcinomas.
References
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Prospective identification of tumorigenic breast cancer cells
Muhammad Al-Hajj,Max S. Wicha,Adalberto Benito-Hernandez,Sean J. Morrison,Sean J. Morrison,Michael F. Clarke +5 more
TL;DR: The ability to prospectively identify tumorigenic cancer cells will facilitate the elucidation of pathways that regulate their growth and survival and strategies designed to target this population may lead to more effective therapies.
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Stem cells, cancer, and cancer stem cells
TL;DR: Stem cell biology has come of age: Unequivocal proof that stem cells exist in the haematopoietic system has given way to the prospective isolation of several tissue-specific stem and progenitor cells, the initial delineation of their properties and expressed genetic programmes, and the beginnings of their utility in regenerative medicine.
Journal ArticleDOI
ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome.
Christophe Ginestier,Min Hee Hur,Emmanuelle Charafe-Jauffret,Florence Monville,Julie Dutcher,Marty Brown,Jocelyne Jacquemier,Patrice Viens,Celina G. Kleer,Suling Liu,Anne F. Schott,Daniel F. Hayes,Daniel Birnbaum,Max S. Wicha,Gabriela Dontu +14 more
TL;DR: It is shown that normal and cancer human mammary epithelial cells with increased aldehyde dehydrogenase activity (ALDH) have stem/progenitor properties and these cells contain the subpopulation of normal breast epithelium with the broadest lineage differentiation potential and greatest growth capacity in a xenotransplant model.
Journal ArticleDOI
Tumour stem cells and drug resistance
TL;DR: Gaining a better insight into the mechanisms of stem-cell resistance to chemotherapy might lead to new therapeutic targets and better anticancer strategies.
Journal ArticleDOI
Identification of Bronchioalveolar Stem Cells in Normal Lung and Lung Cancer
Carla F. Kim,Erica L. Jackson,Amber Woolfenden,Sharon Lawrence,Imran Babar,Sinae Vogel,Denise Crowley,Roderick T. Bronson,Tyler Jacks +8 more
TL;DR: Although bronchiolar cells and alveolar cells are proposed to be the precursor cells of adenocarcinoma, this work points to bronchioalveolar stem cells as the putative cells of origin for this subtype of lung cancer.