Aldehyde dehydrogenase 1 is a tumor stem cell-associated marker in lung cancer.
Feng Jiang,Qi Qiu,Abha Khanna,Nevins W. Todd,Janaki Deepak,Lingxiao Xing,Huijun Wang,Zhenqiu Liu,Yun Su,Sanford A. Stass,Ruth L. Katz +10 more
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TLDR
Immunohistochemical analysis of 303 clinical specimens from three independent cohorts of lung cancer patients and controls show that expression of ALDH1 is positively correlated with the stage and grade of lung tumors and related to a poor prognosis for the patients with early-stage lung cancer.Abstract:
Tumor contains small population of cancer stem cells (CSC) that are responsible for its maintenance and relapse. Analysis of these CSCs may lead to effective prognostic and therapeutic strategies for the treatment of cancer patients. We report here the identification of CSCs from human lung cancer cells using Aldefluor assay followed by fluorescence-activated cell sorting analysis. Isolated cancer cells with relatively high aldehyde dehydrogenase 1 (ALDH1) activity display in vitro features of CSCs, including capacities for proliferation, self-renewal, and differentiation, resistance to chemotherapy, and expressing CSC surface marker CD133. In vivo experiments show that the ALDH1-positive cells could generate tumors that recapitulate the heterogeneity of the parental cancer cells. Immunohistochemical analysis of 303 clinical specimens from three independent cohorts of lung cancer patients and controls show that expression of ALDH1 is positively correlated with the stage and grade of lung tumors and related to a poor prognosis for the patients with early-stage lung cancer. ALDH1 is therefore a lung tumor stem cell-associated marker. These findings offer an important new tool for the study of lung CSCs and provide a potential prognostic factor and therapeutic target for treatment of the patients with lung cancer. (Mol Cancer Res 2009;7(3):330–8)read more
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Identification of brain tumour initiating cells using the stem cell marker aldehyde dehydrogenase
Seung Ah Choi,Seung Ah Choi,Ji Yeoun Lee,Ji Yeoun Lee,Ji Hoon Phi,Ji Hoon Phi,Kyu-Chang Wang,Kyu-Chang Wang,Chul-Kee Park,Sung Hye Park,Seung-Ki Kim,Seung-Ki Kim +11 more
TL;DR: The data suggest that primary brain tumours contain distinct subpopulations of cells that have high expression levels of ALDH and BTIC characteristics, and ALDH might be a potential therapeutic target applicable to primarybrain tumours.
Journal ArticleDOI
Normal Hematopoietic Stem Cells within the AML Bone Marrow Have a Distinct and Higher ALDH Activity Level than Co-Existing Leukemic Stem Cells
Gerrit Jan Schuurhuis,Michaël H. Meel,Floris Wouters,Lisa A. Min,Monique Terwijn,Nick A. de Jonge,Angèle Kelder,Alexander N Snel,Sonja Zweegman,Gert J. Ossenkoppele,Linda Smit +10 more
TL;DR: It is shown that normal CD34+CD38– HSC present in AML bone marrow are a distinct sub-population of cells with high ALDH activity (ALDHbright), which form the basis for combined functionally and immunophenotypically based identification and purification of LSC and HSC within the AML marrow, aiming at development of highly specific anti-LSC therapy.
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Aberrant Expression of Retinoic Acid Signaling Molecules Influences Patient Survival in Astrocytic Gliomas
Benito Campos,Franz-Simon Centner,Justo Lorenzo Bermejo,Justo Lorenzo Bermejo,Ramadan Ali,Katharina Dorsch,Feng Wan,Feng Wan,Jörg Felsberg,Rezvan Ahmadi,Niels Grabe,Guido Reifenberger,Andreas Unterberg,Jürgen Burhenne,Christel Herold-Mende +14 more
TL;DR: The data indicate a complex and clinically relevant deregulation of RA signaling, which seems to be a central event in glioma pathogenesis, influencing patient prognosis.
Journal ArticleDOI
Sulforaphane inhibits cancer stem-like cell properties and cisplatin resistance through miR-214-mediated downregulation of c-MYC in non-small cell lung cancer
Qian-Qian Li,You-Ke Xie,Yue Wu,Linlin Li,Ying Liu,Xiao-Bo Miao,Qiuzhen Liu,Kaitai Yao,Guang-Hui Xiao +8 more
TL;DR: Sulforaphane, a potent anti-cancer and well-tolerated dietary compound, inhibits cancer stem-like cell (CSC) properties and enhances therapeutic efficacy of cisplatin in human non-small cell lung cancer (NSCLC).
Journal ArticleDOI
Aldehyde dehydrogenase is used by cancer cells for energy metabolism.
Joon Hee Kang,Seon-Hyeong Lee,Dongwan Hong,Jae Seon Lee,Hee-Sung Ahn,Ju-Hyun Ahn,Tae Wha Seong,Chang Hun Lee,Hyonchol Jang,Kyeong Man Hong,Cheolju Lee,Jae-Ho Lee,Soo-Youl Kim +12 more
TL;DR: This study revealed that NADH production by ALDH contributes significantly to ATP production in NSCLC, and gossypol, a pan-ALDH inhibitor, markedly reduced the level of ATP.
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ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome.
Christophe Ginestier,Min Hee Hur,Emmanuelle Charafe-Jauffret,Florence Monville,Julie Dutcher,Marty Brown,Jocelyne Jacquemier,Patrice Viens,Celina G. Kleer,Suling Liu,Anne F. Schott,Daniel F. Hayes,Daniel Birnbaum,Max S. Wicha,Gabriela Dontu +14 more
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Journal ArticleDOI
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Carla F. Kim,Erica L. Jackson,Amber Woolfenden,Sharon Lawrence,Imran Babar,Sinae Vogel,Denise Crowley,Roderick T. Bronson,Tyler Jacks +8 more
TL;DR: Although bronchiolar cells and alveolar cells are proposed to be the precursor cells of adenocarcinoma, this work points to bronchioalveolar stem cells as the putative cells of origin for this subtype of lung cancer.