Aldehyde dehydrogenase 1 is a tumor stem cell-associated marker in lung cancer.
Feng Jiang,Qi Qiu,Abha Khanna,Nevins W. Todd,Janaki Deepak,Lingxiao Xing,Huijun Wang,Zhenqiu Liu,Yun Su,Sanford A. Stass,Ruth L. Katz +10 more
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TLDR
Immunohistochemical analysis of 303 clinical specimens from three independent cohorts of lung cancer patients and controls show that expression of ALDH1 is positively correlated with the stage and grade of lung tumors and related to a poor prognosis for the patients with early-stage lung cancer.Abstract:
Tumor contains small population of cancer stem cells (CSC) that are responsible for its maintenance and relapse. Analysis of these CSCs may lead to effective prognostic and therapeutic strategies for the treatment of cancer patients. We report here the identification of CSCs from human lung cancer cells using Aldefluor assay followed by fluorescence-activated cell sorting analysis. Isolated cancer cells with relatively high aldehyde dehydrogenase 1 (ALDH1) activity display in vitro features of CSCs, including capacities for proliferation, self-renewal, and differentiation, resistance to chemotherapy, and expressing CSC surface marker CD133. In vivo experiments show that the ALDH1-positive cells could generate tumors that recapitulate the heterogeneity of the parental cancer cells. Immunohistochemical analysis of 303 clinical specimens from three independent cohorts of lung cancer patients and controls show that expression of ALDH1 is positively correlated with the stage and grade of lung tumors and related to a poor prognosis for the patients with early-stage lung cancer. ALDH1 is therefore a lung tumor stem cell-associated marker. These findings offer an important new tool for the study of lung CSCs and provide a potential prognostic factor and therapeutic target for treatment of the patients with lung cancer. (Mol Cancer Res 2009;7(3):330–8)read more
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Expression and Functional Role of ALDH1 in Cervical Carcinoma Cells
Qun-Xian Rao,Tingting Yao,Bingzhong Zhang,Rongchun Lin,Zhi-Liao Chen,Hui Zhou,Lijuan Wang,Huaiwu Lu,Qin Chen,Na Di,Zhongqiu Lin +10 more
TL;DR: Evaluated ALDH1 expression in CaSki, HeLa and SiHa cervical cancer cells using the Aldefluor method suggested that AL DH1 is a marker of cervical cancer stem cells and expand the understanding of its functional role.
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Targeting cancer stem cells in solid tumors by vitamin D.
Jae Young So,Nanjoo Suh +1 more
TL;DR: Recent findings about the roles of Notch, Hedgehog, Wnt, and TGF-β signaling in CSCs as well as the effects of vitamin D on these stem cell signaling pathways are summarized.
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Prognostic significance of stem cell-related marker expression and its correlation with histologic subtypes in lung adenocarcinoma
Eunhyang Park,Soo Young Park,Ping Li Sun,Ping Li Sun,Yan Jin,Yan Jin,Ji-Eun Kim,Sanghoon Jheon,Kwhanmien Kim,Choon Taek Lee,Hyojin Kim,Jin Haeng Chung +11 more
TL;DR: Results suggest that Nanog is an independent negative prognostic factor that may be associated with epithelial-mesenchymal transition in lung adenocarcinoma.
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Nicotine increases cancer stem cell population in MCF-7 cells.
TL;DR: Investigation of the effects of nicotine on the population of cancer stem cells in MCF-7 human breast cancer cells, using flow cytometry with a cancer stem cell marker aldehyde dehydrogenase, finds that nicotine increased ALDH-positive cell population in a dose-dependent manner and demonstrates that a PKC-Notch pathway is involved.
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The Stemness Phenotype Model
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Journal ArticleDOI
ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome.
Christophe Ginestier,Min Hee Hur,Emmanuelle Charafe-Jauffret,Florence Monville,Julie Dutcher,Marty Brown,Jocelyne Jacquemier,Patrice Viens,Celina G. Kleer,Suling Liu,Anne F. Schott,Daniel F. Hayes,Daniel Birnbaum,Max S. Wicha,Gabriela Dontu +14 more
TL;DR: It is shown that normal and cancer human mammary epithelial cells with increased aldehyde dehydrogenase activity (ALDH) have stem/progenitor properties and these cells contain the subpopulation of normal breast epithelium with the broadest lineage differentiation potential and greatest growth capacity in a xenotransplant model.
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Tumour stem cells and drug resistance
TL;DR: Gaining a better insight into the mechanisms of stem-cell resistance to chemotherapy might lead to new therapeutic targets and better anticancer strategies.
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Identification of Bronchioalveolar Stem Cells in Normal Lung and Lung Cancer
Carla F. Kim,Erica L. Jackson,Amber Woolfenden,Sharon Lawrence,Imran Babar,Sinae Vogel,Denise Crowley,Roderick T. Bronson,Tyler Jacks +8 more
TL;DR: Although bronchiolar cells and alveolar cells are proposed to be the precursor cells of adenocarcinoma, this work points to bronchioalveolar stem cells as the putative cells of origin for this subtype of lung cancer.