Aldehyde dehydrogenase 1 is a tumor stem cell-associated marker in lung cancer.
Feng Jiang,Qi Qiu,Abha Khanna,Nevins W. Todd,Janaki Deepak,Lingxiao Xing,Huijun Wang,Zhenqiu Liu,Yun Su,Sanford A. Stass,Ruth L. Katz +10 more
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TLDR
Immunohistochemical analysis of 303 clinical specimens from three independent cohorts of lung cancer patients and controls show that expression of ALDH1 is positively correlated with the stage and grade of lung tumors and related to a poor prognosis for the patients with early-stage lung cancer.Abstract:
Tumor contains small population of cancer stem cells (CSC) that are responsible for its maintenance and relapse. Analysis of these CSCs may lead to effective prognostic and therapeutic strategies for the treatment of cancer patients. We report here the identification of CSCs from human lung cancer cells using Aldefluor assay followed by fluorescence-activated cell sorting analysis. Isolated cancer cells with relatively high aldehyde dehydrogenase 1 (ALDH1) activity display in vitro features of CSCs, including capacities for proliferation, self-renewal, and differentiation, resistance to chemotherapy, and expressing CSC surface marker CD133. In vivo experiments show that the ALDH1-positive cells could generate tumors that recapitulate the heterogeneity of the parental cancer cells. Immunohistochemical analysis of 303 clinical specimens from three independent cohorts of lung cancer patients and controls show that expression of ALDH1 is positively correlated with the stage and grade of lung tumors and related to a poor prognosis for the patients with early-stage lung cancer. ALDH1 is therefore a lung tumor stem cell-associated marker. These findings offer an important new tool for the study of lung CSCs and provide a potential prognostic factor and therapeutic target for treatment of the patients with lung cancer. (Mol Cancer Res 2009;7(3):330–8)read more
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Lung cancer stem cell: Fancy conceptual model of tumor biology or cornerstone of a forthcoming therapeutic breakthrough?
Tony Sourisseau,Khaled A. Hassan,Ignacio I. Wistuba,Frédérique Penault-Llorca,Julien Adam,Eric Deutsch,Jean-Charles Soria +6 more
TL;DR: In this paper, the authors discuss the data suggesting the existence of cancer stem cells in lung tumors and the potential clinical utility of CSCs as prognostic markers or cellular targets of new therapeutic strategies.
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Proline-rich tyrosine kinase 2 and its phosphorylated form pY881 are novel prognostic markers for non-small-cell lung cancer progression and patients' overall survival.
TL;DR: Both Pyk2 and phosphorylated PyK2[pY881] are potential prognostic factors and therapeutic targets for NSCLC.
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High metabolic rate and stem cell characteristics of esophageal cancer stem-like cells depend on the Hsp27-AKT-HK2 pathway.
Chen Chi Liu,Chen Chi Liu,Kun Ta Chou,Kun Ta Chou,Jyuan Wei Hsu,Jyuan Wei Hsu,Jiun Han Lin,Jiun Han Lin,Tien Wei Hsu,Tien Wei Hsu,David Hung-Tsang Yen,David Hung-Tsang Yen,Shih-Chieh Hung,Shih-Chieh Hung,Han Shui Hsu,Han Shui Hsu +15 more
TL;DR: It is demonstrated that ESCC cell lines in spheroid culture display CSC phenotypes, including increased ALDH activity, chemoresistance and tumor initiation, which are dependent on Hsp27 activation and HK2 activation, and stem cells features and aberrant metabolic reprogramming of esophageal CSCs depend on the Hsp 27–AKT–HK2 pathway.
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Aggressive tumor microenvironment of solid predominant lung adenocarcinoma subtype harboring with epidermal growth factor receptor mutations.
Koichi Saruwatari,Shinnosuke Ikemura,Keigo Sekihara,Takeshi Kuwata,Satoshi Fujii,Shigeki Umemura,Keisuke Kirita,Shingo Matsumoto,Kiyotaka Yoh,Seiji Niho,Hironobu Ohmatsu,Atsushi Ochiai,Hirotsugu Kohrogi,Masahiro Tsuboi,Koichi Goto,Genichiro Ishii +15 more
TL;DR: Compared with other predominant subtypes with EGFR mutations, the microenvironment of SPA with EG FR mutations is characterized by cancer cells with higher invasive and immune evasion potential and more abundant stromal cells with tumor-promoting functions, which would contribute to the more aggressive behavior of S PA.
Journal ArticleDOI
Isolation and Identification of Cancer Stem-Like Cells in Adenocarcinoma and Squamous Cell Carcinoma of the Lung: A Pilot Study.
Valentina Masciale,Giulia Grisendi,Federico Banchelli,Roberto D'Amico,Antonino Maiorana,Pamela Sighinolfi,Alessandro Stefani,Uliano Morandi,Massimo Dominici,Beatrice Aramini +9 more
TL;DR: In vitro functional assays demonstrated that ALDH high cells exhibited migration capacity with distinct behaviors between ALDHhigh spheres in ADENO vs. SQUAMO samples, which may suggest new differential approaches for prognostic and therapeutic purposes in patients with non-small-cell lung cancer.
References
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ALDH1 is a marker of normal and malignant human mammary stem cells and a predictor of poor clinical outcome.
Christophe Ginestier,Min Hee Hur,Emmanuelle Charafe-Jauffret,Florence Monville,Julie Dutcher,Marty Brown,Jocelyne Jacquemier,Patrice Viens,Celina G. Kleer,Suling Liu,Anne F. Schott,Daniel F. Hayes,Daniel Birnbaum,Max S. Wicha,Gabriela Dontu +14 more
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Identification of Bronchioalveolar Stem Cells in Normal Lung and Lung Cancer
Carla F. Kim,Erica L. Jackson,Amber Woolfenden,Sharon Lawrence,Imran Babar,Sinae Vogel,Denise Crowley,Roderick T. Bronson,Tyler Jacks +8 more
TL;DR: Although bronchiolar cells and alveolar cells are proposed to be the precursor cells of adenocarcinoma, this work points to bronchioalveolar stem cells as the putative cells of origin for this subtype of lung cancer.