Aldehyde dehydrogenase 1 is a tumor stem cell-associated marker in lung cancer.
Feng Jiang,Qi Qiu,Abha Khanna,Nevins W. Todd,Janaki Deepak,Lingxiao Xing,Huijun Wang,Zhenqiu Liu,Yun Su,Sanford A. Stass,Ruth L. Katz +10 more
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TLDR
Immunohistochemical analysis of 303 clinical specimens from three independent cohorts of lung cancer patients and controls show that expression of ALDH1 is positively correlated with the stage and grade of lung tumors and related to a poor prognosis for the patients with early-stage lung cancer.Abstract:
Tumor contains small population of cancer stem cells (CSC) that are responsible for its maintenance and relapse. Analysis of these CSCs may lead to effective prognostic and therapeutic strategies for the treatment of cancer patients. We report here the identification of CSCs from human lung cancer cells using Aldefluor assay followed by fluorescence-activated cell sorting analysis. Isolated cancer cells with relatively high aldehyde dehydrogenase 1 (ALDH1) activity display in vitro features of CSCs, including capacities for proliferation, self-renewal, and differentiation, resistance to chemotherapy, and expressing CSC surface marker CD133. In vivo experiments show that the ALDH1-positive cells could generate tumors that recapitulate the heterogeneity of the parental cancer cells. Immunohistochemical analysis of 303 clinical specimens from three independent cohorts of lung cancer patients and controls show that expression of ALDH1 is positively correlated with the stage and grade of lung tumors and related to a poor prognosis for the patients with early-stage lung cancer. ALDH1 is therefore a lung tumor stem cell-associated marker. These findings offer an important new tool for the study of lung CSCs and provide a potential prognostic factor and therapeutic target for treatment of the patients with lung cancer. (Mol Cancer Res 2009;7(3):330–8)read more
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JNK Signaling Mediates EPHA2-Dependent Tumor Cell Proliferation, Motility, and Cancer Stem Cell–like Properties in Non–Small Cell Lung Cancer
TL;DR: It is shown that the JNK/c-JUN signaling mediates EPHA2-dependent tumor cell proliferation and motility and a role forEPHA2 in CSC function is found, providing evidence for EPHA 2 as a potential therapeutic target in NSCLC.
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The cardiac atrial appendage stem cell: a new and promising candidate for myocardial repair
Remco Koninckx,Annick Daniëls,Severina Windmolders,Urbain Mees,Regina Macianskiene,Kanigula Mubagwa,Paul Steels,Luc Jamaer,Jasperina Dubois,Boris Robic,Marc Hendrikx,Jean-Luc Rummens,Karen Hensen +12 more
TL;DR: It is suggested that CASCs are an intrinsic stem cell population and are not mobilized from bone marrow or peripheral blood, compared with cardiosphere-derived cells, and possess an enhanced cardiac differentiation capacity.
Journal ArticleDOI
ALDH/CD44 identifies uniquely tumorigenic cancer stem cells in salivary gland mucoepidermoid carcinomas
April Adams,Kristy A. Warner,Alexander T. Pearson,Zhaocheng Zhang,Hong Sun Kim,Daiki Mochizuki,Gregory J. Basura,Joseph I. Helman,Andrea Mantesso,Andrea Mantesso,Rogerio M. Castilho,Max S. Wicha,Jacques E. Nör +12 more
TL;DR: Investigation of the expression of putative cancer stem cell markers in primary human mucoepidermoid carcinomas by immunofluorescence, in vitro salisphere assays, and in vivo tumorigenicity assays in immunodeficient mice demonstrate that salivary gland mucoEPidermoids contain a small population of cancer stem cells with enhanced tumorigenic potential and that are characterized by high ALDH activity and CD44 expression.
Journal ArticleDOI
Differential expression of aldehyde dehydrogenase 1a1 (ALDH1) in normal ovary and serous ovarian tumors
TL;DR: AlDH1 expression in the ovary does not appear to be similar to breast, lung or colon cancer suggesting possible functional differences in these cancers and provides a basis for further study of the mechanism of ALDH1 in this process.
Journal ArticleDOI
An Embryonic Stem Cell–Like Signature Identifies Poorly Differentiated Lung Adenocarcinoma but not Squamous Cell Carcinoma
TL;DR: It is suggested that not all poorly differentiated non–small cell lung cancers exhibit a gene expression profile similar to that of ESC, and that other characteristics may play a more important role in the determination of differentiation and survival in SCC of the lung.
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Carla F. Kim,Erica L. Jackson,Amber Woolfenden,Sharon Lawrence,Imran Babar,Sinae Vogel,Denise Crowley,Roderick T. Bronson,Tyler Jacks +8 more
TL;DR: Although bronchiolar cells and alveolar cells are proposed to be the precursor cells of adenocarcinoma, this work points to bronchioalveolar stem cells as the putative cells of origin for this subtype of lung cancer.