Broad host range of SARS-CoV-2 and the molecular basis for SARS-CoV-2 binding to cat ACE2
Lili Wu,Qian Chen,Qian Chen,Kefang Liu,Kefang Liu,Jia Wang,Pengcheng Han,Yanfang Zhang,Yu Hu,Yu Hu,Yumin Meng,Xiaoqian Pan,Chengpeng Qiao,Siyu Tian,Pei Du,Hao Song,Weifeng Shi,Jianxun Qi,Hong-Wei Wang,Jinghua Yan,George F. Gao,Qihui Wang,Qihui Wang +22 more
TLDR
Cutting light on pursuing the intermediate host of SARS-CoV-2 is shed and the necessity of monitoring susceptible hosts to prevent further outbreaks is highlighted.Abstract:
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the recent pandemic COVID-19, is reported to have originated from bats, with its intermediate host unknown to date. Here, we screened 26 animal counterparts of the human ACE2 (hACE2), the receptor for SARS-CoV-2 and SARS-CoV, and found that the ACE2s from various species, including pets, domestic animals and multiple wild animals, could bind to SARS-CoV-2 receptor binding domain (RBD) and facilitate the transduction of SARS-CoV-2 pseudovirus. Comparing to SARS-CoV-2, SARS-CoV seems to have a slightly wider range in choosing its receptor. We further resolved the cryo-electron microscopy (cryo-EM) structure of the cat ACE2 (cACE2) in complex with the SARS-CoV-2 RBD at a resolution of 3 A, revealing similar binding mode as hACE2 to the SARS-CoV-2 RBD. These results shed light on pursuing the intermediate host of SARS-CoV-2 and highlight the necessity of monitoring susceptible hosts to prevent further outbreaks.read more
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Receptor binding and complex structures of human ACE2 to spike RBD from omicron and delta SARS-CoV-2
Pengcheng Han,Lin-jie Li,Sheng Liu,Qisheng Wang,Di Zhang,Zepeng Xu,Pu Han,Xiaomei Li,Qi Peng,Chao Su,Baihan Huang,Dedong Li,Rong Zhang,M. Tian,Lutang Fu,Yuanzhu Gao,Xin Zhao,Kefang Liu,Jianxun Qi,George F. Gao,Peiyi Wang +20 more
TL;DR: In this article , the binding properties between the human receptor ACE2 (hACE2) and the VOC RBDs were studied and the crystal and cryoelectron microscopy structures of the omicron RBD-hACE 2 complex were resolved.
Journal ArticleDOI
Identification of novel bat coronaviruses sheds light on the evolutionary origins of SARS-CoV-2 and related viruses.
Hong Zhou,Jingkai Ji,Xing Chen,Yuhai Bi,Juan Li,Qihui Wang,Tao Hu,Hao Song,Runchu Zhao,Yanhua Chen,Mingxue Cui,Yanyan Zhang,Alice C. Hughes,Alice C. Hughes,Edward C. Holmes,Weifeng Shi +15 more
TL;DR: A meta-transcriptomic study of 411 bat samples collected from a small geographical region in Yunnan province, China, between May 2019 and November 2020 as mentioned in this paper identified 24 full-length coronavirus genomes.
Journal ArticleDOI
Cell entry by SARS-CoV-2.
TL;DR: In this paper, the authors discuss the recent advances in understanding the molecular events during SARS-CoV-2 entry which will contribute to developing vaccines and therapeutics, and discuss some auxiliary receptors and cofactors are also involved that expand the host/tissue tropism.
Journal ArticleDOI
Molecular insights into receptor binding of recent emerging SARS-CoV-2 variants.
Pengcheng Han,Pengcheng Han,Pengcheng Han,Chao Su,Chao Su,Yanfang Zhang,Chongzhi Bai,Anqi Zheng,Chengpeng Qiao,Qing Wang,Sheng Niu,Sheng Niu,Qian Chen,Qian Chen,Yuqin Zhang,Weiwei Li,Hanyi Liao,Jing Li,Zengyuan Zhang,Heecheol Cho,Mengsu Yang,Xiaoyu Rong,Xiaoyu Rong,Yu Hu,Yu Hu,Niu Huang,Jinghua Yan,Qihui Wang,Xin Zhao,George F. Gao,George F. Gao,Jianxun Qi +31 more
TL;DR: In this paper, the authors examined several SARS-CoV-2 variants of concern (VOCs) including Alpha, Beta, and Gamma, and demonstrated that five variants receptor-binding domain (RBD) increased binding affinity for hACE2, and four variants pseudoviruses increased entry into susceptible cells.
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