Chemical reporters for biological discovery
Markus Grammel,Howard C. Hang +1 more
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TLDR
How chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms is reviewed.Abstract:
Functional tools are needed to understand complex biological systems. Here we review how chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms. By tagging these biomolecules, researchers can now monitor their dynamics in living systems and discover specific substrates of cellular pathways. These advances in chemical biology are thus providing important tools to characterize biological pathways and are poised to facilitate our understanding of human diseases.read more
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Click Chemistry in Complex Mixtures: Bioorthogonal Bioconjugation
Craig S. McKay,M. G. Finn +1 more
TL;DR: An update on recent developments in bioorthogonal chemistry that highlights key advances in reaction rates, biocompatibility, and applications is provided.
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Finding the Right (Bioorthogonal) Chemistry
TL;DR: The most common classes of bioorthogonal chemistries are compared and compared and a framework for matching the reactions with downstream applications is provided to refine the understanding of living systems.
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Designing logical codon reassignment – Expanding the chemistry in biology
TL;DR: In this paper, a focused review of methods for UAA incorporation with an emphasis on the different tRNA synthetase/tRNA pairs exploited or developed, focusing upon the different UAA structures that have been incorporated and the logic behind the design and future creation of such systems.
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Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering
TL;DR: In this paper, the authors used stimulated Raman-scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals.
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Bioorthogonal chemical imaging of metabolic activities in live mammalian hippocampal tissues with stimulated Raman scattering
TL;DR: Bioorthogonal chemical imaging of DNA, RNA, protein and lipid metabolism in live rat brain hippocampal tissues is demonstrated by coupling stimulated Raman scattering microscopy with integrated deuterium and alkyne labeling and paves the way for the study of complex metabolic profiles in live brain tissue under both physiological and pathological conditions with single-cell resolution and minimal perturbation.
References
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Hening Lin,Xiaoyang Su,Bin He +2 more
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Chemical reporters for fluorescent detection and identification of O-GlcNAc-modified proteins reveal glycosylation of the ubiquitin ligase NEDD4-1
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Jessica E. Rexach,Peter M. Clark,Daniel E. Mason,Rachael L. Neve,Eric C. Peters,Linda C. Hsieh-Wilson +5 more
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