Chemical reporters for biological discovery
Markus Grammel,Howard C. Hang +1 more
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TLDR
How chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms is reviewed.Abstract:
Functional tools are needed to understand complex biological systems. Here we review how chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms. By tagging these biomolecules, researchers can now monitor their dynamics in living systems and discover specific substrates of cellular pathways. These advances in chemical biology are thus providing important tools to characterize biological pathways and are poised to facilitate our understanding of human diseases.read more
Citations
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Changes in Metabolic Chemical Reporter Structure Yield a Selective Probe of O-GlcNAc Modification
TL;DR: It is demonstrated here that 6-azido-6-deoxy-N-acetyl-glucosamine (6AzGlcNAc) is a specific MCR for O- GlcNAcylated proteins, and results suggest that specific M CRs for other types of glycosylation can be developed.
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1,2,4-Triazines Are Versatile Bioorthogonal Reagents.
David N Kamber,Yong Liang,Robert J. Blizzard,Fang Liu,Ryan A. Mehl,K. N. Houk,Jennifer A. Prescher +6 more
TL;DR: A new class of bioorthogonal reagents, 1,2,4-triazines, capable of robust reactivity with trans-cyclooctene (TCO) and enhanced stability of the triazine scaffold enabled its direct use in recombinant protein production.
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Orthogonal bioorthogonal chemistries.
TL;DR: The development of orthogonal bioorthogonal reactions and their application in multi-target imaging and macromolecule assembly are highlighted, along with prospects for identifying new classes of compatible reactions.
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Transition-metal-mediated uncaging in living human cells—an emerging alternative to photolabile protecting groups.
TL;DR: Metal catalysts based on palladium, ruthenium and iron are discussed and their biocompatibility and catalytic efficiency in uncaging reactions within biological environments are discussed.
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Metabolic Remodeling of Cell-Surface Sialic Acids: Principles, Applications, and Recent Advances
TL;DR: The metabolic glycan labeling (MGL) strategy has emerged as an invaluable chemical biology tool that enables metabolic installation of useful functionalities into cell‐surface sialoglycans by “hijacking” the sialic acid biosynthetic pathway.
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Yu-Fei He,Bin-Zhong Li,Zheng Li,Peng Liu,Yang Wang,Qingyu Tang,Jianping Ding,Yingying Jia,Zhangcheng Chen,Lin Li,Yan Sun,Xiuxue Li,Qing Dai,Chun-Xiao Song,Kangling Zhang,Chuan He,Guoliang Xu +16 more
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