Chemical reporters for biological discovery
Markus Grammel,Howard C. Hang +1 more
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TLDR
How chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms is reviewed.Abstract:
Functional tools are needed to understand complex biological systems. Here we review how chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms. By tagging these biomolecules, researchers can now monitor their dynamics in living systems and discover specific substrates of cellular pathways. These advances in chemical biology are thus providing important tools to characterize biological pathways and are poised to facilitate our understanding of human diseases.read more
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Exploring interactions between extracellular vesicles and cells for innovative drug delivery system design.
TL;DR: Extracellular vesicles (EVs) are submicron cell-secreted structures containing proteins, nucleic acids and lipids as mentioned in this paper, which can functionally transfer these cargoes from one cell to another to modulate physiological and pathological processes.
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Aqueous Oxidative Heck Reaction as a Protein-Labeling Strategy
Maria E. Ourailidou,Jan-Ytzen van der Meer,Bert-Jan Baas,Margot Jeronimus-Stratingh,Aditya L. Gottumukkala,Gerrit J. Poelarends,Adriaan J. Minnaard,Frank J. Dekker +7 more
TL;DR: The palladium‐catalyzed oxidative Heck reaction is presented as a new and robust bio‐orthogonal strategy for linking functionalized arylboronic acids to protein‐bound alkenes in high yields and with excellent chemoselectivity even in the presence of complex protein mixtures from living cells.
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Synthesis, characterization and biological evaluation of labile intercalative ruthenium(ii) complexes for anticancer drug screening.
TL;DR: Ru2-Ru4 exhibited stronger DNA transcription inhibition activity, higher cell uptake efficiency and better anticancer activity than Ru1, and was the most toxic complex toward all cancer cells which inhibited DNA replication and transcription.
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Fortschritt in Richtung bioorthogonaler Katalyse mit Organometallverbindungen
TL;DR: In this article, a Methode zur intrazellularen, katalytischen Aktivierung einer Zytostatikavorstufe, welche die Apoptose effizient einleitete.
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BLISS: A Bioorthogonal Dual-Labeling Strategy to Unravel Lignification Dynamics in Plants.
Cédric Lion,Clémence Simon,Brigitte Huss,Anne-Sophie Blervacq,Louis Tirot,Djadidi Toybou,Corentin Spriet,Christian Slomianny,Yann Guérardel,Simon Hawkins,Christophe Biot +10 more
TL;DR: An original bioorthogonal labeling imaging sequential strategy (BLISS) is developed to visualize and analyze the incorporation of both p-hydroxyphenyl (H) and guaiacyl (G) units into lignin in vivo with a combination of strain-promoted and copper-catalyzed azide-alkyne cycloadditions.
References
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