Chemical reporters for biological discovery
Markus Grammel,Howard C. Hang +1 more
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TLDR
How chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms is reviewed.Abstract:
Functional tools are needed to understand complex biological systems. Here we review how chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms. By tagging these biomolecules, researchers can now monitor their dynamics in living systems and discover specific substrates of cellular pathways. These advances in chemical biology are thus providing important tools to characterize biological pathways and are poised to facilitate our understanding of human diseases.read more
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Kinetic resolution of cyclic benzylic azides enabled by site- and enantioselective C(sp3)–H oxidation
TL;DR: In this paper , an effective nonenzymatic kinetic resolution (KR) of cyclic benzylic azides through site-and enantioselective C(sp3)-H oxidation is described.
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Chemoproteomic Analysis of Microbiota Metabolite-Protein Targets and Mechanisms.
TL;DR: The continued development of specific microbiota metabolite reporters and more precise proteomic methods should reveal new microbiota mechanisms of action, therapeutic targets, and biomarkers for a variety of human diseases.
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Nuclear Receptor Chemical Reporter Enables Domain-Specific Analysis of Ligands in Mammalian Cells.
TL;DR: A domain-specific covalent chemical reporter is developed for peroxisome proliferator-activated receptors (PPARs) and its utility to screen and characterize the potency of candidate NR ligands in live cells is demonstrated.
Study of organic probes and strategies for DNA fluorescent labelling: from basic photochemistry to super-resolution optical microscopy
TL;DR: This chapter concerns to an investigation of an optimal sequence-specific DNA fluorescent labelling, using super-resolution fluorescence microscopy, and explores a biological system at the singlemolecule level.
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The Promise and Challenge of Digital Biology.
Mark Minie,Ram Samudrala +1 more
TL;DR: The era of Digital Biology began in 2010 with the “rebooting” of a bacterial cell using a synthetic DNA genome created from a digital template stored on a computer, and with this event, came the first complete proof that DNA was the true software of life.
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Shinsuke Ito,Li Shen,Qing Dai,Susan C. Wu,Leonard B. Collins,James A. Swenberg,Chuan He,Yi Zhang +7 more
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