Chemical reporters for biological discovery
Markus Grammel,Howard C. Hang +1 more
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TLDR
How chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms is reviewed.Abstract:
Functional tools are needed to understand complex biological systems. Here we review how chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms. By tagging these biomolecules, researchers can now monitor their dynamics in living systems and discover specific substrates of cellular pathways. These advances in chemical biology are thus providing important tools to characterize biological pathways and are poised to facilitate our understanding of human diseases.read more
Citations
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Journal ArticleDOI
A localized molecular automaton for in situ visualization of proteins with specific chemical modifications
TL;DR: A localized DNA automaton is reported for in situ visualization of a specific protein subtype with dual chemical modifications on the cell surface, which executes protein-confined computation according to an anticoding–coding propagation algorithm.
Journal ArticleDOI
A Chemical Genetic Method for Monitoring Genome-Wide Dynamics of O-GlcNAc Turnover on Chromatin-Associated Proteins
TL;DR: DNA precipitation methods are used to enable monitoring time-dependent turnover of O-GlcNAc modified proteins associated with chromatin and detail methods for coded blinding of samples alongside new normalization strategies to enable time-resolved, genome-wide analyses using chemical genetic methods.
Book ChapterDOI
Identifying potentially O-GlcNAcylated proteins using metabolic labeling, bioorthogonal enrichment, and Western blotting.
TL;DR: This work describes how to combine one of these approaches, metabolic chemical reporters (MCRs), with bioorthogonal chemistry and Western blotting to identify potentially O-GlcNAcylated proteins.
Journal ArticleDOI
Design and development of histone deacetylase (HDAC) chemical probes for cell-based profiling
Victoria E. Albrow,Rachel L. Grimley,James A. Clulow,Colin R. Rose,Jianmin Sun,Joseph S. Warmus,Edward W. Tate,Lyn H. Jones,R. Ian Storer +8 more
TL;DR: Small molecule probes with class IIa and pan-HDAC activity that contain photoreactive crosslinking groups and either a biotin reporter, or a terminal alkyne handle for subsequent bioorthogonal ligation are reported.
Journal ArticleDOI
A versatile AIE fluorogen with selective reactivity to primary amines for monitoring amination, protein labeling, and mitochondrial staining
Xinyuan He,Huilin Xie,Lianrui Hu,Peng-Chao Liu,Changhuo Xu,Wei He,Wutong Du,Siwei Zhang,Hao Xing,Xinyue Liu,Hojeong Park,Tsz Shing Cheung,Min-Hui Li,Ryan T. K. Kwok,Jacky Wing Yip Lam,Jian Lu,Ben Zhong Tang +16 more
TL;DR: A chromone-based aggregation-induced emission (AIE) fluorogen called CMVMN is reported as a self-reporting bioconjugation reagent for selective primary amine identification, and its applications for monitoring bioprocesses of amination and protein labeling are reported.
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