Chemical reporters for biological discovery
Markus Grammel,Howard C. Hang +1 more
Reads0
Chats0
TLDR
How chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms is reviewed.Abstract:
Functional tools are needed to understand complex biological systems. Here we review how chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms. By tagging these biomolecules, researchers can now monitor their dynamics in living systems and discover specific substrates of cellular pathways. These advances in chemical biology are thus providing important tools to characterize biological pathways and are poised to facilitate our understanding of human diseases.read more
Citations
More filters
Journal ArticleDOI
Click Chemistry in Complex Mixtures: Bioorthogonal Bioconjugation
Craig S. McKay,M. G. Finn +1 more
TL;DR: An update on recent developments in bioorthogonal chemistry that highlights key advances in reaction rates, biocompatibility, and applications is provided.
Journal ArticleDOI
Finding the Right (Bioorthogonal) Chemistry
TL;DR: The most common classes of bioorthogonal chemistries are compared and compared and a framework for matching the reactions with downstream applications is provided to refine the understanding of living systems.
Journal ArticleDOI
Designing logical codon reassignment – Expanding the chemistry in biology
TL;DR: In this paper, a focused review of methods for UAA incorporation with an emphasis on the different tRNA synthetase/tRNA pairs exploited or developed, focusing upon the different UAA structures that have been incorporated and the logic behind the design and future creation of such systems.
Journal ArticleDOI
Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering
TL;DR: In this paper, the authors used stimulated Raman-scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals.
Journal ArticleDOI
Bioorthogonal chemical imaging of metabolic activities in live mammalian hippocampal tissues with stimulated Raman scattering
TL;DR: Bioorthogonal chemical imaging of DNA, RNA, protein and lipid metabolism in live rat brain hippocampal tissues is demonstrated by coupling stimulated Raman scattering microscopy with integrated deuterium and alkyne labeling and paves the way for the study of complex metabolic profiles in live brain tissue under both physiological and pathological conditions with single-cell resolution and minimal perturbation.
References
More filters
Journal ArticleDOI
Click-mediated labeling of bacterial membranes through metabolic modification of the lipopolysaccharide inner core.
TL;DR: This work investigated whether another sugar could be used as a target for the metabolic modification of glycans, and found 3-deoxy-d-mannooctulosonic acid (KDO) appears to be a very attractive candidate.
Journal ArticleDOI
Chemical approaches to understanding O-GlcNAc glycosylation in the brain
TL;DR: Some of the emerging roles for O-GlcNAc in the nervous system are highlighted and how chemical tools have significantly advanced understanding of the scope, functional significance and cellular dynamics of this modification are described.
Journal ArticleDOI
Cell-selective metabolic glycan labeling based on ligand-targeted liposomes.
TL;DR: A cell-specific metabolic glycan labeling strategy has been developed using azidosugars encapsulated in ligand-targeted liposomes designed to bind specific cell-surface receptors that are only expressed or up-regulated in target cells.
Journal ArticleDOI
Enzymatic Site‐Specific Functionalization of Protein Methyltransferase Substrates with Alkynes for Click Labeling
Wibke Peters,Sophie Willnow,Mike Duisken,Henning Kleine,Thomas Macherey,Kelly E. Duncan,David W. Litchfield,Bernhard Lüscher,Elmar Weinhold +8 more
TL;DR: The main protein MTase substrates described are core histones and a few proteins associated with gene transcription, and synthetic double-activated AdoMet analogues with allylic and propargylic methyl group replacements for site-specific DNA modification by DNA MTases.
Journal ArticleDOI
Lipid-independent Secretion of a Drosophila Wnt Protein
TL;DR: It is found that WntD, a recently characterized Drosophila Wnt family member, does not require Porcupine or Wntless/Evi/Sprinter for its secretion or signaling activity, and it is suggested that different modes of secretion may exist for different Wnt proteins.