Chemical reporters for biological discovery
Markus Grammel,Howard C. Hang +1 more
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TLDR
How chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms is reviewed.Abstract:
Functional tools are needed to understand complex biological systems. Here we review how chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms. By tagging these biomolecules, researchers can now monitor their dynamics in living systems and discover specific substrates of cellular pathways. These advances in chemical biology are thus providing important tools to characterize biological pathways and are poised to facilitate our understanding of human diseases.read more
Citations
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Posted ContentDOI
Click-ExM enables expansion microscopy for all biomolecules
TL;DR: click-ExM as discussed by the authors integrates click-labeling into ExM to enable a "one-stop-shop" method for nanoscale imaging of various types of biomolecules.
Journal ArticleDOI
Chemistry-enabled methods for the visualization of cell-surface glycoproteins in Metazoans.
Kelly N. Chuh,Matthew R. Pratt +1 more
TL;DR: The chemical methods that have been created to enable the visualization of the major types of cell-surface glycoproteins in living systems are reviewed, from mammalian cells to whole animals.
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Synthesis of novel l-rhamnose derived acyclic C-nucleosides with substituted 1,2,3-triazole core as potent sodium-glucose co-transporter (SGLT) inhibitors.
Siddamal Reddy Putapatri,Abhinav Kanwal,Sanjay K. Banerjee,Sanjay K. Banerjee,Srinivas Kantevari,Srinivas Kantevari +5 more
TL;DR: The utility of acyclic C-nucleosides as novel SGLT inhibitors for future investigations is demonstrated, with compound 7a-o with IC50: 125.9nM emerged as the most potent S GLT2 inhibitor and compound 7d exhibited best selectivity for inhibition of SGLt2.
Journal ArticleDOI
Live-cell imaging and profiling of c-Jun N-terminal kinases using covalent inhibitor-derived probes
TL;DR: Small-molecule JNK-targeting probes are reported based on a covalent inhibitor to show that some probes may be suitable for live-cell imaging and profiling of JNKs.
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Rapid Screening and Identification of Living Pathogenic Organisms via Optimized Bioorthogonal Non-canonical Amino Acid Tagging.
Allison R. Sherratt,Allison R. Sherratt,Yanouchka Rouleau,Yanouchka Rouleau,Christian C. Luebbert,Miroslava Strmiskova,Teodor Veres,Sabah Bidawid,Nathalie Corneau,John Paul Pezacki,John Paul Pezacki +10 more
TL;DR: An optimized bioorthogonal non-canonical amino acid tagging approach to the imaging, capture, and interrogation of shigatoxigenic/verotoxigenics Escherichia coli and Listeria that enables the distinction between living wild-type pathogenic bacteria.
References
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Tet proteins can convert 5-methylcytosine to 5-formylcytosine and 5-carboxylcytosine
Shinsuke Ito,Li Shen,Qing Dai,Susan C. Wu,Leonard B. Collins,James A. Swenberg,Chuan He,Yi Zhang +7 more
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Tet-Mediated Formation of 5-Carboxylcytosine and Its Excision by TDG in Mammalian DNA
Yu-Fei He,Bin-Zhong Li,Zheng Li,Peng Liu,Yang Wang,Qingyu Tang,Jianping Ding,Yingying Jia,Zhangcheng Chen,Lin Li,Yan Sun,Xiuxue Li,Qing Dai,Chun-Xiao Song,Kangling Zhang,Chuan He,Guoliang Xu +16 more
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