Chemical reporters for biological discovery
Markus Grammel,Howard C. Hang +1 more
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TLDR
How chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms is reviewed.Abstract:
Functional tools are needed to understand complex biological systems. Here we review how chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms. By tagging these biomolecules, researchers can now monitor their dynamics in living systems and discover specific substrates of cellular pathways. These advances in chemical biology are thus providing important tools to characterize biological pathways and are poised to facilitate our understanding of human diseases.read more
Citations
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Global profiling of protein lipidation using chemical proteomic technologies.
Edward W. Tate,Karunakaran A. Kalesh,Thomas Lanyon-Hogg,Elisabeth M. Storck,Emmanuelle Thinon +4 more
TL;DR: Key modifications include acylation, prenylations, cholesterylation and GPI anchors, which are an essential modification (PTM) in all forms of life.
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Bioorthogonal Chemical Reporters for Selective In Situ Probing of Mycomembrane Components in Mycobacteria
TL;DR: Mycobacteria-specific chemical reporters that can selectively probe either covalent arabinogalactan mycolates or non-covalent trehalosemycolates in live mycob bacteria are described and enable selective in-situ detection of the major MM components.
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Developing bioorthogonal probes to span a spectrum of reactivities
TL;DR: This Review highlights recent advances in the development of bioorthogonal reactions, focusing on how principles of physical organic chemistry have guided probe design and how mechanistic insights have driven the field.
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Discovery of new mutually orthogonal bioorthogonal cycloaddition pairs through computational screening
TL;DR: The sydnone-dibenzocyclooctyne and norbornene-tetrazine cycloadditions are both bioorthogonal and mutually orthogonal, used for simultaneous labeling of two targets.
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Global Analysis of Protein N-Myristoylation and Exploration of N-Myristoyltransferase as a Drug Target in the Neglected Human Pathogen Leishmania donovani
Megan H. Wright,Daniel Paape,Elisabeth M. Storck,Remigiusz A. Serwa,Deborah F. Smith,Edward W. Tate +5 more
TL;DR: This work constitutes the first global experimental analysis of protein lipidation in Leishmania, and reveals the extent of NMT-related biology yet to be explored for this neglected human pathogen.
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