Chemical reporters for biological discovery
Markus Grammel,Howard C. Hang +1 more
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TLDR
How chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms is reviewed.Abstract:
Functional tools are needed to understand complex biological systems. Here we review how chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms. By tagging these biomolecules, researchers can now monitor their dynamics in living systems and discover specific substrates of cellular pathways. These advances in chemical biology are thus providing important tools to characterize biological pathways and are poised to facilitate our understanding of human diseases.read more
Citations
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Illuminating biological processes through site-specific protein labeling
TL;DR: This tutorial review introduces various strategies for site-specific protein labeling that utilize artificial peptide sequences or genetically encoded UAAs as the labeling handle and introduces the recently emerged dual site- specific protein labeling strategies.
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Illumination of growth, division and secretion by metabolic labeling of the bacterial cell surface
TL;DR: This review describes aspects of bacterial growth, division and secretion that have recently been uncovered by metabolic labeling of the cell envelope and discusses the potential of these techniques for translational applications.
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Global, in situ, site-specific analysis of protein S-sulfenylation
TL;DR: This protocol describes global, in situ, site-specific analysis of protein S-sulfenylation using sulfenic acid–specific chemical probes and mass spectrometry (MS)-based proteomics and outlines strategies for quantitative analysis of this modification in cells responding to redox perturbations.
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Designed heterogeneous palladium catalysts for reversible light-controlled bioorthogonal catalysis in living cells
TL;DR: A versatile light-controlled bioorthogonal catalyst built by modifying macroporous silica-Pd0 with supramolecular complex of azobenzene and β-cyclodextrin that can be regulated by light-induced structural changes, mimicking allosteric regulation mechanism of bio-enzymes.
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Optimized Tetrazine Derivatives for Rapid Bioorthogonal Decaging in Living Cells
Xinyuan Fan,Xinyuan Fan,Yun Ge,Feng Lin,Yang Yang,Gong Zhang,Gong Zhang,William Shu Ching Ngai,Zhi Lin,Siqi Zheng,Jie Wang,Jie Wang,Jingyi Zhao,Jingyi Zhao,Jie Li,Peng Chen,Peng Chen +16 more
TL;DR: 3,6-substituted TZ derivatives showed remarkably enhanced decaging rates, enabling rapid iDA-mediated protein activation in living cells and promoted us to design and test unsymmetric TZs bearing an EWG group and a small non-EWG group at the 3- and 6-position.
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