Chemical reporters for biological discovery
Markus Grammel,Howard C. Hang +1 more
Reads0
Chats0
TLDR
How chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms is reviewed.Abstract:
Functional tools are needed to understand complex biological systems. Here we review how chemical reporters in conjunction with bioorthogonal labeling methods can be used to image and retrieve nucleic acids, proteins, glycans, lipids and other metabolites in vitro, in cells as well as in whole organisms. By tagging these biomolecules, researchers can now monitor their dynamics in living systems and discover specific substrates of cellular pathways. These advances in chemical biology are thus providing important tools to characterize biological pathways and are poised to facilitate our understanding of human diseases.read more
Citations
More filters
Journal ArticleDOI
Click Chemistry in Complex Mixtures: Bioorthogonal Bioconjugation
Craig S. McKay,M. G. Finn +1 more
TL;DR: An update on recent developments in bioorthogonal chemistry that highlights key advances in reaction rates, biocompatibility, and applications is provided.
Journal ArticleDOI
Finding the Right (Bioorthogonal) Chemistry
TL;DR: The most common classes of bioorthogonal chemistries are compared and compared and a framework for matching the reactions with downstream applications is provided to refine the understanding of living systems.
Journal ArticleDOI
Designing logical codon reassignment – Expanding the chemistry in biology
TL;DR: In this paper, a focused review of methods for UAA incorporation with an emphasis on the different tRNA synthetase/tRNA pairs exploited or developed, focusing upon the different UAA structures that have been incorporated and the logic behind the design and future creation of such systems.
Journal ArticleDOI
Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering
TL;DR: In this paper, the authors used stimulated Raman-scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals.
Journal ArticleDOI
Bioorthogonal chemical imaging of metabolic activities in live mammalian hippocampal tissues with stimulated Raman scattering
TL;DR: Bioorthogonal chemical imaging of DNA, RNA, protein and lipid metabolism in live rat brain hippocampal tissues is demonstrated by coupling stimulated Raman scattering microscopy with integrated deuterium and alkyne labeling and paves the way for the study of complex metabolic profiles in live brain tissue under both physiological and pathological conditions with single-cell resolution and minimal perturbation.
References
More filters
Journal ArticleDOI
Metabolic click-labeling with a fucose analog reveals pectin delivery, architecture, and dynamics in Arabidopsis cell walls.
TL;DR: Details of polysaccharide delivery, organization, and dynamics in cell walls are revealed, including that the pectin network is reoriented in elongating root epidermal cells.
Journal ArticleDOI
DHHC5 Protein Palmitoylates Flotillin-2 and Is Rapidly Degraded on Induction of Neuronal Differentiation in Cultured Cells
TL;DR: It is found that down-regulation of DHHC5 was triggered within minutes following growth factor withdrawal from normal neural stem cells, a maneuver that is used to induce neural differentiation in culture and suggest that protein palmitoylation can be regulated through changes in DHHC PAT levels in response to differentiation signals.
Journal ArticleDOI
The cellular and molecular basis of bitter tastant-induced bronchodilation.
TL;DR: In this article, G-protein-coupled bitter taste receptors (TAS2Rs) were found to activate largeconductance Ca2+-activated K+ channels, which in turn hyperpolarize the membrane, leading to relaxation.
Journal ArticleDOI
Clickable NAD Analogues for Labeling Substrate Proteins of Poly(ADP-ribose) Polymerases
TL;DR: It is demonstrated that tubulin and three mitochondrial proteins, TRAP1 (TNF receptor-associated protein 1), citrate synthase, and GDH (glutamate dehydrogenase 1), are substrates of PARP-1 in vitro.
Journal ArticleDOI
Labeling substrates of protein arginine methyltransferase with engineered enzymes and matched S-adenosyl-L-methionine analogues.
TL;DR: The development of a clickable SAM analogue cofactor, 4-propargyloxy-but-2-enyl SAM, and its implementation to label substrates of human protein arginine methyltransferase 1 (PRMT1) are reported, showing potential to label and identify PMT targets in the context of a complex cellular mixture.