Chromatin motion is constrained by association with nuclear compartments in human cells.
Reads0
Chats0
TLDR
It is shown that loci at nucleoli or the nuclear periphery are significantly less mobile than other, more nucleoplasmic loci, suggesting a role for the nucleolus and nuclear periphery in maintaining the three-dimensional organization of chromatin in the human nucleus.About:
This article is published in Current Biology.The article was published on 2002-03-19 and is currently open access. It has received 570 citations till now. The article focuses on the topics: Scaffold/matrix attachment region & Chromatin.read more
Citations
More filters
Journal ArticleDOI
Single-cell Hi-C reveals cell-to-cell variability in chromosome structure
Takashi Nagano,Yaniv Lubling,Tim J. Stevens,Stefan Schoenfelder,Eitan Yaffe,Wendy Dean,Ernest D. Laue,Amos Tanay,Peter Fraser +8 more
TL;DR: Single-cell Hi-C data bridge current gaps between genomics and microscopy studies of chromosomes, demonstrating how modular organization underlies dynamic chromosome structure, and how this structure is probabilistically linked with genome activity patterns.
Journal ArticleDOI
Pathways of chaperone-mediated protein folding in the cytosol
TL;DR: In the cytosol of prokaryotic and eukaryotic cells, molecular chaperones of different structural classes form a network of pathways that can handle substrate polypeptides from the point of initial synthesis on ribosomes to the final stages of folding.
Journal ArticleDOI
Active genes dynamically colocalize to shared sites of ongoing transcription.
Cameron S. Osborne,Lyubomira Chakalova,Karen E. Brown,David R. F. Carter,David R. F. Carter,Alice Horton,Emmanuel Debrand,Beatriz Goyenechea,Jennifer A. Mitchell,Susana Lopes,Susana Lopes,Wolf Reik,Peter Fraser +12 more
TL;DR: It is shown that, during transcription in vivo, distal genes colocalize to the same transcription factory at high frequencies.
Journal ArticleDOI
Exploring the three-dimensional organization of genomes: interpreting chromatin interaction data
TL;DR: Several types of statistical and computational approaches that have recently been developed to analyse chromatin interaction data are described.
Journal ArticleDOI
Mediator and RNA polymerase II clusters associate in transcription-dependent condensates
Won-Ki Cho,Jan-Hendrik Spille,Micca Hecht,Choongman Lee,Charles H. Li,Valentin Grube,Valentin Grube,Ibrahim I Cisse +7 more
TL;DR: This work used live-cell superresolution and light-sheet imaging to study the organization and dynamics of the Mediator coactivator and RNA polymerase II (Pol II) directly and suggests that large clusters of Mediator, recruited by transcription factors at large or clustered enhancer elements, interact with large Pol II clusters in transcriptional condensates in vivo.
References
More filters
Journal ArticleDOI
Genomic sequencing
George M. Church,Walter Gilbert +1 more
TL;DR: The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.
Book
Random walks in biology
TL;DR: This book is a lucid, straightforward introduction to the concepts and techniques of statistical physics that students of biology, biochemistry, and biophysics must know.
Journal ArticleDOI
Chromosome territories, nuclear architecture and gene regulation in mammalian cells.
TL;DR: The emerging view is that chromosomes are compartmentalized into discrete territories and the location of a gene within a chromosome territory seems to influence its access to the machinery responsible for specific nuclear functions, such as transcription and splicing.
Nuclear architecture and gene regulation in mammalian cells
TL;DR: The emerging view is that chromosomes are compartmentalized into discrete territories, and the location of a gene within a chromosome territory seems to influence its access to the machinery responsible for specific nuclear functions, such as transcription and splicing.
Journal ArticleDOI
High mobility of proteins in the mammalian cell nucleus
Robert D. Phair,Tom Misteli +1 more
TL;DR: It is shown that many nuclear proteins roam the cell nucleus in vivo and that nuclear compartments are the reflection of the steady-state association/dissociation of its ‘residents’ with the nucleoplasmic space.
Related Papers (5)
Chromosome territories, nuclear architecture and gene regulation in mammalian cells.
Comprehensive mapping of long-range interactions reveals folding principles of the human genome.
Erez Lieberman Aiden,Nynke L. van Berkum,Louise Williams,Maxim Imakaev,Tobias Ragoczy,Tobias Ragoczy,Agnes Telling,Agnes Telling,Ido Amit,Bryan R. Lajoie,Peter J. Sabo,Michael O. Dorschner,Richard Sandstrom,Bradley E. Bernstein,Bradley E. Bernstein,Michaël Bender,Mark Groudine,Mark Groudine,Andreas Gnirke,John A. Stamatoyannopoulos,Leonid A. Mirny,Eric S. Lander,Eric S. Lander,Job Dekker +23 more