Journal ArticleDOI
Circulating miR-192 in liver fluke-associated cholangiocarcinoma patients: a prospective prognostic indicator.
Runglawan Silakit,Watcharin Loilome,Puangrat Yongvanit,P. Chusorn,Anchalee Techasen,Thidarut Boonmars,Narong Khuntikeo,Nittaya Chamadol,Chawalit Pairojkul,Nisana Namwat +9 more
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TLDR
This study aimed to investigate the miR‐192 levels in patients' sera of liver fluke‐associated cholangiocarcinoma (CCA) for a prospective prognostic indicator.Abstract:
Background
This study aimed to investigate the miR-192 levels in patients' sera of liver fluke-associated cholangiocarcinoma (CCA) for a prospective prognostic indicator.
Methods
MicroRNA polymerase chain reaction (PCR) array was performed using pooled serum samples from 11 CCA patients and nine healthy subjects. Selected miRNAs were verified for the differential levels in both sera and tumor tissues (of patients and Opisthorchis viverrini (Ov)-induced CCA model) using TaqMan miRNA expression assay.
Results
Our results demonstrated that miR-192 was significantly higher in the serum of CCA patients than that in healthy subjects giving a sensitivity of 74% and specificity of 72% (area under the curve [AUC] = 0.803; 95% confidence interval [CI], 0.708–0.897, P < 0.0001). Serum miR-192 examined in Ov infected subjects and subjects with periductal fibrosis were increased but not statistically significantly when compared with healthy subjects. High levels of serum miR-192 were significantly correlated with lymph node metastasis (P = 0.047) and shorter survival compared with individuals with low levels of serum miR-192 (hazard ratio [HR] 2.076, 95% CI 1.004–4.291, P = 0.049). We also found that the expression levels of miR-192 appeared to be elevated in both CCA tissues of patients and in Ov-induced CCA tissues of a hamster model.
Conclusions
This finding indicates that elevated levels of miR-192 may be involved in CCA genesis and have a potential utility as a noninvasive prognostic indicator for CCA patients.read more
Citations
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Journal ArticleDOI
Cholangiocarcinoma 2020: the next horizon in mechanisms and management
Jesus M. Banales,Jesus M. Banales,Jesus M. Banales,Jose J.G. Marin,Jose J.G. Marin,Angela Lamarca,Angela Lamarca,Pedro M. Rodrigues,Shahid A. Khan,Lewis R. Roberts,Vincenzo Cardinale,Guido Carpino,Jesper B. Andersen,Chiara Braconi,Diego F. Calvisi,Maria J. Perugorria,Maria J. Perugorria,Luca Fabris,Luca Fabris,Luke Boulter,Rocio I.R. Macias,Rocio I.R. Macias,Eugenio Gaudio,Domenico Alvaro,Sergio A. Gradilone,Mario Strazzabosco,Mario Strazzabosco,Marco Marzioni,Cédric Coulouarn,Laura Fouassier,Chiara Raggi,Pietro Invernizzi,Joachim C. Mertens,Anja Moncsek,Sumera Rizvi,Julie K. Heimbach,Bas Groot Koerkamp,Jordi Bruix,Jordi Bruix,Alejandro Forner,Alejandro Forner,John Bridgewater,Juan W. Valle,Juan W. Valle,Gregory J. Gores +44 more
TL;DR: This expert Consensus Statement, endorsed by the ENS-CCA, summarizes the latest advances in CCA, including classification, genetics and treatment, and provides recommendations for CCA management and priorities across basic, translational and clinical research.
Journal ArticleDOI
MiR-21 promotes intrahepatic cholangiocarcinoma proliferation and growth in vitro and in vivo by targeting PTPN14 and PTEN.
Lijuan Wang,Chenchen He,Xin Sui,Mengjiao Cai,Congya Zhou,Jinlu Ma,Lei Wu,Hao Wang,Suxia Han,Qing Zhu +9 more
TL;DR: This study revealed functional and mechanistic links between miR-21 and tumor suppressor genes, PTPN14 and PTEN, in the pathogenesis of ICC and found that its expression was significantly upregulated in serum of ICC patients.
Journal ArticleDOI
Diagnostic and prognostic biomarkers in cholangiocarcinoma.
Rocio I.R. Macias,Rocio I.R. Macias,Miroslaw Kornek,Pedro M. Rodrigues,Nuno A. Paiva,Rui E. Castro,Sabine K. Urban,Stephen P. Pereira,Massimiliano Cadamuro,Christian Rupp,Sven H. Loosen,Tom Luedde,Jesus M. Banales,Jesus M. Banales,Jesus M. Banales +14 more
TL;DR: This review summarizes and discusses the advances in the investigation of novel diagnostic and prognostic biomarkers in CCA and envisions the future directions in this field of research.
Journal ArticleDOI
The search for novel diagnostic and prognostic biomarkers in cholangiocarcinoma.
Rocio I.R. Macias,Rocio I.R. Macias,Jesus M. Banales,Bruno Sangro,Jordi Muntané,Matías A. Avila,Elisa Lozano,Maria J. Perugorria,Francisco J. Padillo,Luis Bujanda,Jose J.G. Marin +10 more
TL;DR: This review is an up-to-date of currently used biomarkers and the candidates with promising characteristics that could be included in the clinical practice in the next future.
Journal ArticleDOI
Intrahepatic cholangiocarcinoma: Molecular markers for diagnosis and prognosis
TL;DR: iCCA biomarkers are summarized and defined and the molecular mechanisms underlying iCCA carcinogenesis are defined to highlight potential diagnostic and prognostic application of molecular biomarkers.
References
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Journal ArticleDOI
MicroRNAs: Genomics, Biogenesis, Mechanism, and Function
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Circulating microRNAs as stable blood-based markers for cancer detection
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Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases
Xi Chen,Yi Ba,Lijia Ma,Lijia Ma,Xing Cai,Yuan Yin,Kehui Wang,Jigang Guo,Yujing Zhang,Jiangning Chen,Xing Guo,Qibin Li,Qibin Li,Xiaoying Li,Wenjing Wang,Yan Zhang,Jin Wang,Xueyuan Jiang,Yang Xiang,Chen Xu,Pingping Zheng,Juanbin Zhang,Ruiqiang Li,Hongjie Zhang,Xiaobin Shang,Ting Gong,Guang Ning,Jun Wang,Jun Wang,Ke Zen,Junfeng Zhang,Chen-Yu Zhang +31 more
TL;DR: It is demonstrated that miRNAs are present in the serum and plasma of humans and other animals such as mice, rats, bovine fetuses, calves, and horses, and can serve as potential biomarkers for the detection of various cancers and other diseases.
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TL;DR: In this paper, the effects of miRNA dysregulation in the cellular pathways that lead to the progressive conversion of normal cells into cancer cells and the potential to develop new molecular miRNA-targeted therapies are discussed.
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TL;DR: This review briefly describes miRNA biogenesis and discusses how miRNAs can act as oncogenes and tumor suppressors and the role of miRNAAs in the diagnosis, prognosis, and treatment of cancer.
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