Clinical Applications of DNA Vaccines: Current Progress
Bernadette Ferraro,Matthew P. Morrow,Natalie A. Hutnick,Thomas H. Shin,Colleen E. Lucke,David B. Weiner +5 more
TLDR
The ability of the current, or second-generation, DNA vaccines to induce more-potent cellular and humoral responses opens up this platform to be examined in both preventative and therapeutic arenas.Abstract:
It was discovered almost 20 years ago that plasmid DNA, when injected into the skin or muscle of mice, could induce immune responses to encoded antigens. Since that time, there has since been much progress in understanding the basic biology behind this deceptively simple vaccine platform and much technological advancement to enhance immune potency. Among these advancements are improved formulations and improved physical methods of delivery, which increase the uptake of vaccine plasmids by cells; optimization of vaccine vectors and encoded antigens; and the development of novel formulations and adjuvants to augment and direct the host immune response. The ability of the current, or second-generation, DNA vaccines to induce more-potent cellular and humoral responses opens up this platform to be examined in both preventative and therapeutic arenas. This review focuses on these advances and discusses both preventive and immunotherapeutic clinical applications.read more
Citations
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Journal ArticleDOI
Liquid–Liquid Equilibrium and Extraction Performance of Aqueous Biphasic Systems Composed of Water, Cholinium Carboxylate Ionic Liquids and K2CO3
TL;DR: In this article, a novel ABS composed of Ionic liquid-based aqueous biphasic systems (IL-based ABS) with a more acceptable environmental footprint and enhanced biocompatibility was investigated.
Journal ArticleDOI
Segregated growth kinetics of Escherichia coli DH5α-NH36 in exponential-fed perfusion culture for pDNA vaccine production.
Rodolfo Munguía-Soto,Aurora García-Rendón,Adriana Garibay-Escobar,Patricia Guerrero-Germán,Armando Tejeda-Mansir +4 more
TL;DR: A practical conclusion of this work is that a space–time yield analysis of a bioprocess requires a viability evaluation, and a new strategy of culture operation might help in the efficient production of pDNA for therapeutic use.
Dissertation
Roles in innate immune receptors: Host : pathogen interactions and strengthening vaccines
TL;DR: These studies will contribute to the understanding of the regulation of inflammasome activity, and how to deliver the next generation of DNA vaccines in combination with adjuvants.
Journal ArticleDOI
Vaccination against Bacterial Infections: Challenges, Progress, and New Approaches with a Focus on Intracellular Bacteria
TL;DR: This review provides an overview of existing antibacterial vaccines and novel approaches to vaccination with a focus on immunization against intracellular bacteria.
Book ChapterDOI
Lactic Acid Bacteria as Delivery Vehicle for Therapeutics Applications.
Viviane Lima Batista,Tales Fernando da Silva,Luís Cláudio Lima de Jesus,Ana Paula Tapia-Costa,Mariana Martins Drumond,Mariana Martins Drumond,Vasco Azevedo,Pamela Mancha-Agresti +7 more
TL;DR: DNA vaccines consist of the administration of a bacterial plasmid under the control of a eukaryotic promoter encoding the antigen of interest, capable of stimulating the immune system, becoming a promising technique for immunization against a variety of tumors and infection diseases.
References
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Journal ArticleDOI
Vaccination with ALVAC and AIDSVAX to Prevent HIV-1 Infection in Thailand
Supachai Rerks-Ngarm,Punnee Pitisuttithum,Sorachai Nitayaphan,Jaranit Kaewkungwal,Joseph Chiu,Robert Paris,Nakorn Premsri,Chawetsan Namwat,Mark de Souza,Elizabeth Adams,Michael Benenson,Sanjay Gurunathan,Jim Tartaglia,John G. McNeil,Donald P. Francis,Donald Stablein,Deborah L. Birx,Supamit Chunsuttiwat,Chirasak Khamboonruang,Prasert Thongcharoen,Merlin L. Robb,Nelson L. Michael,Prayura Kunasol,Jerome H. Kim +23 more
TL;DR: This ALVAC-HIV and AIDSVAX B/E vaccine regimen may reduce the risk of HIV infection in a community-based population with largely heterosexual risk and offer insight for future research.
Journal ArticleDOI
Heterologous protection against influenza by injection of DNA encoding a viral protein
Jeffrey B. Ulmer,John J. Donnelly,Suezanne E. Parker,Gary Rhodes,Philip L. Felgner,V. J. Dwarki,Stanislaw H. Gromkowski,R. Randall Deck,Corrille M. DeWitt,Arthur Friedman,Linda A. Hawe,Karen R. Leander,Douglas Martinez,Helen C. Perry,John W. Shiver,Donna L. Montgomery,Margaret A. Liu +16 more
TL;DR: To generate a viral antigen for presentation to the immune system without the limitations of direct peptide delivery or viral vectors, plasmid DNA encoding influenza A nucleop protein was injected into the quadriceps of BALB/c mice and resulted in the generation of nucleoprotein-specific CTLs.
Journal ArticleDOI
Efficacy assessment of a cell-mediated immunity HIV-1 vaccine (the Step Study): a double-blind, randomised, placebo-controlled, test-of-concept trial.
Susan Buchbinder,Devan V. Mehrotra,Ann Duerr,Daniel W. Fitzgerald,Robin Mogg,David Li,Peter B. Gilbert,Javier R. Lama,Michael Marmor,Carlos del Rio,M. Juliana McElrath,Danilo R. Casimiro,Keith Gottesdiener,Chodakewitz Jeffrey A,Lawrence Corey,Michael N. Robertson +15 more
TL;DR: This cell-mediated immunity vaccine did not prevent HIV-1 infection or reduce early viral level and Mechanisms for insufficient efficacy of the vaccine and the increased HIV- 1 infection rates in subgroups of vaccine recipients are being explored.
Journal ArticleDOI
Genetic immunization is a simple method for eliciting an immune response.
TL;DR: It is reported that an immune response can be elicited by introducing the gene encoding a protein directly into the skin of mice by using a hand-held form of the biolistic system.
Journal ArticleDOI
DNA vaccines: protective immunizations by parenteral, mucosal, and gene-gun inoculations
Ellen F. Fynan,Robert G. Webster,Deborah H. Fuller,Joel R. Haynes,Joseph C. Santoro,Harriet L. Robinson +5 more
TL;DR: By far the most efficient DNA immunizations were achieved by using a gene gun to deliver DNA-coated gold beads to the epidermis, and 95% protection was achieved by two immunizations with beads loaded with as little as 0.4 micrograms of DNA.