Clinical Applications of DNA Vaccines: Current Progress
Bernadette Ferraro,Matthew P. Morrow,Natalie A. Hutnick,Thomas H. Shin,Colleen E. Lucke,David B. Weiner +5 more
TLDR
The ability of the current, or second-generation, DNA vaccines to induce more-potent cellular and humoral responses opens up this platform to be examined in both preventative and therapeutic arenas.Abstract:
It was discovered almost 20 years ago that plasmid DNA, when injected into the skin or muscle of mice, could induce immune responses to encoded antigens. Since that time, there has since been much progress in understanding the basic biology behind this deceptively simple vaccine platform and much technological advancement to enhance immune potency. Among these advancements are improved formulations and improved physical methods of delivery, which increase the uptake of vaccine plasmids by cells; optimization of vaccine vectors and encoded antigens; and the development of novel formulations and adjuvants to augment and direct the host immune response. The ability of the current, or second-generation, DNA vaccines to induce more-potent cellular and humoral responses opens up this platform to be examined in both preventative and therapeutic arenas. This review focuses on these advances and discusses both preventive and immunotherapeutic clinical applications.read more
Citations
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Journal ArticleDOI
A novel DNA platform designed for vaccine use with high transgene expression and immunogenicity.
George Giorgi Babuadze,Jose Echanove,Claude Lamarre,Marc-Antoine deLaVega,Hugues Fausther-Bovendo,Trina Racine,Trina Racine,Alejandro M. Gomez,Hiva Azizi,Mathew Wade,Robert A. Kozak,Robert A. Kozak,Gary P. Kobinger +12 more
TL;DR: The pVax1 plasmid has been modified with a chicken β-actin promoter (CAG) and a post-transcriptional regulatory element, followed by a transcriptional termination signal as discussed by the authors.
Dissertation
Adjuvanted trimethyl chitosan based nanoparticle formulations to improve immunogenicity of DNA vaccines
TL;DR: TMC nanoparticles, formulated with Mtb antigen 85A expressing pDNA, successfully induced robust Th1 immune responses in mice and may render pDNA/Tmc nanoparticles a potential vaccine candidate for further investigations of protective efficacy against Mtb infections.
Journal ArticleDOI
Effect of Prophylactic Vaccination with the Membrane-Bound Acid Phosphatase Gene of Leishmania mexicana in the Murine Model of Localized Cutaneous Leishmaniasis.
María Angélica Burgos-Reyes,Lidia Baylón-Pacheco,Patricia Espíritu-Gordillo,Silvia Galindo-Gómez,Víctor Tsutsumi,José Luis Rosales-Encina +5 more
TL;DR: In this article, the LmxMBA gene of Leishmania mexicana was selected as a possible vaccine candidate using the reverse vaccinology approach, and the prophylactic effect generated by DNA vaccination with this gene in a murine model of cutaneous leishmaniasis was evaluated.
OtherDOI
Malaria vaccine development: over 40 years of trials and tribulations
TL;DR: Howard Engers was responsible for management of the leprosy and malaria vaccine research and development programs at the Tropical Disease Research program at the WHO in Geneva.
Journal ArticleDOI
A concise review of poultry vaccination and future implementation of plant-based vaccines
Naila Shahid,Abdul Qayyum Rao,P.E. Kristen,M. A. Ali,Bushra Tabassum,Sajid Umar,Sana Tahir,Ayesha Latif,Ammara Ahad,A A Shahid,Tayyab Husnain +10 more
TL;DR: In this article, the potential for plant-based vaccines and whether they are a good option to control poultry diseases is discussed and the challenges in the field of green vaccines are discussed.
References
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Journal ArticleDOI
Vaccination with ALVAC and AIDSVAX to Prevent HIV-1 Infection in Thailand
Supachai Rerks-Ngarm,Punnee Pitisuttithum,Sorachai Nitayaphan,Jaranit Kaewkungwal,Joseph Chiu,Robert Paris,Nakorn Premsri,Chawetsan Namwat,Mark de Souza,Elizabeth Adams,Michael Benenson,Sanjay Gurunathan,Jim Tartaglia,John G. McNeil,Donald P. Francis,Donald Stablein,Deborah L. Birx,Supamit Chunsuttiwat,Chirasak Khamboonruang,Prasert Thongcharoen,Merlin L. Robb,Nelson L. Michael,Prayura Kunasol,Jerome H. Kim +23 more
TL;DR: This ALVAC-HIV and AIDSVAX B/E vaccine regimen may reduce the risk of HIV infection in a community-based population with largely heterosexual risk and offer insight for future research.
Journal ArticleDOI
Heterologous protection against influenza by injection of DNA encoding a viral protein
Jeffrey B. Ulmer,John J. Donnelly,Suezanne E. Parker,Gary Rhodes,Philip L. Felgner,V. J. Dwarki,Stanislaw H. Gromkowski,R. Randall Deck,Corrille M. DeWitt,Arthur Friedman,Linda A. Hawe,Karen R. Leander,Douglas Martinez,Helen C. Perry,John W. Shiver,Donna L. Montgomery,Margaret A. Liu +16 more
TL;DR: To generate a viral antigen for presentation to the immune system without the limitations of direct peptide delivery or viral vectors, plasmid DNA encoding influenza A nucleop protein was injected into the quadriceps of BALB/c mice and resulted in the generation of nucleoprotein-specific CTLs.
Journal ArticleDOI
Efficacy assessment of a cell-mediated immunity HIV-1 vaccine (the Step Study): a double-blind, randomised, placebo-controlled, test-of-concept trial.
Susan Buchbinder,Devan V. Mehrotra,Ann Duerr,Daniel W. Fitzgerald,Robin Mogg,David Li,Peter B. Gilbert,Javier R. Lama,Michael Marmor,Carlos del Rio,M. Juliana McElrath,Danilo R. Casimiro,Keith Gottesdiener,Chodakewitz Jeffrey A,Lawrence Corey,Michael N. Robertson +15 more
TL;DR: This cell-mediated immunity vaccine did not prevent HIV-1 infection or reduce early viral level and Mechanisms for insufficient efficacy of the vaccine and the increased HIV- 1 infection rates in subgroups of vaccine recipients are being explored.
Journal ArticleDOI
Genetic immunization is a simple method for eliciting an immune response.
TL;DR: It is reported that an immune response can be elicited by introducing the gene encoding a protein directly into the skin of mice by using a hand-held form of the biolistic system.
Journal ArticleDOI
DNA vaccines: protective immunizations by parenteral, mucosal, and gene-gun inoculations
Ellen F. Fynan,Robert G. Webster,Deborah H. Fuller,Joel R. Haynes,Joseph C. Santoro,Harriet L. Robinson +5 more
TL;DR: By far the most efficient DNA immunizations were achieved by using a gene gun to deliver DNA-coated gold beads to the epidermis, and 95% protection was achieved by two immunizations with beads loaded with as little as 0.4 micrograms of DNA.