Clinical Applications of DNA Vaccines: Current Progress
Bernadette Ferraro,Matthew P. Morrow,Natalie A. Hutnick,Thomas H. Shin,Colleen E. Lucke,David B. Weiner +5 more
TLDR
The ability of the current, or second-generation, DNA vaccines to induce more-potent cellular and humoral responses opens up this platform to be examined in both preventative and therapeutic arenas.Abstract:
It was discovered almost 20 years ago that plasmid DNA, when injected into the skin or muscle of mice, could induce immune responses to encoded antigens. Since that time, there has since been much progress in understanding the basic biology behind this deceptively simple vaccine platform and much technological advancement to enhance immune potency. Among these advancements are improved formulations and improved physical methods of delivery, which increase the uptake of vaccine plasmids by cells; optimization of vaccine vectors and encoded antigens; and the development of novel formulations and adjuvants to augment and direct the host immune response. The ability of the current, or second-generation, DNA vaccines to induce more-potent cellular and humoral responses opens up this platform to be examined in both preventative and therapeutic arenas. This review focuses on these advances and discusses both preventive and immunotherapeutic clinical applications.read more
Citations
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Confronting the threat of SARS-CoV-2: Realities, challenges and therapeutic strategies (Review)
TL;DR: The molecular mechanisms that are responsible for SARS-CoV-2 infection are described and the progress of preclinical research into medical intervention against Sars- CoV- 2 infection are discussed.
Journal ArticleDOI
Optimizing in vivo gene transfer into mouse corpus cavernosum by use of surface electroporation.
Kang-Moon Song,Min Ji Choi,Mi-Hye Kwon,Kalyan Ghatak,Soo-Hwan Park,Dong-Soo Ryu,Ji-Kan Ryu,Jun-Kyu Suh +7 more
TL;DR: The optimal electroporation conditions for maximizing gene transfer into the corpus cavernosum of mice while avoiding damage to the erectile tissue are established and will be a valuable tool for gene therapy in the field of erectile dysfunction.
Journal ArticleDOI
An effective DNA priming-protein boosting approach for the cervical cancer vaccination
Zahra Kianmehr,Susan K. Ardestani,Hoorieh Soleimanjahi,Behrokh Farahmand,Asghar Abdoli,Maryam Khatami,Khadijeh Akbari,Fatemeh Fotouhi +7 more
TL;DR: It is suggested that prime-boost regimen can be considered as an efficient prophylactic and therapeutic vaccine.
Journal ArticleDOI
Tomorrow's vector vaccines for small ruminants.
TL;DR: New technology vaccines, namely DNA and recombinant viral vector vaccines, are being developed and tested against pathogens of small ruminants and examples of vaccines that have been tested against important diseases of sheep and goats are offered.
Journal ArticleDOI
Leishmania infantum pyridoxal kinase evaluated in a recombinant protein and DNA vaccine to protects against visceral leishmaniasis.
João A. Oliveira-da-Silva,Daniela P. Lage,Fernanda F. Ramos,Amanda S. Machado,Grasiele S.V. Tavares,Débora V.C. Mendonça,Isabela A.G. Pereira,Vívian T. Martins,Lívia M. Carvalho,Fernanda Ludolf,Thaís T.O. Santos,Thiago A.R. Reis,Camila Stefanie Fonseca de Oliveira,Raquel S. Bandeira,Alessandra M. Silva,Lourena E. Costa,Jamil S. Oliveira,Mariana C. Duarte,Daniel Menezes-Souza,Bruno Mendes Roatt,Antônio Lúcio Teixeira,Eduardo A.F. Coelho +21 more
TL;DR: Leishmania infantum pyridoxal kinase (PK) protein can be seen as a promising immunogen against human VL, protecting mice against L. infantum challenge when administered either as a DNA plasmid or recombinant protein plus adjuvant.
References
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Vaccination with ALVAC and AIDSVAX to Prevent HIV-1 Infection in Thailand
Supachai Rerks-Ngarm,Punnee Pitisuttithum,Sorachai Nitayaphan,Jaranit Kaewkungwal,Joseph Chiu,Robert Paris,Nakorn Premsri,Chawetsan Namwat,Mark de Souza,Elizabeth Adams,Michael Benenson,Sanjay Gurunathan,Jim Tartaglia,John G. McNeil,Donald P. Francis,Donald Stablein,Deborah L. Birx,Supamit Chunsuttiwat,Chirasak Khamboonruang,Prasert Thongcharoen,Merlin L. Robb,Nelson L. Michael,Prayura Kunasol,Jerome H. Kim +23 more
TL;DR: This ALVAC-HIV and AIDSVAX B/E vaccine regimen may reduce the risk of HIV infection in a community-based population with largely heterosexual risk and offer insight for future research.
Journal ArticleDOI
Heterologous protection against influenza by injection of DNA encoding a viral protein
Jeffrey B. Ulmer,John J. Donnelly,Suezanne E. Parker,Gary Rhodes,Philip L. Felgner,V. J. Dwarki,Stanislaw H. Gromkowski,R. Randall Deck,Corrille M. DeWitt,Arthur Friedman,Linda A. Hawe,Karen R. Leander,Douglas Martinez,Helen C. Perry,John W. Shiver,Donna L. Montgomery,Margaret A. Liu +16 more
TL;DR: To generate a viral antigen for presentation to the immune system without the limitations of direct peptide delivery or viral vectors, plasmid DNA encoding influenza A nucleop protein was injected into the quadriceps of BALB/c mice and resulted in the generation of nucleoprotein-specific CTLs.
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Efficacy assessment of a cell-mediated immunity HIV-1 vaccine (the Step Study): a double-blind, randomised, placebo-controlled, test-of-concept trial.
Susan Buchbinder,Devan V. Mehrotra,Ann Duerr,Daniel W. Fitzgerald,Robin Mogg,David Li,Peter B. Gilbert,Javier R. Lama,Michael Marmor,Carlos del Rio,M. Juliana McElrath,Danilo R. Casimiro,Keith Gottesdiener,Chodakewitz Jeffrey A,Lawrence Corey,Michael N. Robertson +15 more
TL;DR: This cell-mediated immunity vaccine did not prevent HIV-1 infection or reduce early viral level and Mechanisms for insufficient efficacy of the vaccine and the increased HIV- 1 infection rates in subgroups of vaccine recipients are being explored.
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Genetic immunization is a simple method for eliciting an immune response.
TL;DR: It is reported that an immune response can be elicited by introducing the gene encoding a protein directly into the skin of mice by using a hand-held form of the biolistic system.
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DNA vaccines: protective immunizations by parenteral, mucosal, and gene-gun inoculations
Ellen F. Fynan,Robert G. Webster,Deborah H. Fuller,Joel R. Haynes,Joseph C. Santoro,Harriet L. Robinson +5 more
TL;DR: By far the most efficient DNA immunizations were achieved by using a gene gun to deliver DNA-coated gold beads to the epidermis, and 95% protection was achieved by two immunizations with beads loaded with as little as 0.4 micrograms of DNA.