Clinical Applications of DNA Vaccines: Current Progress
Bernadette Ferraro,Matthew P. Morrow,Natalie A. Hutnick,Thomas H. Shin,Colleen E. Lucke,David B. Weiner +5 more
TLDR
The ability of the current, or second-generation, DNA vaccines to induce more-potent cellular and humoral responses opens up this platform to be examined in both preventative and therapeutic arenas.Abstract:
It was discovered almost 20 years ago that plasmid DNA, when injected into the skin or muscle of mice, could induce immune responses to encoded antigens. Since that time, there has since been much progress in understanding the basic biology behind this deceptively simple vaccine platform and much technological advancement to enhance immune potency. Among these advancements are improved formulations and improved physical methods of delivery, which increase the uptake of vaccine plasmids by cells; optimization of vaccine vectors and encoded antigens; and the development of novel formulations and adjuvants to augment and direct the host immune response. The ability of the current, or second-generation, DNA vaccines to induce more-potent cellular and humoral responses opens up this platform to be examined in both preventative and therapeutic arenas. This review focuses on these advances and discusses both preventive and immunotherapeutic clinical applications.read more
Citations
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Journal ArticleDOI
Minicircle DNA is Superior to Plasmid DNA in Eliciting Antigen-specific CD8+ T-cell Responses
Wynette M. Dietz,Nicole E. B. Skinner,Sara E. Hamilton,Michelle D. Jund,Suzanne M. Heitfeld,Adam J. Litterman,Patrick Hwu,Zhi-Ying Chen,Andres M. Salazar,John R. Ohlfest,Bruce R. Blazar,Christopher A. Pennell,Mark J. Osborn +12 more
TL;DR: It is shown for the first time that Minicircle DNA also functions as a vaccine platform, and intradermal delivery of MC DNA may prove more efficacious for prophylaxis than traditional pDNA vaccines.
Journal ArticleDOI
Intravital microscopy at the single vessel level brings new insights of vascular modification mechanisms induced by electropermeabilization.
Elisabeth Bellard,Bostjan Markelc,Sandrine Pelofy,Sandrine Pelofy,François Le Guerroué,François Le Guerroué,Gregor Sersa,Justin Teissié,Justin Teissié,Maja Cemazar,Muriel Golzio,Muriel Golzio +11 more
TL;DR: The aim of the present study was to understand and explain the effects of electropermeabilization on the dynamics (vasomotricity, permeability and recovery) of subcutaneous blood vessels towards different size of molecules.
Journal ArticleDOI
An engineered bispecific DNA-encoded IgG antibody protects against Pseudomonas aeruginosa in a pneumonia challenge model
Ami Patel,Antonio DiGiandomenico,Ashley E. Keller,Trevor R.F. Smith,Daniel H. Park,Stephanie Ramos,Katherine Schultheis,Sarah T. C. Elliott,Janess M. Mendoza,Kate E. Broderick,Megan C. Wise,Jian Yan,Jingjing Jiang,Seleeke Flingai,Amir S. Khan,Karuppiah Muthumani,Laurent Humeau,Lily Cheng,Leslie Wachter-Rosati,C. Kendall Stover,Niranjan Y. Sardesai,David B. Weiner +21 more
TL;DR: It is shown that two DMAbs targeting Pseudomonas aeruginosa proteins confer protection against lethal pneumonia in mice, supporting DNA-delivered monoclonal antibodies delivery as a potential strategy to augment the host immune response to prevent serious bacterial infections.
Journal ArticleDOI
DNA vaccines for SARS-CoV-2: toward third-generation vaccination era.
TL;DR: In this paper, the authors proposed a DNA vaccine platform for the SARS-CoV-2 pandemic outbreak 2019 (COVID-19) that is safe, cost effective, easy to produce, and most importantly induce appropriate immune responses and protection against viral infection.
Journal ArticleDOI
CCL19 and CCL28 Augment Mucosal and Systemic Immune Responses to HIV-1 gp140 by Mobilizing Responsive Immunocytes into Secondary Lymph Nodes and Mucosal Tissue
Kai Hu,Sukun Luo,Lina Tong,Xin Huang,Wei Jin,Wenjie Huang,Tao Du,Yan Yan,Siyi He,George E. Griffin,Robin J. Shattock,Qinxue Hu,Qinxue Hu +12 more
TL;DR: PCCL19 and pCCL28 can enhance HIV-1 envelope–specific systemic and mucosal Ab responses, as well as T cell responses, and such enhancements appear to be associated with mobilization of responsive immunocytes into secondary lymphoid organs and mucosa tissues through interactions with corresponding receptors.
References
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Vaccination with ALVAC and AIDSVAX to Prevent HIV-1 Infection in Thailand
Supachai Rerks-Ngarm,Punnee Pitisuttithum,Sorachai Nitayaphan,Jaranit Kaewkungwal,Joseph Chiu,Robert Paris,Nakorn Premsri,Chawetsan Namwat,Mark de Souza,Elizabeth Adams,Michael Benenson,Sanjay Gurunathan,Jim Tartaglia,John G. McNeil,Donald P. Francis,Donald Stablein,Deborah L. Birx,Supamit Chunsuttiwat,Chirasak Khamboonruang,Prasert Thongcharoen,Merlin L. Robb,Nelson L. Michael,Prayura Kunasol,Jerome H. Kim +23 more
TL;DR: This ALVAC-HIV and AIDSVAX B/E vaccine regimen may reduce the risk of HIV infection in a community-based population with largely heterosexual risk and offer insight for future research.
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Heterologous protection against influenza by injection of DNA encoding a viral protein
Jeffrey B. Ulmer,John J. Donnelly,Suezanne E. Parker,Gary Rhodes,Philip L. Felgner,V. J. Dwarki,Stanislaw H. Gromkowski,R. Randall Deck,Corrille M. DeWitt,Arthur Friedman,Linda A. Hawe,Karen R. Leander,Douglas Martinez,Helen C. Perry,John W. Shiver,Donna L. Montgomery,Margaret A. Liu +16 more
TL;DR: To generate a viral antigen for presentation to the immune system without the limitations of direct peptide delivery or viral vectors, plasmid DNA encoding influenza A nucleop protein was injected into the quadriceps of BALB/c mice and resulted in the generation of nucleoprotein-specific CTLs.
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Efficacy assessment of a cell-mediated immunity HIV-1 vaccine (the Step Study): a double-blind, randomised, placebo-controlled, test-of-concept trial.
Susan Buchbinder,Devan V. Mehrotra,Ann Duerr,Daniel W. Fitzgerald,Robin Mogg,David Li,Peter B. Gilbert,Javier R. Lama,Michael Marmor,Carlos del Rio,M. Juliana McElrath,Danilo R. Casimiro,Keith Gottesdiener,Chodakewitz Jeffrey A,Lawrence Corey,Michael N. Robertson +15 more
TL;DR: This cell-mediated immunity vaccine did not prevent HIV-1 infection or reduce early viral level and Mechanisms for insufficient efficacy of the vaccine and the increased HIV- 1 infection rates in subgroups of vaccine recipients are being explored.
Journal ArticleDOI
Genetic immunization is a simple method for eliciting an immune response.
TL;DR: It is reported that an immune response can be elicited by introducing the gene encoding a protein directly into the skin of mice by using a hand-held form of the biolistic system.
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DNA vaccines: protective immunizations by parenteral, mucosal, and gene-gun inoculations
Ellen F. Fynan,Robert G. Webster,Deborah H. Fuller,Joel R. Haynes,Joseph C. Santoro,Harriet L. Robinson +5 more
TL;DR: By far the most efficient DNA immunizations were achieved by using a gene gun to deliver DNA-coated gold beads to the epidermis, and 95% protection was achieved by two immunizations with beads loaded with as little as 0.4 micrograms of DNA.