Clinical Applications of DNA Vaccines: Current Progress
Bernadette Ferraro,Matthew P. Morrow,Natalie A. Hutnick,Thomas H. Shin,Colleen E. Lucke,David B. Weiner +5 more
TLDR
The ability of the current, or second-generation, DNA vaccines to induce more-potent cellular and humoral responses opens up this platform to be examined in both preventative and therapeutic arenas.Abstract:
It was discovered almost 20 years ago that plasmid DNA, when injected into the skin or muscle of mice, could induce immune responses to encoded antigens. Since that time, there has since been much progress in understanding the basic biology behind this deceptively simple vaccine platform and much technological advancement to enhance immune potency. Among these advancements are improved formulations and improved physical methods of delivery, which increase the uptake of vaccine plasmids by cells; optimization of vaccine vectors and encoded antigens; and the development of novel formulations and adjuvants to augment and direct the host immune response. The ability of the current, or second-generation, DNA vaccines to induce more-potent cellular and humoral responses opens up this platform to be examined in both preventative and therapeutic arenas. This review focuses on these advances and discusses both preventive and immunotherapeutic clinical applications.read more
Citations
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Immune response scenario and vaccine development for SARS-CoV-2 infection.
TL;DR: In this article, the authors described the natural immune response scenario during COVID-19 and the vaccines development trials to create efficient vaccines thus helping to build more effective approaches for prophylaxis and management.
Journal ArticleDOI
Control of SARS-CoV-2 infection after Spike DNA or Spike DNA+Protein co-immunization in rhesus macaques.
Margherita Rosati,Mahesh Agarwal,Xintao Hu,Santhi Devasundaram,Dimitris Stellas,Bhabadeb Chowdhury,Jenifer Bear,Robert Burns,Duncan Donohue,Laurent Pessaint,Hanne Leth Andersen,Mark G. Lewis,Evangelos Terpos,Meletios A. Dimopoulos,Alexander Wlodawer,James I. Mullins,David Venzon,George N. Pavlakis,Barbara K. Felber +18 more
TL;DR: In this article, the authors tested the immunogenicity and protective efficacy of DNA-based vaccine regimens expressing different prefusion-stabilized Wuhan-Hu-1 SARS-CoV-2 Spike antigens upon intramuscular injection followed by electroporation in rhesus macaques.
Journal ArticleDOI
[Rabies vaccines: Current status and prospects for development].
Elizaveta Starodubova,O. V. Preobrazhenskaia,Y. V. Kuzmenko,A. A. Latanova,E. I. Yarygina,Vadim L. Karpov +5 more
TL;DR: The main modern trends in the development of rabies vaccines have been discussed and new-generation vaccines are being developed based on recombinant rabies virus strains or on the production of an individual recombinant Rabies antigen-glycoprotein (G protein).
Journal ArticleDOI
Impact of plasmid size on the purification of model plasmid DNA vaccines by phenyl membrane adsorbers.
TL;DR: Membrane chromatography experiments show that the strength of interaction of pDNA isoforms with HIC membrane adsorbers depends on size, and differences in relative binding strength were explored using a stepwise elution strategy of decreasing buffer conductivities in order to increase the purity of supercoiled (SC) p DNA isoforms.
Journal Article
Antitumor Response to a Codon-Optimized HPV-16 E7/HSP70 Fusion Antigen DNA Vaccine
TL;DR: It is concluded that the fusion DNA vaccines considerably enhanced specific cellular responses against HPV tumor model, and optimized E7 showed a notable immunogenicity and inhibitory effect on the reduction of tumor size.
References
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Vaccination with ALVAC and AIDSVAX to Prevent HIV-1 Infection in Thailand
Supachai Rerks-Ngarm,Punnee Pitisuttithum,Sorachai Nitayaphan,Jaranit Kaewkungwal,Joseph Chiu,Robert Paris,Nakorn Premsri,Chawetsan Namwat,Mark de Souza,Elizabeth Adams,Michael Benenson,Sanjay Gurunathan,Jim Tartaglia,John G. McNeil,Donald P. Francis,Donald Stablein,Deborah L. Birx,Supamit Chunsuttiwat,Chirasak Khamboonruang,Prasert Thongcharoen,Merlin L. Robb,Nelson L. Michael,Prayura Kunasol,Jerome H. Kim +23 more
TL;DR: This ALVAC-HIV and AIDSVAX B/E vaccine regimen may reduce the risk of HIV infection in a community-based population with largely heterosexual risk and offer insight for future research.
Journal ArticleDOI
Heterologous protection against influenza by injection of DNA encoding a viral protein
Jeffrey B. Ulmer,John J. Donnelly,Suezanne E. Parker,Gary Rhodes,Philip L. Felgner,V. J. Dwarki,Stanislaw H. Gromkowski,R. Randall Deck,Corrille M. DeWitt,Arthur Friedman,Linda A. Hawe,Karen R. Leander,Douglas Martinez,Helen C. Perry,John W. Shiver,Donna L. Montgomery,Margaret A. Liu +16 more
TL;DR: To generate a viral antigen for presentation to the immune system without the limitations of direct peptide delivery or viral vectors, plasmid DNA encoding influenza A nucleop protein was injected into the quadriceps of BALB/c mice and resulted in the generation of nucleoprotein-specific CTLs.
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Efficacy assessment of a cell-mediated immunity HIV-1 vaccine (the Step Study): a double-blind, randomised, placebo-controlled, test-of-concept trial.
Susan Buchbinder,Devan V. Mehrotra,Ann Duerr,Daniel W. Fitzgerald,Robin Mogg,David Li,Peter B. Gilbert,Javier R. Lama,Michael Marmor,Carlos del Rio,M. Juliana McElrath,Danilo R. Casimiro,Keith Gottesdiener,Chodakewitz Jeffrey A,Lawrence Corey,Michael N. Robertson +15 more
TL;DR: This cell-mediated immunity vaccine did not prevent HIV-1 infection or reduce early viral level and Mechanisms for insufficient efficacy of the vaccine and the increased HIV- 1 infection rates in subgroups of vaccine recipients are being explored.
Journal ArticleDOI
Genetic immunization is a simple method for eliciting an immune response.
TL;DR: It is reported that an immune response can be elicited by introducing the gene encoding a protein directly into the skin of mice by using a hand-held form of the biolistic system.
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DNA vaccines: protective immunizations by parenteral, mucosal, and gene-gun inoculations
Ellen F. Fynan,Robert G. Webster,Deborah H. Fuller,Joel R. Haynes,Joseph C. Santoro,Harriet L. Robinson +5 more
TL;DR: By far the most efficient DNA immunizations were achieved by using a gene gun to deliver DNA-coated gold beads to the epidermis, and 95% protection was achieved by two immunizations with beads loaded with as little as 0.4 micrograms of DNA.