CRISPR-Barcoding for Intratumor Genetic Heterogeneity Modeling and Functional Analysis of Oncogenic Driver Mutations.
Alexis Guernet,Sathish Kumar Mungamuri,Dorthe Cartier,Ravi Sachidanandam,Anitha D. Jayaprakash,Sahil Adriouch,Myriam Vezain,Françoise Charbonnier,Guy Rohkin,Sophie Coutant,Shen Yao,Hassan Ainani,David Alexandre,Isabelle Tournier,Olivier Boyer,Stuart A. Aaronson,Youssef Anouar,Luca Grumolato +17 more
TLDR
This work devised a strategy to recapitulate and trace the emergence of subpopulations of cancer cells containing a mutation of interest using CRISPR/Cas9 technology and specific DNA barcodes, and used highly complex barcodes inserted at a specific genome location as a means of simultaneously tracing the fates of many thousands of genetically labeled cancer cells.About:
This article is published in Molecular Cell.The article was published on 2016-08-04 and is currently open access. It has received 58 citations till now. The article focuses on the topics: CRISPR & Cas9.read more
Citations
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A view on drug resistance in cancer
TL;DR: A reductionist approach is taken to define and separate the key determinants of drug resistance, which include tumour burden and growth kinetics; tumour heterogeneity; physical barriers; the immune system and the microenvironment; undruggable cancer drivers; and the many consequences of applying therapeutic pressures.
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Whole-organism clone tracing using single-cell sequencing
TL;DR: ScarTrace is presented, a single-cell sequencing strategy that enables the simultaneous quantification of clonal history and cell type for thousands of cells obtained from different organs of the adult zebrafish and predicts that similar approaches will have major applications in other experimental systems, in which the matching of embryonic clonal origin to adult cell type will ultimately allow reconstruction of how the adult body is built from a single cell.
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Tissue-specific tumorigenesis: context matters.
TL;DR: Investigation, recognition and in-depth biological understanding of the molecular, cellular, systemic and environmental determinants of organ-specific tumorigenesis and the mechanisms of context-specific oncogenic signalling outputs are focused on.
Journal ArticleDOI
Overview on Clinical Relevance of Intra-Tumor Heterogeneity.
Giorgio Stanta,Serena Bonin +1 more
TL;DR: This review tries to describe all the types of intra-tumor heterogeneity including morphohistological ITH, and at the molecular level clonal ITH derived from genomic instability and nonclonal IH derived from microenvironment interaction.
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CRISPR in cancer biology and therapy
TL;DR: A review of the progress made in the development of CRISPR systems as a tool to study cancer, and the emerging adaptation of these technologies to improve diagnosis and treatment can be found in this article .
References
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A new mathematical model for relative quantification in real-time RT-PCR.
TL;DR: This study enters into the particular topics of the relative quantification in real-time RT-PCR of a target gene transcript in comparison to a reference gene transcript and presents a new mathematical model that needs no calibration curve.
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Genome engineering using the CRISPR-Cas9 system
F. Ann Ran,Patrick D. Hsu,Jason Wright,Vineeta Agarwala,Vineeta Agarwala,David A. Scott,Feng Zhang +6 more
TL;DR: A set of tools for Cas9-mediated genome editing via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, as well as generation of modified cell lines for downstream functional studies are described.
Journal ArticleDOI
Intratumor heterogeneity and branched evolution revealed by multiregion sequencing.
Marco Gerlinger,Andrew Rowan,Stuart Horswell,James Larkin,David Endesfelder,Eva Grönroos,Pierre Martinez,Nicholas Matthews,Aengus Stewart,Patrick S. Tarpey,Ignacio Varela,Benjamin Phillimore,Sharmin Begum,Neil Q. McDonald,Adam Butler,David T. Jones,Keiran Raine,Calli Latimer,Claudio R. Santos,Mahrokh Nohadani,Aron Charles Eklund,Bradley Spencer-Dene,Graham Clark,Lisa Pickering,Gordon Stamp,Martin Gore,Zoltan Szallasi,Zoltan Szallasi,Julian Downward,P. Andrew Futreal,Charles Swanton +30 more
TL;DR: Intratumor heterogeneity can lead to underestimation of the tumor genomics landscape portrayed from single tumor-biopsy samples and may present major challenges to personalized-medicine and biomarker development.
Journal ArticleDOI
The clonal evolution of tumor cell populations
TL;DR: Each patient's cancer may require individual specific therapy, and even this may be thwarted by emergence of a genetically variant subline resistant to the treatment, which should be directed toward understanding and controlling the evolutionary process in tumors before it reaches the late stage usually seen in clinical cancer.
Journal ArticleDOI
The DNA-damage response in human biology and disease
Stephen P. Jackson,Jiri Bartek +1 more
TL;DR: The authors' improving understanding of DNA-damage responses is providing new avenues for disease management, and these responses are biologically significant because they prevent diverse human diseases.
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CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling
Randall Jeffrey Platt,Sidi Chen,Yang Zhou,Michael Yim,Lukasz Swiech,Hannah R. Kempton,James E. Dahlman,Oren Parnas,Thomas Eisenhaure,Marko Jovanovic,Daniel B. Graham,Siddharth Jhunjhunwala,Matthias Heidenreich,Ramnik J. Xavier,Robert Langer,Daniel G. Anderson,Nir Hacohen,Aviv Regev,Guoping Feng,Phillip A. Sharp,Feng Zhang +20 more