Dissecting human disease with single-cell omics: application in model systems and in the clinic.
Paulina M. Strzelecka,Paulina M. Strzelecka,Paulina M. Strzelecka,Anna Maria Ranzoni,Anna Maria Ranzoni,Anna Maria Ranzoni,Ana Cvejic +6 more
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TLDR
The current state of the art of single-cell techniques and their potential applications in deciphering the heterogeneous nature of diseases and tailoring personalised therapies are summarized.Abstract:
Probing cellular population diversity at single-cell resolution became possible only in recent years. The popularity of single-cell 'omic' approaches, which allow researchers to dissect sample heterogeneity and cell-to-cell variation, continues to grow. With continuous technological improvements, single-cell omics are becoming increasingly prevalent and contribute to the discovery of new and rare cell types, and to the deciphering of disease pathogenesis and outcome. Animal models of human diseases have significantly facilitated our understanding of the mechanisms driving pathologies and resulted in the development of more efficient therapies. The application of single-cell omics to animal models improves the precision of the obtained insights, and brings single-cell technology closer to the clinical field. This Review focuses on the use of single-cell omics in cellular and animal models of diseases, as well as in samples from human patients. It also highlights the potential of these approaches to further improve the diagnosis and treatment of various pathologies, and includes a discussion of the advantages and remaining challenges in implementing these technologies into clinical practice.read more
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Single-cell RNA sequencing in cardiovascular development, disease and medicine.
David T. Paik,David T. Paik,Sangkyun Cho,Sangkyun Cho,Lei Tian,Lei Tian,Howard Y. Chang,Joseph C. Wu,Joseph C. Wu +8 more
TL;DR: The currently available scRNA-seq technologies and analytical tools are summarized and the latest findings using sc RNA-seq that have substantially improved knowledge on the development of the cardiovascular system and the mechanisms underlying cardiovascular diseases are discussed.
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Cell engineering: Biophysical regulation of the nucleus.
TL;DR: This review will highlight the recent progress in nuclear biomechanics and mechanobiology in the context of cell engineering, tissue remodeling and disease development.
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Materials control of the epigenetics underlying cell plasticity
TL;DR: How materials guide the cellular epigenetic landscape is explored and how engineered materials target cell plasticity is discussed, particularly through dynamic changes in histone methylation and acetylation.
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Single Cell Metabolomics: A Future Tool to Unmask Cellular Heterogeneity and Virus-Host Interaction in Context of Emerging Viral Diseases.
TL;DR: Single cell metabolomics (SCM) emerges as necessity to explore such minute details of virus-host interface and the potential of SCM has already been exploited to resolve several biological conundrums.
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Advances in Chromatin and Chromosome Research: Perspectives from Multiple Fields
Andrews Akwasi Agbleke,Assaf Amitai,Jason D. Buenrostro,Aditi Chakrabarti,Lingluo Chu,Anders S. Hansen,Kristen M. Koenig,Ajay S. Labade,Sirui Liu,Tadasu Nozaki,Sergey Ovchinnikov,Andrew Seeber,Haitham A. Shaban,Jan-Hendrik Spille,Andrew D. Stephens,Jun-Han Su,Dushan N. Wadduwage +16 more
TL;DR: Technologies that will be crucial to interrogate key questions in chromatin and chromosome biology are shone a spotlight on including state-of-the-art microscopy techniques, tools to physically manipulate chromatin, single-cell methods to measure chromatin accessibility, computational imaging with neural networks and analytical tools to interpret chromatin structure and dynamics.
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