Dynamics and diversity in autophagy mechanisms: lessons from yeast
Reads0
Chats0
TLDR
The discovery of autophagy in yeast and the genetic tractability of this organism have allowed us to identify genes that are responsible for this process, which has led to the explosive growth of this research field seen today.Abstract:
Autophagy is a fundamental function of eukaryotic cells and is well conserved from yeast to humans. The most remarkable feature of autophagy is the synthesis of double membrane-bound compartments that sequester materials to be degraded in lytic compartments, a process that seems to be mechanistically distinct from conventional membrane traffic. The discovery of autophagy in yeast and the genetic tractability of this organism have allowed us to identify genes that are responsible for this process, which has led to the explosive growth of this research field seen today. Analyses of autophagy-related (Atg) proteins have unveiled dynamic and diverse aspects of mechanisms that underlie membrane formation during autophagy.read more
Citations
More filters
Journal ArticleDOI
Lysosomal Proteolysis Inhibition Selectively Disrupts Axonal Transport of Degradative Organelles and Causes an Alzheimer's-Like Axonal Dystrophy
TL;DR: The AD-associated defects in neuronal lysosomal proteolysis are identified as a possible basis for the selective transport abnormalities and highly characteristic pattern of neuritic dystrophy associated with AD.
Journal ArticleDOI
Mechanisms governing autophagosome biogenesis.
TL;DR: This work has shown that autophagosome biogenesis is a highly complex process, in which multiple proteins and lipids from various membrane sources, supported by the formation of membrane contact sites, cooperate with biophysical phenomena, including membrane shaping and liquid–liquid phase separation, to ensure seamless segregation of the autophagic cargo.
Journal ArticleDOI
The Ubiquitin–Proteasome System of Saccharomyces cerevisiae
TL;DR: The diverse effects of ubiquitylation reflect the assembly of structurally distinct ubiquitin chains on target proteins.
Journal ArticleDOI
De novo mutations in the autophagy gene WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood
Hirotomo Saitsu,Taki Nishimura,Taki Nishimura,Kazuhiro Muramatsu,Hirofumi Kodera,Satoko Kumada,Kenji Sugai,Emi Kasai-Yoshida,Noriko Sawaura,Hiroya Nishida,Ai Hoshino,Fukiko Ryujin,Seiichiro Yoshioka,Kiyomi Nishiyama,Yukiko Kondo,Yoshinori Tsurusaki,Mitsuko Nakashima,Noriko Miyake,Hirokazu Arakawa,Mitsuhiro Kato,Noboru Mizushima,Noboru Mizushima,Naomichi Matsumoto +22 more
TL;DR: Findings provide direct evidence that an autophagy defect is indeed associated with a neurodegenerative disorder in humans.
Journal ArticleDOI
Autophagy and Cancer Therapy
Paola Maycotte,Andrew Thorburn +1 more
TL;DR: Recent research related to autophagy and cancer therapy is discussed with a focus on how these processes may be manipulated to improve cancer therapy.
References
More filters
Journal ArticleDOI
LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing
Yukiko Kabeya,Noboru Mizushima,Noboru Mizushima,Takashi Ueno,Akitsugu Yamamoto,Takayoshi Kirisako,Takayoshi Kirisako,Takeshi Noda,Takeshi Noda,Eiki Kominami,Yoshinori Ohsumi,Yoshinori Ohsumi,Tamotsu Yoshimori,Tamotsu Yoshimori +13 more
TL;DR: It is demonstrated that the rat microtubule‐associated protein 1 light chain 3 (LC3), a homologue of Apg8p essential for autophagy in yeast, is associated to the autophagosome membranes after processing.
Journal ArticleDOI
Autophagy fights disease through cellular self-digestion
TL;DR: Understanding autophagy may ultimately allow scientists and clinicians to harness this process for the purpose of improving human health, and to play a role in cell death.
Journal ArticleDOI
TOR signaling in growth and metabolism.
TL;DR: The physiological consequences of mammalianTORC1 dysregulation suggest that inhibitors of mammalian TOR may be useful in the treatment of cancer, cardiovascular disease, autoimmunity, and metabolic disorders.
Journal ArticleDOI
p62/SQSTM1 Binds Directly to Atg8/LC3 to Facilitate Degradation of Ubiquitinated Protein Aggregates by Autophagy
Serhiy Pankiv,Terje Høyvarde Clausen,Trond Lamark,Andreas Brech,Jack-Ansgar Bruun,Heidi Outzen,Aud Øvervatn,Geir Bjørkøy,Terje Johansen +8 more
TL;DR: It is demonstrated that the previously reported aggresome-like induced structures containing ubiquitinated proteins in cytosolic bodies are dependent on p62 for their formation and p62 is required both for the formation and the degradation of polyubiquitin-containing bodies by autophagy.
Journal ArticleDOI
Autophagy: process and function
TL;DR: In this review, the process of autophagy is summarized, and the role of autophileagy is discussed in a process-based manner.