Dynamics and diversity in autophagy mechanisms: lessons from yeast
Reads0
Chats0
TLDR
The discovery of autophagy in yeast and the genetic tractability of this organism have allowed us to identify genes that are responsible for this process, which has led to the explosive growth of this research field seen today.Abstract:
Autophagy is a fundamental function of eukaryotic cells and is well conserved from yeast to humans. The most remarkable feature of autophagy is the synthesis of double membrane-bound compartments that sequester materials to be degraded in lytic compartments, a process that seems to be mechanistically distinct from conventional membrane traffic. The discovery of autophagy in yeast and the genetic tractability of this organism have allowed us to identify genes that are responsible for this process, which has led to the explosive growth of this research field seen today. Analyses of autophagy-related (Atg) proteins have unveiled dynamic and diverse aspects of mechanisms that underlie membrane formation during autophagy.read more
Citations
More filters
Journal ArticleDOI
Eradication of intracellular Francisella tularensis in THP-1 human macrophages with a novel autophagy inducing agent
Hao-Chieh Chiu,Shilpa Soni,Samuel K. Kulp,Heather M. Curry,Dasheng Wang,John S. Gunn,Larry S. Schlesinger,Ching-Shih Chen +7 more
TL;DR: Together, AR-12 represents a proof-of-principle that intracellular F. tularensis can be eradicated by small-molecule agents that target innate immunity.
Journal ArticleDOI
The Crucial Role of Atg5 in Cortical Neurogenesis During Early Brain Development
TL;DR: The results revealed that Atg5 has a crucial role in cortical neurogenesis during early embryonic brain development, which may contribute to the understanding of neurodevelopmental disorders caused by autophagy dysregulation.
Journal ArticleDOI
Molecular basis of ubiquitin recognition by the autophagy receptor CALCOCO2
Xingqiao Xie,Faxiang Li,Yuanyuan Wang,Yingli Wang,Zhijie Lin,Xiaofang Cheng,Jianping Liu,Changbin Chen,Lifeng Pan +8 more
TL;DR: The structure of the CALCOCO2 C2H2-type zinc finger in complex with mono-ubiquitin also uncovers a unique zinc finger-binding mode for ubiquitin, providing mechanistic insight into how CALC OCO2 targets ubiquitIn-decorated pathogens for autophagic degradations.
Journal ArticleDOI
The p53-induced Gene Ei24 Is an Essential Component of the Basal Autophagy Pathway
TL;DR: It is demonstrated that Ei24 is an essential autophagy gene and plays an important role in clearance of aggregate-prone proteins in neurons and hepatocytes and in accumulation of LC3, p62 aggregates, and ubiquitin-positive inclusions.
Journal ArticleDOI
A Selective Autophagy Pathway for Phase-Separated Endocytic Protein Deposits.
Florian Wilfling,Chia-Wei Lee,Philipp Erdmann,Yumei Zheng,Dawafuti Sherpa,Stefan Jentsch,Boris Pfander,Brenda A. Schulman,Wolfgang Baumeister +8 more
TL;DR: A selective autophagy pathway for protein condensates formed by endocytic proteins in yeast is reported and a model for autophagic degradation of macromolecular protein complexes by the action of intrinsic Autophagy receptors is suggested.
References
More filters
Journal ArticleDOI
LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing
Yukiko Kabeya,Noboru Mizushima,Noboru Mizushima,Takashi Ueno,Akitsugu Yamamoto,Takayoshi Kirisako,Takayoshi Kirisako,Takeshi Noda,Takeshi Noda,Eiki Kominami,Yoshinori Ohsumi,Yoshinori Ohsumi,Tamotsu Yoshimori,Tamotsu Yoshimori +13 more
TL;DR: It is demonstrated that the rat microtubule‐associated protein 1 light chain 3 (LC3), a homologue of Apg8p essential for autophagy in yeast, is associated to the autophagosome membranes after processing.
Journal ArticleDOI
Autophagy fights disease through cellular self-digestion
TL;DR: Understanding autophagy may ultimately allow scientists and clinicians to harness this process for the purpose of improving human health, and to play a role in cell death.
Journal ArticleDOI
TOR signaling in growth and metabolism.
TL;DR: The physiological consequences of mammalianTORC1 dysregulation suggest that inhibitors of mammalian TOR may be useful in the treatment of cancer, cardiovascular disease, autoimmunity, and metabolic disorders.
Journal ArticleDOI
p62/SQSTM1 Binds Directly to Atg8/LC3 to Facilitate Degradation of Ubiquitinated Protein Aggregates by Autophagy
Serhiy Pankiv,Terje Høyvarde Clausen,Trond Lamark,Andreas Brech,Jack-Ansgar Bruun,Heidi Outzen,Aud Øvervatn,Geir Bjørkøy,Terje Johansen +8 more
TL;DR: It is demonstrated that the previously reported aggresome-like induced structures containing ubiquitinated proteins in cytosolic bodies are dependent on p62 for their formation and p62 is required both for the formation and the degradation of polyubiquitin-containing bodies by autophagy.
Journal ArticleDOI
Autophagy: process and function
TL;DR: In this review, the process of autophagy is summarized, and the role of autophileagy is discussed in a process-based manner.